Chara braunii genome: a new resource for plant electrophysiology

Beilby, Mary J.

doi:10.1007/s12551-019-00512-7

Cited by 18 publications

(11 citation statements)

References 53 publications

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“…Impaling leads into the plant possibly obtains a better signal/noise ratio compared to surface leads due to the direct contact with the fluids within the stem of the plants as opposed to the contact through and between the cells on the surface of the stem to the internal fluids. Plants with large cells, such as green alga Chara , have made it easier in the past to know exactly where one is obtaining a recording (i.e., intracellular or extracellular compartments) [ 21 ]. Surface electrodes at least provide some overview in the field potential as well as a lead placed within the plant but not in a specific location.…”

Section: Discussionmentioning

confidence: 99%

Impedance Measures for Detecting Electrical Responses during Acute Injury and Exposure of Compounds to Roots of Plants

Cooper

Thomas

McLetchie

2022

MPs

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Electrical activity is widely used for assessing a plant’s response to an injury or environmental stimulus. Commonly, a differential electrode recording between silver wire leads with the reference wire connected to the soil, or a part of the plant, is used. One method uses KCl-filled glass electrodes placed into the plant, similar to recording membrane/cell potentials in animal tissues. This method is more susceptible to artifacts of equipment noise and photoelectric effects than an impedance measure. An impedance measure using stainless steel wires is not as susceptible to electrically induced noises. Impedance measurements are able to detect injury in plants as well as exposure of the roots to environmental compounds (glutamate). The impedance measures were performed in 5 different plants (tomato, eggplant, pepper, liverwort, and Coleus scutellarioides), and responses to mechanical movement of the plant, as well as injury, were recorded. Monitoring electrical activity in a plant that arises in a distant plant was also demonstrated using the impedance method. The purpose of this report is to illustrate the ease in using impedance measures for monitoring electrical signals from individual plants or aggregates of plants for potentially scaling for high throughput and monitoring controlled culturing and outdoor field environments.

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Section: Discussionmentioning

confidence: 99%

Impedance Measures for Detecting Electrical Responses during Acute Injury and Exposure of Compounds to Roots of Plants

Cooper

Thomas

McLetchie

2022

MPs

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“…Recently, the Chara braunii genome was published (28), expanding possible scientific approaches (29,30). Thus, Characeaen algae are not only complex organisms constantly providing new insights into plant electrophysiology, but also simple enough systems to be routinely used for student laboratory exercises.…”

Section: Discussionmentioning

confidence: 99%

Using Plant Cells of Nitellopsis obtusa for Biophysical Education

et al. 2021

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Using giant characeaen algae Nitellopsis obtusa in laboratory exercises is proposed to familiarize students with basic concepts of electrophysiology and provide some simple hands-on practice. The described concept experiments present extracellular registration of action potentials (APs) and investigation of cytoplasmic streaming properties. Students are expected to register the propagation velocity of APs (found to be 3.4 ± 1.5 cm/s in N. obtusa), as well as the velocity of cytoplasmic streaming (66.7 ± 9 μm/s). Proposed exercises also concern recovery dynamics of cytoplasmic streaming after a stimulation (recovery time constant τ = 3.7 ± 2.1 min) as well as investigation of an effect of various chemicals (e.g., KCl) on all selected parameters. The experiments endorse characeaen algae as a model system to be routinely explored in education of biophysics and bioelectrical phenomena of the cell.

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“…At the cell surface, areas with high charasome abundance stabilize and restrict the position of the acidic bands, whereas regions with low charasome abundance allow the formation of either stable or unstable alkaline bands ( Pertl-Obermeyer et al, 2018 ; Absolonova et al, 2019 ). This acidification phenomenon has made Chara and other Charophycean taxa outstanding organisms for electrophysiology studies at the cellular level ( Beilby and Casanova, 2014 ; Bulychev and Komarova, 2014 ; Beilby, 2016 , 2019 ).…”

Section: The Cell Biology Of Charophytesmentioning

confidence: 99%

The cell biology of charophytes: Exploring the past and models for the future

Domozych

Bagdan

2022

Plant Physiology

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Charophytes represent a diverse assemblage of extant green algae that are the sister lineage to land plants. 500-600+ million years ago, a charophyte progenitor successfully colonized land and subsequently gave rise to land plants. Charophytes have diverse but relatively simple body plans that make them highly attractive organisms for many areas of biological research. At the cellular level, many charophytes have been used for deciphering cytoskeletal networks and their dynamics, membrane trafficking, extracellular matrix secretion and cell division mechanisms. Some charophytes live in challenging habitats and have become excellent models for elucidating the cellular and molecular effects of various abiotic stressors on plant cells. Recent sequencing of several charophyte genomes has also opened doors for the dissection of biosynthetic and signaling pathways. While we are only in an infancy stage in elucidating the cell biology of charophytes, the future application of novel analytical methodologies in charophyte studies that include a broader survey of inclusive taxa will enhance our understanding of plant evolution and cell dynamics.

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Chara braunii genome: a new resource for plant electrophysiology

Cited by 18 publications

References 53 publications

Impedance Measures for Detecting Electrical Responses during Acute Injury and Exposure of Compounds to Roots of Plants

Impedance Measures for Detecting Electrical Responses during Acute Injury and Exposure of Compounds to Roots of Plants

Using Plant Cells of Nitellopsis obtusa for Biophysical Education

The cell biology of charophytes: Exploring the past and models for the future

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