Chapter V Production and Isolation of Flagella

Smith, R. W.; Koffler, Henry

doi:10.1016/s0580-9517(08)70523-3

Cited by 11 publications

(4 citation statements)

References 53 publications

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“…At mid-exponential phase, they were harvested by centrifugation at 6,000 x g for 15 min at 40C and suspended in 20 ml of distilled water. Flagellar filaments were prepared by the method of Kamio and Terawaki (17) and disaggregated by 0.01 N HCl, and the flagellins were obtained by the method of Smith and Koffler (27).…”

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confidence: 99%

Outer membrane proteins and cell surface structure of Selenomonas ruminantium

Kamio¹,

Takahashi²

1980

J Bacteriol

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The protein compositions of the membrane preparations from Selenomonas ruminantium grown in glucose or lactate medium were determined by sodium dodecyl sulfateand two-dimensional (first, isoelectric focusing; second, sodium dodecyl sulfate) polyacrylamide slab gel electrophoresis. The outer membrane from both glucoseand lactate-grown cells contained two major proteins with apparent molecular weights of 42,000 and 40,000. These proteins existed as peptidoglycan-associated proteins in the outer membrane. The critical temperature at which they were dissociated completely into the monomeric subunits of 42,000 and 40,000 daltons was found to be 85°C. The amount of each protein varied considerably depending upon the cultural conditions. The absence of the lipoprotein of Braun in S. ruminantium was suggested in our preceding paper (Y. Kamio, and H. Takahashi, J. Bacteriol. 141:888-898, 1980), and the possible absence of the protein components corresponding to the Braun lipoprotein in this strain was confirmed by electrophoretic analysis of the outer membrane and the lysozyme-treated peptidoglycan fractions. Examination of the cell surface of S. ruminantium by electron microscopy showed that the outer membrane formed a wrinkled surface with irregular blebs, some of which pinched off forning vesicles of various sizes. Rapid cell lysis occurred with the addition of a low level of lysozyme to the cell suspension. These findings led us to conclude that the physiological and morphological properties of this strain were similar to those of "deep rough" and mlp or Ipo mutants of Escherichia coli K-12, respectively.

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Outer membrane proteins and cell surface structure of Selenomonas ruminantium

Kamio¹,

Takahashi²

1980

J Bacteriol

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“…The content of glycine, alanine, leucine, isoleucine, threonine, aspartic acid and glutamic acid is high in B.firmus RAB flagellin and that of other bacilli (Smith & Koffler, 1971b). In addition, little or no cysteine, tyrosine, tryptophan, proline and histidine residues are found in B.firmus RAB and related species.…”

Section: Results a N D Discussionmentioning

confidence: 93%

Purification and Characterization of Flagella from the Alkalophile Bacillus firmus RAB

Guffanti

Eisenstein

1983

Microbiology

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Flagella from Bacillus firmus RAB, an alkalophilic bacterium, were purified to homogeneity. The flagella were shown to consist of a single protein subunit (flagellin) with an apparent molecular weight of 40000. The amino acid composition of B.Jirmus RAB flagellin was similar to that of other bacilli except that the former had far fewer basic amino acids. The paucity of basic amino acids may render the flagella more stable at external pH values as high as 11.0.

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“…L. grayi and L. murrayi, which only have the E type flagellar antigen did not react with any of the monoclonal antibodies. In addition, L. denitrificans which will probably be placed out of the genus entirely was non-reactive (22,24). These reactivities are beneficial since none of the latter three bacteria are pathogenic for man.…”

Section: Discussionmentioning

confidence: 99%

Monoclonal Antibodies Directed Against the Flagellar Antigens of Listeria Species and Their Potential in EIA-Based Methods

Farber

Speirs

1987

Journal of Food Protection

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Monoclonal antibodies directed against antigens of Listeria spp. were produced. Three main classes of immunoglobulins were found that reacted with Listeria strains containing either the A, B, or C flagellar antigen. These antibodies reacted with Listeria monocytogenes, Listeria welshimeri, Listeria seeligeri, Listeria ivanovii and Listeria innocua, but not Listeria grayi, Listeria murrayi or Listeria denitrificans. The monoclones tested did not cross-react with any of the 30 non-Listeria cultures examined, including Staphylococcus aureus and Streptococcus faecalis. Cheese and milk samples naturally-contaminated with L. monocytogenes were found to be positive for Listeria within two working days after initiation by using the monoclonal antibodies in an enzyme immunoassay.

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Chapter V Production and Isolation of Flagella

Cited by 11 publications

References 53 publications

Outer membrane proteins and cell surface structure of Selenomonas ruminantium

Outer membrane proteins and cell surface structure of Selenomonas ruminantium

Purification and Characterization of Flagella from the Alkalophile Bacillus firmus RAB

Monoclonal Antibodies Directed Against the Flagellar Antigens of Listeria Species and Their Potential in EIA-Based Methods

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