Characterisation and expression analysis of UBC9 and UBS27 genes in developing gonads of cicindelids (Coleoptera: Cicindelidae)

Rodríguez-García, María Juliana; García-Reina, Andrés; Machado, Vilmar; Galián, José

doi:10.1016/j.cbpb.2016.08.003

Cited by 1 publication

(4 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…littoralis after an infection, which seemed to increase its expression as it was showed in a previous work 37 , being in accordance to other studies performed for defensin genes in insects 51 , 52 . On the other hand, the ubiquitin UBC9 was tested for the rest of the conditions in both species, showing significant differences in the body parts comparison, primarily in gonads, as was previously checked in a different work 38 and in other insects such as Drosophila 53 , which confirmed the relation of this gene with cicindelids reproductive system.…”

Section: Discussionsupporting

confidence: 58%

“…campestris: (i) male and female beetle comparisons; (ii) four different body parts including head, thorax, abdomen and gonads; (iii) developmental stage of their gonads, discerned based on the description reported by Paarmann 54 and previously used by Rodríguez-García et al . 38 . The first two conditions were also tested for C .…”

Section: Methodsmentioning

confidence: 99%

“…littoralis (sex: Myo and Myo-Alc; infection: NADH and B-t; body parts: NADH, 60Sa and ArgKin). Both target genes were amplified as described in previous works with previously described primers 37 , 38 . UBC9, an ubiquitin gene related to reproduction and sexual activity, was tested for all conditions and species with the exception of the infection in C .…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, the genomic study of non-model species, especially with the modern technologies currently available, provides important information to understand the genetic basis of adaptive traits 35 . Nevertheless, only a few studies have been performed in cicindelids regarding the study of their transcriptome, in relation to their reproduction and innate immune response 36 – 38 but there is no information available concerning the stability of HKGs in this group of beetles. The availability of a selection of validated reference genes for genomic and expression analyses will allow us to obtain more reliable results in future studies.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Validation of reference genes for quantitative real-time PCR in tiger beetles across sexes, body parts, sexual maturity and immune challenge

García-Reina

Rodríguez-García

Galián

2018

Sci Rep

Self Cite

View full text Add to dashboard Cite

Reference genes are frequently used as normalizers for expression studies despite not being previously verified to present suitable stabilities. Considering the interest that tiger beetles have generated in the past years, resulting in a variety of studies, it is crucial to dispose of a validated reference gene panel for expression studies. Nine candidate genes were tested in Cicindela campestris and Calomera littoralis across several conditions and their transcription levels were assessed with geNorm, NormFinder, BestKeeper and ΔCT method algorithms. Results showed high stabilities across sexes, immune challenge and gonad developmental stages for all genes tested, while body parts comparison presented less constant expression values. Only two genes are sufficient to perform a proper normalization for most of the conditions tested, except for the body parts comparison in C. littoralis, which requires the use of at least three reference genes. On the whole, no universal gene is found to be suitable for all situations, but according to the acceptable range of values, NADH, B-t, Vatpase and ArgKin seem to present the most constant expression stability, indicating their suitability as reference genes in most of the conditions. This is the first report evaluating the stability of housekeeping genes in adephagan beetles.

show abstract