Characterisation and expression of myogenesis regulatory factors during in vitro myoblast development and in vivo fasting in the gilthead sea bream (Sparus aurata)

serrana, Daniel García de la; Codina, Marta; Capilla, Encarnación; Jiménez-Amilburu, Vanesa; Navarro, Isabel; Du, Shao Jun; Johnston, Ian A.; Gutiérrez, Joaquím

doi:10.1016/j.cbpa.2013.10.020

Cited by 56 publications

(51 citation statements)

References 54 publications

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“…The expression in bone and muscle of the inhibitor myostatin (mstn2) showed an inverse profile suggesting an inter-organ coordination that needs to be further explored in fish. Overall, this study provides new information on the molecules involved in the musculoskeletal system remodeling during the early stages of refeeding in fish.conditions has been characterized and the ratio of the circulating levels of Gh and Igf1 is considered a good marker of growth quality in terms of its relation with body indexes or muscle fibers composition 12,13 .Muscle growth is also controlled by myogenic regulatory factors (MRFs) (MyoD, Myf5, Myog and Mrf4), and the negative endogenous regulator myostatin, as well as other factors that control sequentially the process of development and growth 6,14,15 . The effects of fasting and refeeding on myogenesis have been studied in diverse fish species 6,10,16 demonstrating the function of the different MRFs during the metabolic arrest caused with food limitation and the subsequent activation when feeding is restored.…”

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confidence: 99%

Regulatory mechanisms involved in muscle and bone remodeling during refeeding in gilthead sea bream

Lavajoo

Perelló-Amorós

Vélez

et al. 2020

Sci Rep

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The tolerance of fish to fasting offers a model to study the regulatory mechanisms and changes produced when feeding is restored. Gilthead sea bream juveniles were exposed to a 21-days fasting period followed by 2 h to 7-days refeeding. Fasting provoked a decrease in body weight, somatic indexes, and muscle gene expression of members of the Gh/Igf system, signaling molecules (akt, tor and downstream effectors), proliferation marker pcna, myogenic regulatory factors, myostatin, and proteolytic molecules such as cathepsins or calpains, while most ubiquitin-proteasome system members increased or remained stable. In bone, downregulated expression of Gh/Igf members and osteogenic factors was observed, whereas expression of the osteoclastic marker ctsk was increased. Refeeding recovered the expression of Gh/Igf system, myogenic and osteogenic factors in a sequence similar to that of development. Akt and Tor phosphorylation raised at 2 and 5 h post-refeeding, much faster than its gene expression increased, which occurred at day 7. The expression in bone and muscle of the inhibitor myostatin (mstn2) showed an inverse profile suggesting an inter-organ coordination that needs to be further explored in fish. Overall, this study provides new information on the molecules involved in the musculoskeletal system remodeling during the early stages of refeeding in fish.conditions has been characterized and the ratio of the circulating levels of Gh and Igf1 is considered a good marker of growth quality in terms of its relation with body indexes or muscle fibers composition 12,13 .Muscle growth is also controlled by myogenic regulatory factors (MRFs) (MyoD, Myf5, Myog and Mrf4), and the negative endogenous regulator myostatin, as well as other factors that control sequentially the process of development and growth 6,14,15 . The effects of fasting and refeeding on myogenesis have been studied in diverse fish species 6,10,16 demonstrating the function of the different MRFs during the metabolic arrest caused with food limitation and the subsequent activation when feeding is restored. Furthermore, during fasting and refeeding, proteolytic molecules play a remarkable role to adapt to the changes in amino acids supply. This is more important in fish that have a specific and enhanced protein metabolism. The main endogenous proteolytic systems, each one performing specific degradative or regulatory functions according to the nutritional conditions are, calpains, cathepsins and ubiquitin-proteasome, all of which are well-known in fish, especially salmonids [17][18][19] , but also in gilthead sea bream [20][21][22] .In addition to muscle, bone is also an important tissue for skeletal and locomotion functions, but also, as a reservoir of minerals that is clearly affected when nutrition is restricted. Essential during development, synchronicity between bone and muscle is required for proper musculoskeletal growth 23,24 . Besides to being induced by the Gh/Igf system, the process of osteogenesis is also regulated by skeleton-derived factors that...

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mentioning

confidence: 99%

Regulatory mechanisms involved in muscle and bone remodeling during refeeding in gilthead sea bream

Lavajoo

Perelló-Amorós

Vélez

et al. 2020

Sci Rep

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“…In agreement with this observation, PCR experiments showed that levels of myogenin promoter-driven GFP transcript and myogenin transcripts were similar in muscle from fasted and fed trout. Similarly, it has been shown that myogenin expression is not affected after fasting in juvenile gilthead sea bream (Garcia de la Serrana et al, 2014). In line with the lasting production of myofibres in atrophied muscle, it is interesting to note that the catabolic response induced by shortterm calorie restriction is favourable to myogenic cell activation and muscle repair in mouse (Cerletti et al, 2012).…”

Section: Discussionmentioning

confidence: 77%

Analysis of muscle fibre input dynamics using amyog: GFP transgenic trout model

Rescan

Rallière

Lebret

et al. 2015

Journal of Experimental Biology

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The dramatic increase in myotomal muscle mass in teleosts appears to be related to their sustained ability to produce new fibres in the growing myotomal muscle. To describe muscle fibre input dynamics in trout (Oncorhynchus mykiss), we generated a stable transgenic line carrying green fluorescent protein (GFP) cDNA driven by the myogenin promoter. In this myog:GFP transgenic line, muscle cell recruitment is revealed by the appearance of fluorescent, small, nascent muscle fibres. The myog:GFP transgenic line displayed fibre formation patterns in the developing trout and showed that the production of new fluorescent myofibres (muscle hyperplasia) is prevalent in the juvenile stage but progressively decreases to eventually cease at approximately 18 months post-fertilisation. However, fluorescent, nascent myofibres were formed de novo in injured muscle of aged trout, indicating that the inhibition of myofibre formation associated with trout ageing cannot be attributed to the lack of recruitable myogenic cells but rather to changes in the myogenic cell microenvironment. Additionally, the myog:GFP transgenic line demonstrated that myofibre production persists during starvation.

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“…Furthermore, myogenin levels in Nile tilapia juveniles measured at the first 2 weeks of fasting (2WF) and after 10 weeks of refeeding (10WR) did not differ in relation to FC. In gilthead sea bream, myogenin gene expression was also found to not be affected after 30 days of food deprivation (Garcia et al 2014). MyoD expression can be expected to be reduced in fasting fish as the lack of available nutrient energy would preclude muscle development and growth.…”

Section: Discussionmentioning

confidence: 97%

Alteration in expression of atrogenes and IGF-1 induced by fasting in Nile tilapia Oreochromis niloticus juveniles

et al. 2017

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The growth rate of farmed fish is an important factor regarding aquaculture success. An understanding of the cellular events that occur in skeletal muscle when fish undergo periods of fasting and refeeding provides information useful in developing alternative feeding strategies for improving muscle growth in commercially cultivated species. To evaluate the effect of 1-3 weeks of fasting and 10 weeks of refeeding in Nile tilapia juveniles, we analyzed the growth performance and changes in muscle cellularity and the expression of the following growth and muscle related genes: MyoD, myogenin, IGF-1, IGF-1 receptor, MuRF-1, atrogin-1 and myostatin. Reduced body mass was observed in all three groups of fasted fish during their time off feed, and 10 weeks of refeeding resulted in partial compensatory growth of body mass. No differences in the frequency of white muscle fiber diameters were observed between fasted and fed control fish treatments. However, changes in gene expression induced by fasting and refeeding were found. IGF-1 receptor, ubiquitin ligases MuRF1 and atrogin-1 expression increased during the 1-3 weeks of fasting, while IGF-1 levels dropped significantly (P \ 0.001) compared to the control treatment. Furthermore, myogenin mRNA level in fish submitted to 3 weeks of fasting was higher in comparison to the control treatment (P \ 0.05). Overall, our results showed that 1-3 weeks of fasting can induce muscle atrophy activation in Nile tilapia juveniles, and 10 weeks of refeeding is enough to induce only partial compensatory growth.

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Characterisation and expression of myogenesis regulatory factors during in vitro myoblast development and in vivo fasting in the gilthead sea bream (Sparus aurata)

Cited by 56 publications

References 54 publications

Regulatory mechanisms involved in muscle and bone remodeling during refeeding in gilthead sea bream

Regulatory mechanisms involved in muscle and bone remodeling during refeeding in gilthead sea bream

Analysis of muscle fibre input dynamics using amyog: GFP transgenic trout model

Alteration in expression of atrogenes and IGF-1 induced by fasting in Nile tilapia Oreochromis niloticus juveniles

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