Chapter 5 Neuroendocrine and epithelial antigens in SCLC

Leij, Lou de; Berendsen, Hh; Spakman, H.; Haar, A. ter; The, H. S. Goei

doi:10.1016/s0169-5002(88)80007-2

Cited by 15 publications

(4 citation statements)

References 5 publications

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“…MAbs MM104 (36), MOC31 (17), and 311-1K1 (25) were kindly supplied by L. de Leij (University of Groningen, Groningen, The Netherlands). MAbs KS1/4 (11), MM104 (23), and 2G8 (unpublished data) were provided by R. Reisfeld (The Scripps Research Institute, La Jolla, Calif.), S. Alberti (University of Naples, Naples, Italy), and G. Riethmüller (University of Munich, Munich, Germany), respectively.…”

Section: Methodsmentioning

confidence: 99%

Epidermal Growth Factor-Like Repeats Mediate Lateral and Reciprocal Interactions of Ep-CAM Molecules in Homophilic Adhesions

Balzar

Bruijn

Rees-Bakker

et al. 2001

Molecular and Cellular Biology

160

157

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Ep-CAM is a new type of cell adhesion molecule (CAM) which does not structurally resemble the members of the four major families (cadherins, integrins, selectins, and CAMs of the immunoglobulin superfamily) and mediates Ca 2؉ -independent, homophilic adhesions. The extracellular domain of Ep-CAM consists of a cysteine-rich region, containing two type II epidermal growth factor (EGF)-like repeats, followed by a cysteine-poor region. We generated mutated Ep-CAM forms with various deletions in the extracellular domain. These deletion mutants, together with monoclonal antibodies recognizing different epitopes in the extracellular domain, were used to investigate the role of the EGF-like repeats in the formation of intercellular contacts mediated by Ep-CAM molecules. We established that both EGF-like repeats are required for the formation of Ep-CAM-mediated homophilic adhesions, including the accumulation of Ep-CAM molecules at the cell-cell boundaries, and the anchorage of the Ep-CAM adhesion complex to F-actin via ␣-actinin. Deletion of either EGF-like repeat was sufficient to inhibit the adhesion properties of the molecule. The first EGF-like repeat of Ep-CAM is required for reciprocal interactions between Ep-CAM molecules on adjacent cells, as was demonstrated with blocking antibodies. The second EGF-like repeat was mainly required for lateral interactions between Ep-CAM molecules. Lateral interactions between Ep-CAM molecules result in the formation of tetramers, which might be the first and necessary step in the formation of Ep-CAM-mediated intercellular contacts.

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Section: Methodsmentioning

confidence: 99%

Epidermal Growth Factor-Like Repeats Mediate Lateral and Reciprocal Interactions of Ep-CAM Molecules in Homophilic Adhesions

Balzar

Bruijn

Rees-Bakker

et al. 2001

Molecular and Cellular Biology

160

157

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“…Although the cell line had been derived from a SCLC xenograft with obvious SCLC pathology it was important to confirm that the biochemical and antigen features were consistent with SCLC origin. Levels of the biochemical markers L-3,4-dihydroxyphenylalanine decarboxylase and creatine kinase in WX322 cells were typical of SCLC Gazdar et al, 1985) as was the presence of both epithelial and neuroendocrine antigens (de Leij et al, 1988). When WX322 cells were grown in agar, colony formation was inhibited by concentrations of IFN less than 10 IU ml' while concentrations of greater than 104 IU ml1 were needed to produce comparable effects in the NCI-H69 cell line.…”

Section: Discussionmentioning

confidence: 96%

Characterisation and properties of a small cell lung cancer cell line and xenograft WX322 with marked sensitivity to alpha-interferon

Langdon

Gj²,

Anderson³

et al. 1991

Br J Cancer

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“…To explore the potential of PCI in activating the cytotoxic potential of an IT, the immunoconjugate MOC31-gelonin was selected as a model IT [35]. The monoclonal antibody MOC31 recognises and targets human pancarcinoma-associated epithelial glycoprotein 2, an antigen expressed on nearly all types of carcinoma cells [36]. Epithelial glycoprotein 2 is also recognised as the epithelial cell adhesion molecule or 17-1A [37].…”

Section: Pci-based Delivery Of Proteinsmentioning

confidence: 99%

Photochemical Internalisation: A Novel Drug Delivery System

Selbo

Høgset²,

Prasmickaite

et al. 2002

Tumor Biol

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The present report reviews a number of recently published papers on a novel technology for the cytosolic delivery of macromolecules named photochemical internalisation (PCI). PCI is based upon the light activation of a drug (a photosensitiser) specifically located in the membrane of endocytic vesicles. Light which is absorbed by the photosensitiser induces the formation of reactive oxygen species, of which singlet oxygen (¹O₂) is the predominant form. Singlet oxygen oxidises biomolecules in the membranes of endosomes and lysosomes, resulting in a subsequent release of the contents of these compartments into the cytosol. Photosensitisers have a higher affinity for tumour tissues than for most normal tissues and are used in photodynamic therapy of various types of cancers. We have taken advantage of the PCI strategy to enhance the delivery of a variety of macromolecules, including ribosome-inactivating toxins, an immunotoxin, horse radish peroxidase, a ras peptide, RNA, oligonucleotides and protein encoding DNA, to the cytosol. Normally, a major intracellular barrier to the application of therapeutically interesting peptides and proteins or the application of DNA and RNA in gene therapy is the degradation of the macromolecules in the endocytic vesicles after uptake by endocytosis. Therefore, a photochemically induced rupture of endocytic vesicles and the subsequent cytosolic release of the macromolecules aids these molecules in escaping attack by the lysosomal hydrolases, thereby maintaining their biological activity. Thus, PCI represents a novel principle for the cytosolic delivery of biologically active macromolecules which overcomes the pivotal intracellular barrier of endosomes and lysosomes. In addition to being utilised as a new site-specific cancer therapy method, PCI can also be applied as a research tool for macromolecule delivery both in vitro and in vivo.

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Chapter 5 Neuroendocrine and epithelial antigens in SCLC

Cited by 15 publications

References 5 publications

Epidermal Growth Factor-Like Repeats Mediate Lateral and Reciprocal Interactions of Ep-CAM Molecules in Homophilic Adhesions

Epidermal Growth Factor-Like Repeats Mediate Lateral and Reciprocal Interactions of Ep-CAM Molecules in Homophilic Adhesions

Characterisation and properties of a small cell lung cancer cell line and xenograft WX322 with marked sensitivity to alpha-interferon

Photochemical Internalisation: A Novel Drug Delivery System

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