Chapter II Bacteriocin Typing of Enterohacteriaceae

Gillies, Robert R.

doi:10.1016/s0580-9517(08)70487-2

Cited by 2 publications

(3 citation statements)

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“…Results with this series of isolates are in direct contrast to those of other workers who, with a 24-h primary incubation period for colicine production, observed neither quantitative nor qualitative differences in strains stored for many years (Gillies, 1964(Gillies, , 1978 OLD et al 1971). Because we followed the techniques of Gillies (1964Gillies ( , 1978 we are unable to offer any satisfactory explanation for these observed differences in type stability. Nevertheless, like Abbot & Shannon (1958), our results demonstrated the need for a period of primary incubation longer than 24 h for accurate colicine typing of isolates of Sh.…”

Section: Discussioncontrasting

confidence: 94%

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Comparison of four methods of differential typing of isolates ofShigella sonnei

Helgason

Old

1981

J. Hyg.

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SUMMARYAn epidemiological study of Sonne dysentery in Dundee during the years 1971-6 was made by examining, in respect of 1420 isolates of Shigella sonnei, the discriminating power of colicine typing, antibiogram testing, biotyping and resistotyping and the stability of the markers they provided.Colicine typing identified nine colicine types, including four not previously described. However, because types 4 and 4 var., determined by col Ib, and type U, producing no colicines, accounted for 960 of the isolates, discrimination with colicine typing was poor. In antibiotic sensitivity tests, 13 different antibiogram patterns were noted. Less than 1 % of the isolates were sensitive to all of the eight antibiotics tested; most were multiply drug-resistant. Resistance to kanamycin, neomycin and paromomycin (KNP) was apparently due to a single resistance determinant, widely distributed in a majority (5300) of the isolates. When definitive times were chosen for reading each biotyping test, only maltose and rhamnose of the 13 'sugars' tested differentiated isolates into prompt-and late-fermenting types. Though the ability to ferment rhamnose was a stable property, it discriminated only 1X50% of the minority, late-fermenting type.Resistotyping with six chemicals discriminated eight epidemiologically valid resistotypes, including three new types. However, 93 % of the isolates belonged to only three resistotypes.Analysis of the data for isolates from 286 epidemiologically distinct episodes showed that the variability of colicine and antibiogram characters, found among isolates within, respectively, 40 and 280 of the episodes, was generally associated with loss or gain of a plasmid ('col I b-KNP') which determined production of colicine I b and KNP resistance. These characters varied both in vivo and in vitro. Variability of resistotype characters, on the other hand, was observed in only 28 (9 %) episodes, 14 of which possibly represented examples of mixed or sequential infections.For accurate epidemiological tracing of strains of Sh. sonnei in a community, resistotyping, the technique showing the greatest discrimination and least variability of the four tested, should be included as the principal typing method.HYo 87 12

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Section: Discussioncontrasting

confidence: 94%

“…The technique of Abbott & Shannon (1958) was used with modifications as described by Gillies (1964Gillies ( , 1978. Isolates were tested for colicine production after primary incubation for 24 h at 35-5°C on tryptone soya agar (Oxoid CM131) supplemented with 5 % (v/v) horse blood (Oxoid SR50).…”

Section: Colicine Typingmentioning

confidence: 99%

Comparison of four methods of differential typing of isolates ofShigella sonnei

Helgason

Old

1981

J. Hyg.

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“…Phage and bacteriocin typing of strains have been and are being used for epidemiological purposes (12,21,27). It has been observed in several species that strains belonging to specific phage types were more pathogenic or most often associated with a particular illness (27).…”

Section: Resultsmentioning

confidence: 99%

Laboratory and Wild-Type Klebsiella pneumoniae Strains Carrying Mannose-Inhibitable Adhesins and Receptors for Coliphages T3 and T7 Are More Pathogenic for Mice Than Are Strains Without Such Receptors

1983

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We have shown previously that Klebsiella pneumoniae receptors for coliphages T3 and T7 also mediate mannose-inhibitable adherence to human epithelial cells and protect bacteria from phagocytosis and intracellular killing by human polymorphonuclear cells. In this paper we analyze the possible role of such mannose-inhibitable adhesins and T3-T7 receptors (MIAT) in K. pneumoniae intraperitoneal pathogenicity for mice. We showed that intraperitoneal pathogenicity for mice of four different Klebsiella strains (one laboratory and three wild-type) that carry the MIAT was approximately 60-fold higher than that of four derivative strains that lost such receptors by spontaneous mutation. The MIAT could be repressed by Klebsiella phage AP3 lysogenic conversion. Two laboratory and two wild-type strains converted by phage AP3 were also approximately 60-fold less pathogenic for mice than parental strains and showed a pathogenicity level equal to that of the MIAT-negative mutants. Studies of protection in mice with anti-whole cell antisera showed that passive immunization against MIAT-positive cells was more protective than immunization against MIAT-negative cells. Studies of protection in mice by both active and passive immunization with lipopolysaccharide and purified outer membrane proteins have shown that the proteins are the most protective outer membrane components. Since it has been shown previously that the Klebsiella receptors for T3-T7 have a proteic component and that an outer membrane protein is missing in the strains resistant to T3-T7 (C. Pruzzo et al., in R. C. Berkely (ed.), Microbial Adhesion to Surfaces , 1980); the latter finding further supports the role of MIAT in the pathogenicity of Klebsiella for mice.

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Chapter II Bacteriocin Typing of Enterohacteriaceae

Cited by 2 publications

References 10 publications

Comparison of four methods of differential typing of isolates ofShigella sonnei

Comparison of four methods of differential typing of isolates ofShigella sonnei

Laboratory and Wild-Type Klebsiella pneumoniae Strains Carrying Mannose-Inhibitable Adhesins and Receptors for Coliphages T3 and T7 Are More Pathogenic for Mice Than Are Strains Without Such Receptors

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