Chapter VIII Serological Typing Methods of Leptospires

Zoonotic Infections Among Employees from Great Smoky Mountains and Rocky Mountain National Parks, 2008–2009

“…Pathogens tested and specific methods of serologic testing for each of the agents evaluated have been described elsewhere (Table 1; Lindsey et al 1976;Dikken and Kmety 1978;Brown et al 1981;Dalton et al 1995;Plikaytis et al 1996;Nicholson et al 1997;Johnson et al 2000Johnson et al , 2005Martin et al 2000;Quinn et al 2002Quinn et al , 2004Dumler 2004;Semenova et al 2004;Jones et al 2007). Baseline seropositivity was interpreted as prior infection.…”

Leptospira interrogans serovar Valbuzzi: a cause of severe pulmonary haemorrhages in the Andaman Islands

“…MAT was performed following standard procedures (Wolff, 1954) using a panel of 36 group-specific rabbit antisera (group sera) representing all pathogenic serogroups. An isolate was considered to belong to the serogroup of the group serum that gave the highest titre (Dikken & Kmety, 1978).…”

“…Each isolate was tested by MAT against all reference antisera of the identified serogroup to establish cross-reactivity patterns. CAAT was performed following a standard procedure (Dikken & Kmety, 1978) to identify the serovar status of the isolates. The Netherlands) to generate characteristic agglutination profiles, as described previously (Desmecht et al, 1991;Korver et al, 1988).…”

New Leptospira serovar Sokoine of serogroup Icterohaemorrhagiae from cattle in Tanzania

“…Cross-agglutination absorption test (CAAT), the gold standard test for serological classification of Leptospira serovars, was repeatedly carried out by the Sokoine University of Agriculture (Tanzania) as described elsewhere (Stallman, 1987;Dikken & Kmety, 1978 Results from this study revealed that isolate RM1 is a pathogenic Leptospira as indicated by suppressed growth both at 13 uC and in the presence of 8-azaguanine. PCR with pathogenic primers Lepat 1 and Lepat 2 gave a DNA product of 330 bp ( Supplementary Fig.…”