Chapter 7 Cell Hybridization, Hybridomas, and Human Hybridomas

Shirahata, Sanetaka; Katakura, Yoshinori; Teruya, Kiichiro

doi:10.1016/s0091-679x(08)61575-7

Cited by 23 publications

(11 citation statements)

References 60 publications

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“…31 They introduced an HCN family channel (HCN1) into fibroblasts using a lentivirus to produce a stable expression of the HCN current. Then they injected HCN1-overexpressing fibroblasts into guinea-pig ventricles with polyethylene glycol, resulting in the creation of biological pacemakers.…”

Section: Gene and Cell Therapymentioning

confidence: 99%

Gene Therapy for Cardiac Arrhythmias

Donahue

Kikuchi

Sasano

2005

Trends in Cardiovascular Medicine

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Section: Gene and Cell Therapymentioning

confidence: 99%

Gene Therapy for Cardiac Arrhythmias

Donahue

Kikuchi

Sasano

2005

Trends in Cardiovascular Medicine

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“…These cells were fused with freshly isolated guinea-pig ventricular myocytes using polyethylene glycol 1500 (PEG). The PEG-induced membrane fusion events have served as a model system to create mouse and human hybridomas [65], to study eukaryotic cell-cell fusion events [41], and to deliver outward K + currents into myocytes [30]. In our experiments, PEG-induced fusion occurred almost instantaneously in vitro since, within 3 min, the HCN1-fibroblasts fused with ventricular myocytes as verified by the sudden introduction of Calcein-AM fluorescence into the myocytes.…”

Section: Creation Of a Biological Pacemaker By Cell Fusionmentioning

confidence: 52%

Creation of a biological pacemaker by gene- or cell-based approaches

Marbán

Cho

2007

Med Bio Eng Comput

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Cardiac rhythm-associated disorders are caused by mal-functions of impulse generation and conduction. Present therapies for the impulse generation span a wide array of approaches but remain largely palliative. The progress in the understanding of the biology of the diseases with related biological tools beckons for new approaches to provide better alternatives to the present routine. Here, we review the current state of the art in gene- and cell-based approaches to correct cardiac rhythm disturbances. These include genetic suppression of an ionic current, stem cell therapies, adult somatic cell-fusion approach, novel synthetic pacemaker channel, and creating a self-contained pacemaker activity in non-excitable cells. We then conclude by discussing advantages and disadvantages of the new possibilities.

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“…There have been made several attempts to improve this method and to increase the yield of hybridomas that produce the antibody of interest (Browne and Al-Rubeai, 2007;Shirahata et al, 1998). But most of these techniques were not applicable as routine method or were just quantitative.…”

Section: Discussionmentioning

confidence: 99%