Chapter 5 Thermostable Enzymes and Their Industrial Applications

Kumar, S Pradeep; Arumugam, Nanthakumar; Permaul, Kugenthiren; Singh, Suren

doi:10.1201/9781315367880-6

Cited by 7 publications

(3 citation statements)

References 237 publications

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“…These parameters are important for the industrial applications of enzymes. 4 Thermophiles (optimal growth above 50°C) and hyperthermophiles (optimal growth above 80°C) are among the most studied microorganisms in extreme environments. 5,6 Their natural biotopes vary and can be terrestrial (geothermal springs, desert and volcanic soils, oil drills, and compost) or marine (deep hydrothermal mountains).…”

Section: Introductionmentioning

confidence: 99%

Characterization and Identification of Thermophilic Bacteria Isolated from Different Sites Located in Al-Jouf Region, Saudi Arabia

Saidan,

Jarboui,

Alsharari

et al. 2024

J. Pure Appl. Microbiol.

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This study aims to investigate the morphological and biochemical characteristics of thermophilic bacteria isolated from thermophilic biotopes in the Al-Jouf region of Saudi Arabia for the first time. Additionally, the production of thermostable bacterial enzymes (proteases, amylases, cellulases, gelatinases, and lipases) was investigated. Six main bacterial species were identified via 16S rRNA sequencing and phylogenetic analysis. Twenty water and soil samples were collected from several thermophilic sites in the Dumat Al-Jandal and Al-Qurayyat cities. In total, 28 bacterial strains were isolated and biochemically characterized. Most isolated strains showed high protease and amylase production. 46 and 28% of isolated strains showed the production of cellulase and gelatinase, respectively. However, lipase production was not observed in any isolated bacteria. Four Bacillus licheniformis and two Bacillus subtilis strains exhibited high GC content (55%). Our findings suggest thermophilic bacteria as a source of thermostable enzymes for pharmaceutical and industrial applications.

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Section: Introductionmentioning

confidence: 99%

Characterization and Identification of Thermophilic Bacteria Isolated from Different Sites Located in Al-Jouf Region, Saudi Arabia

Saidan,

Jarboui,

Alsharari

et al. 2024

J. Pure Appl. Microbiol.

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“…Enzymes have continued to gain prominence in food processing over traditional chemical-and mechanical-based methods, as they are eco-friendly and have been shown to increase food nutritional value, with lesser energy input requirement [Kumar et al, 2016]. Various carbohydrases have since been produced commercially for their applications in fruit juice processing, as the potential of novel enzyme sources in enhancing customer desirability is continually being investigated.…”

Section: Introductionmentioning

confidence: 99%

Pear Juice Clarification Using Polygalacturonase from <i>Beauveria bassiana</i>: Effects on Rheological, Antioxidant and Quality Properties

Amobonye¹,

Bhagwat²,

Ruzengwe³

et al. 2022

Pol. J. Food Nutr. Sci.

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“…Numerous commercial proteases, especially isolated from microorganisms, are used in various industrial and analytical processes, such as protein analysis, feed and food biotechnology, pharmaceutical and cosmetic preparations, and cleaning processes [ 3 , 4 , 5 ]. For example, they have major applications in detergent formulations, cheese-making, baking, meat tenderization, and leather industries [ 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Production and Characterization of an Extracellular Acid Protease from Thermophilic Brevibacillus sp. OA30 Isolated from an Algerian Hot Spring

Gomri¹,

Rico-Díaz

Escuder-Rodríguez

et al. 2018

Microorganisms

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Proteases have numerous biotechnological applications and the bioprospection for newly-thermostable proteases from the great biodiversity of thermophilic microorganisms inhabiting hot environments, such as geothermal sources, aims to discover more effective enzymes for processes at higher temperatures. We report in this paper the production and the characterization of a purified acid protease from strain OA30, a moderate thermophilic bacterium isolated from an Algerian hot spring. Phenotypic and genotypic study of strain OA30 was followed by the production of the extracellular protease in a physiologically-optimized medium. Strain OA30 showed multiple extracellular proteolytic enzymes and protease 32-F38 was purified by chromatographic methods and its biochemical characteristics were studied. Strain OA30 was affiliated with Brevibacillus thermoruber species. Protease 32-F38 had an estimated molecular weight of 64.6 kDa and was optimally active at 50 °C. It showed a great thermostability after 240 min and its optimum pH was 6.0. Protease 32-F38 was highly stable in the presence of different detergents and solvents and was inhibited by metalloprotease inhibitors. The results of this work suggest that protease 32-F38 might have interesting biotechnological applications.

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Chapter 5 Thermostable Enzymes and Their Industrial Applications

Cited by 7 publications

References 237 publications

Characterization and Identification of Thermophilic Bacteria Isolated from Different Sites Located in Al-Jouf Region, Saudi Arabia

Characterization and Identification of Thermophilic Bacteria Isolated from Different Sites Located in Al-Jouf Region, Saudi Arabia

Pear Juice Clarification Using Polygalacturonase from <i>Beauveria bassiana</i>: Effects on Rheological, Antioxidant and Quality Properties

Production and Characterization of an Extracellular Acid Protease from Thermophilic Brevibacillus sp. OA30 Isolated from an Algerian Hot Spring

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