Chapter 9 Mechanism of action of FSH in the ovary

Dahl, Kristine D.; Hsueh, Aaron J. W.

doi:10.1016/s0167-7306(08)60664-x

Cited by 3 publications

(5 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In mammals, FSH stimulates aromatase activity in the granulosa cells, thereby enhancing E production [59][60]. In salmon, as in mammals, cells in the thecal layer produce testosterone (T), which is converted to E by aromatase in granulosa layers during vitellogenesis [45,50,[56][57][58].…”

Section: Ovarymentioning

confidence: 99%

Localization of Two Gonadotropin Receptors in the Salmon Gonad by in Vitro Ligand Autoradiography1

Miwa

Yan

Swanson

1994

Biology of Reproduction

130

View full text Add to dashboard Cite

Receptors for two salmon gonadotropins, GTH I and GTH II, were localized by use of in vitro ligand autoradiography of coho salmon gonads at various stages of sexual maturation. The results in both sexes revealed the presence of two types of GTH receptors: type I (GTH-RI), which interacts with both GTHs, and type II (GTH-RII), which interacts specifically with GTH II. GTH-RI was found at all stages of spermatogenesis examined and was localized on cells that were most likely Sertoli cells; however, it could not be determined whether GTH-RI was also localized on Leydig cells. In contrast, GTH-RII was found only in Leydig cells in the testis from a spermiating fish. In the vitellogenic ovary, GTH-RI was localized in the thecal layer and intensely on granulosa cells; in the preovulatory follicle, in contrast, GTH-RI was found in the thecal layer and in interstitial connective tissue, but not in the granulosa layer. Among all the stages of oogenesis examined, only granulosa cells of the preovulatory follicle exhibited GTH-RII. The appearance of GTH-RII coincides well with the increase in plasma levels of GTH II that occurs during final oocyte maturation and spermiation in coho salmon. The nature, distribution, and timing of appearance of these two receptors can explain, at least in part, the results of previous studies on steroidogenic activities of the two GTHs. The present study also suggests the functional homology of salmon GTH I and GTH II to mammalian FSH and LH, respectively, during gonadal development.

show abstract

Section: Ovarymentioning

confidence: 99%

Localization of Two Gonadotropin Receptors in the Salmon Gonad by in Vitro Ligand Autoradiography1

Miwa

Yan

Swanson

1994

Biology of Reproduction

130

View full text Add to dashboard Cite

show abstract

“…Studies of the intracellular mechanisms by which FSH and LH regulate granulosa cell function have focused primarily upon the activation of adenylate cyclase with subsequent increases in cAMP and CAMPdependent protein kinase activity (Hsueh et al, 1985;Dahl and Hsueh, 1988;Amsterdam et al, 1989). How- ever, recent investigations have raised the possibility of alternate intracellular mechanisms, since stimulation with gonadotropins has been shown to stimulate PtdIns turnover (Davis et al, 1983(Davis et al, , 1984 and calcium influx in granulosa cells (Veldhuis and Klase, 1982;Veldhuis et al, 1984;Asem et al, 1987;Alila et al, 1989;Flares et al, 1990).…”

Section: Discussionmentioning

confidence: 99%

“…Since increased GPI labelling is accompanied by concomitant increases in hCG binding (Fig. 6), it is tempting to speculate that the observed augmentation in GPI content elicited by FSH might be another of the hormone associated events related with granulosa cell differentiation and LH/hCG receptor induction (Hsueh et al, 1985; Dahl and Hsueh, 1988). This hypothesis seems reasonable since ( 1) similar increases in glycosyl-phosphatidylinositol content have been reported after PHA induced increase in insulin receptor number in lymphocytes (Gaulton et al, 1988) or in stably transfected CHO cells overexpressing the human insulin receptor (Villalba et al, 1990); (2) treatment of cells with the protein kinase C-activating phorbol ester, an agent known to prevent granulosa cell differentiation (Welsh et al, 1984b;Shinohara et al, 1985); Ben-Ze'ev and ) is accompanied by a time-related decrease in GPI labelling (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The same morphological and biochemical changes elicited by FSH can be obtained after treatment of cells with membrane-permeable cAMP analogues or CAMPinducing agents (Richards et al, 1979;Knecht et al, 1981;Hunzicker-Dunn et al, 1984;Welsh et al, 1984a), and therefore generation of cAMP has been considered an important component of the mechanism whereby the gonadotropin regulates granulosa cells differentiation (Hsueh et al, 1985;Dahl and Hsueh, 1988;Amsterdam et al, 1989).…”

mentioning

confidence: 86%

“…cytoplasmic levels of cAMP and activation of protein kinase A (Hsueh et al, 1985;Dahl and Hsueh, 1988;Amsterdam et al, 1989). This process is accompanied by morphological cytodifferentiation , induction of steroidogenic enzymes and expression of receptors for the pituitary hormones LH and PRL (Hsueh et al, 1985;Dahl and Hsueh, 1988) or peptides and growth factors from diverse origin that could also be synthesized by ovarian cells Hsueh et al, 1985;Hsueh and Jones, 1981;Dahl and Hsueh, 1988;Adashi, 1990).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Follicle‐stimulating hormone and human chorionic gonadotropin induced changes in granulosa cell glycosyl‐phosphatidylinositol concentration

Fanjul

Marrero

Estévez

et al. 1993

Journal Cellular Physiology

View full text Add to dashboard Cite

In the present investigation, a hCG sensitive glycosyl-phosphatidylinositol (GPI) was isolated from cultured rat granulosa cells obtained from the ovaries of diethylstilbestrol (DES) implanted immature rats. The inositol-phosphoglycan (IPG) moiety of the GPI-lipid contains galactose, glucosamine, and myoinositol as demonstrated by metabolic labelling of granulosa cells for different time periods (5-96 h) with [3H]galactose, [3H]glucosamine, or [3H]myoinositol and treatment of the purified [3H]GPI with phosphatidylinositol-specific phospholipase C. Labelling equilibrium of the GPI-lipid was achieved after 24 h ([3H]galactose and [3H]myoinositol) or 72 h ([3H]glucosamine) incubation, whereas incorporation of other labelled carbohydrates tested ([3H]galactosamine, [3H]mannose, and [3H]sorbitol) was negligible throughout the time period studied. The glucosamine C-1 appears to be linked through a glycosidic bond to the myoinositol molecule of the IPG moiety as revealed by the generation of phosphatidylinositol (PtdIns) after nitrous acid deamination of dual labelled ([3H]glucosamine/[14C]palmitate or [3H]glucosamine/[14C]myristate) glycosyl-phosphatidylinositol. To investigate the fatty acid composition of the diacylglycerol (DAG) backbone of the GPI, granulosa cells were also labelled (5-72 hr) with [14C]linoleate, [3H]myristate, [3H]oleate, [3H]palmitate, or [3H]stearate and the radioactivity associated with the purified glycosyl-phosphatidylinositol determined. Incorporation of [3H]palmitate and [3H]myristate into the GPI-lipid peaked after 8 h and 24 h of labelling, respectively, and both fatty acids were partially released after PLA2 treatment of the dual labelled ([3H]glucosamine/[14C]palmitate or [3H]glucosamine/[14C]myristate) GPI. In parallel experiments no significant incorporation of labelled stearate, oleate, or linoleic acid into the DAG backbone of the glycosylphosphatidylinositol could be detected. Granulosa cells were also labelled with [3H]glucosamine in the presence of FSH (30 ng/ml), cholera toxin (1 microgram/ml), or the membrane permeable cAMP analog (but)2cAMP (1 mM). Time related increases in GPI-labelling were apparent after 48 h and reached a maximum level (3-, 5-, and 7-fold for FSH, CT, and (but)2cAMP, respectively) after 72 h in culture. In another set of experiments, granulosa cells were labelled for 72 h with [3H]glucosamine in the presence of (but)2cAMP (1 mM), TPA (10(-7) M), or combination thereof. The effect of treatment with the membrane permeable cAMP analog on GPI labelling was prevented in the presence of TPA, whereas no differences in [3H]GPI content could be observed in untreated granulosa cells or cells cultured in the presence of the protein kinase C-activating phorbol ester alone.(ABSTRACT TRUNCATED AT 400 WORDS)

show abstract

Inhibitory role of sex steroid in the regulation of ovarian follicle‐stimulating hormone receptors during pregnancy

Tsutsui

1992

J. Exp. Zool.

View full text Add to dashboard Cite

The present study was conducted to determine the role of sex steroids in the regulation of FSH receptors in pregnant rats. In the normal physiological condition, FSH bindings per unit ovarian weight (density of binding) and per 2 ovaries (total binding) increased during days 14-21 gestation. Scatchard plot analyses of the binding suggested that the increase in FSH binding was due to an increase in the number of FSH-binding sites. The plasma FSH concentration in pregnant rats was stable during the receptor change. In contrast, the plasma estradiol-17 beta concentration continuously increased from gestation day 14 to 21, and the testosterone level showed a large peak on gestation day 18. Estradiol-17 beta (one silastic plate containing 13 mg crystal)-implanted pregnant rats during 14-21 days of gestation induced significant decreases in the total FSH binding and ovarian weight on gestation day 21. Estradiol administration increased the plasma estradiol level 2.3-fold but did not change the FSH level. Testosterone or 5 alpha-dihydrotestosterone, a nonaromatizable androgen, did not influence the binding level under the same dose treatment. In contrast, continuous treatment with aminoglutethimide (2 plates containing 20 mg crystal), an inhibitor of adrenocortical steroidogenesis, for 7 days significantly increased the total FSH binding without a significant change in the ovarian weight. The plasma titers of estradiol and testosterone in pregnant rats treated with aminoglutethimide were reduced by 37% and 51%, respectively. Aminoglutethimide did not influence plasma FSH levels. These results suggest that circulating estradiol acts as a negative factor in the regulation of ovarian FSH receptors, at least during the second half of pregnancy. Other factor(s) that is (are) independent of sex steroids and FSH may contribute to FSH receptor induction.

show abstract

Chapter 9 Mechanism of action of FSH in the ovary

Cited by 3 publications

References 68 publications

Localization of Two Gonadotropin Receptors in the Salmon Gonad by in Vitro Ligand Autoradiography1

Localization of Two Gonadotropin Receptors in the Salmon Gonad by in Vitro Ligand Autoradiography1

Follicle‐stimulating hormone and human chorionic gonadotropin induced changes in granulosa cell glycosyl‐phosphatidylinositol concentration

Inhibitory role of sex steroid in the regulation of ovarian follicle‐stimulating hormone receptors during pregnancy

Contact Info

Product

Resources

About