Chapter 4 Laser ablation for inductively coupled plasma-mass spectrometry

Günther, Detlef; Mermet, Jean‐Michel

doi:10.1016/s0166-526x(00)34006-5

Cited by 12 publications

(13 citation statements)

References 230 publications

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“…Schematic of LA tem at the Ghent University. A general description of the LA systems can be found in [13]. For the scope of this paper, it is worth noticing that the ablation cell is mounted on a stage that allows translation, software-controlled, in all three spatial directions and that the cell is directly monitored by a camera connected to the same PC, making it easy to change the ablation site on a gel.…”

Section: Laser Ablationmentioning

confidence: 99%

“…A high 106 Ag 1 signal, from the silver-staining, proves that the ablation occurs at the site of the protein. 13 C 1 and 23 Na 1 are good indicators of the layer being ablated: gel or backing plastic. The density of these elements is lower in the backing plastic, indicating that the whole gel thickness (500 mm) has been ablated.…”

Section: Ablation Parameters and Lod With Page-la-icp-drc-msmentioning

confidence: 99%

“…The peak at 9 cm is probably due to the partial decomposition of selenocysteine into low-molecularmass Se compounds and the peak at 3 cm comes from high-molecular-mass compounds that were too large to leave the stacking gel to the separation gel. The peaks at 4.9 and 7.7 cm are artifacts, due to the opening of the cell to change the gel portions; a correction with 13 C as internal reference was not successful in solving this problem. In order to determine the distribution of Se in a protein band, the GSH-Px band was ablated in the perpendicular direction, in the width of the gel lane (data not shown).…”

Section: -De: Gsh-px In Red Blood Cellsmentioning

confidence: 99%

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Detection of metals in proteins by means of polyacrylamide gel electrophoresis and laser ablation‐inductively coupled plasma‐mass spectrometry: Application to selenium

et al. 2003

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The capabilities of laser ablation-inductively coupled plasma-mass spectrometry for the detection of trace elements in a gel after gel electrophoresis were systematically studied. Figures of merit, such as limit of detection, linearity, and repeatability, were evaluated for various elements (Li, V, Cr, Mn, Ni, Cu, Zn, As, Se, Mo, Pd, Ag, Cd, Pt, Tl, Pb). Two ablation strategies were followed: single hole drilling, relevant for ablation of spots after two-dimensional (2-D) separations, and ablation with translation, i.e., on a line, relevant for one-dimensional (1-D) separations. This technique was applied to the detection of selenoproteins in red blood cells extracts after a 1-D separation (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) and the detection of selenium-containing proteins in yeast after 2-D electrophoresis (2-DE). The detection procedure was further improved by using the dynamic reaction cell technology, which allowed the removal of the Ar 2 interference and hence the use of the most abundant Se isotope, 80 Se. Reaction gases were compared (methane, carbon monoxide, ammonia, oxygen and the combination of argon (collision gas) and hydrogen (reaction gas)). In each instance, the reaction cell parameters were optimized in order to obtain the lowest detection limit for Se (as 80 Se 16 O 1 with O 2 as the reaction gas). Carbon monoxide was found to offer the best performance. The detection limit with the use of DRC and He as transport gas was 0.07 mg Se g 21 gel with single hole drilling and 0.15 mg Se g 21 gel for ablation with translation.

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Section: Laser Ablationmentioning

confidence: 99%

Section: Ablation Parameters and Lod With Page-la-icp-drc-msmentioning

confidence: 99%

Section: -De: Gsh-px In Red Blood Cellsmentioning

confidence: 99%

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Detection of metals in proteins by means of polyacrylamide gel electrophoresis and laser ablation‐inductively coupled plasma‐mass spectrometry: Application to selenium

et al. 2003

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“…As its name implies, LA involves the removal of a minute amount of sample by a laser beam. Figure 6 shows a typical LA system, which consists of a laser, lens to focus the beam onto the sample surface, ablation cell in which the sample is placed, and a transfer line through which the aerosol generated by LA is carried by a gas flow into the ICP (Günther & Mermet, 2000). The suitability of the system for sample introduction in ICP-MS depends on the laser wavelength, pulse energy, pulse-to-pulse stability, pulse frequency, pulse duration, laser beam profile and ablation spot size (Vogt & Latkoczy, 2005).…”

Section: Laser Ablation (La)mentioning

confidence: 99%

Environmental analysis by inductively coupled plasma mass spectrometry

Beauchemin

2009

Mass Spectrometry Reviews

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This article reviews the numerous ways in which inductively coupled plasma mass spectrometry has been used for the analysis of environmental samples since it was commercially introduced in 1983. Its multielemental isotopic capability, high sensitivity and wide linear dynamic range makes it ideally suited for environmental analysis. Provided that some care is taken during sample preparation and that appropriate calibration strategies are used to circumvent non-spectroscopic interferences, the technique is readily applicable to the analysis of a wide variety of environmental samples (natural waters, soils, rocks, sediments, vegetation, etc.), using quadrupole, time-of-flight or double-focusing sector-field mass spectrometers. In cases where spectroscopic interferences arising from the sample matrix cannot be resolved, then separation methods can be implemented either on-or off-line, which can simultaneously allow analyte preconcentration, thus further decreasing the already low detection limits that are achievable. In most cases, the blank, prepared by following the same steps as for the sample but without the sample, limits the ultimate detection limits that can be reached.

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“…Another well-known disadvantage to LA is elemental fractionation. Fractionation is a dynamic process that includes the effects of the ablation, sampling, transport and ionization and is defined as producing ablation products that are not stoichiometrically representative of the sample composition [23]. Several studies describing fractionation as well as some approaches to minimize it have been reported.…”

Section: Tatiana Trejos · Shirly Montero · José R Almirallmentioning

confidence: 99%

Analysis and comparison of glass fragments by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) and ICP-MS

Trejos

Montero

Almirall

2003

Analytical and Bioanalytical Chemistry

124

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The discrimination potential of Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) is compared with previously reported solution ICP-MS methods using external calibration (EC) with internal standardization and a newly reported solution isotope dilution (ID) method for the analysis of two different glass populations. A total of 91 different glass samples were used for the comparison study; refractive index and elemental composition were measured by the techniques mentioned above. One set consisted of 45 headlamps taken from a variety of automobiles that represents a range of 20 years of manufacturing dates. A second set consisted of 46 automotive glasses (side windows, rear windows, and windshields) representing casework glass from different vehicle manufacturers over several years. The element menu for the LA-ICP-MS and EC-ICP-MS methods include Mg, Al, Ca, Mn, Ce, Ti, Zr, Sb, Ga, Ba, Rb, Sm, Sr, Hf, La, and Pb. The ID method was limited to the analysis of two isotopes each of Mg, Sr, Zr, Sb, Ba, Sm, Hf, and Pb. Laser ablation analyses were performed with a Q switched Nd:YAG, 266 nm, 6 mJ output energy laser. The laser was used in depth profile mode while sampling using a 50 microm spot size for 50 sec at 10 Hz (500 shots). The typical bias for the analysis of NIST 612 by LA-ICP-MS was less than 5% in all cases and typically better than 5% for most isotopes. The precision for the vast majority of the element menu was determined generally less than 10% for all the methods when NIST 612 was measured (40 microg x g(-1)). Method detection limits (MDL) for the EC and LA-ICP-MS methods were similar and generally reported as less than 1 microg x g(-1) for the analysis of NIST 612. While the solution sample introduction methods using EC and ID presented excellent sensitivity and precision, these methods have the disadvantages of destroying the sample, and also involve complex sample preparation. The laser ablation method was simpler, faster, and produced comparable discrimination to the EC-ICP-MS and ID-ICP-MS. LA-ICP-MS can offer an excellent alternative to solution analysis of glass in forensic casework samples.

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Chapter 4 Laser ablation for inductively coupled plasma-mass spectrometry

Cited by 12 publications

References 230 publications

Detection of metals in proteins by means of polyacrylamide gel electrophoresis and laser ablation‐inductively coupled plasma‐mass spectrometry: Application to selenium

Detection of metals in proteins by means of polyacrylamide gel electrophoresis and laser ablation‐inductively coupled plasma‐mass spectrometry: Application to selenium

Environmental analysis by inductively coupled plasma mass spectrometry

Analysis and comparison of glass fragments by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) and ICP-MS

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