Chapter 4.6. Gel chromatography

Berek, Dušan; Marcinka, K

doi:10.1016/s0167-7306(08)60219-7

Cited by 9 publications

(6 citation statements)

References 130 publications

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“…The aim of SEC is to discriminate macromolecules of distinct sizes dissolved in appropriate solvent – not only to determine their molecular characteristics but also to remove undesired admixtures. There exist several monographs, chapters in books and review articles on this subject, for example 3–33. Most of them contain numerous quotations on articles devoted to theory, instrumentation, and applications of SEC.…”

Section: Macromolecular Substances Polymersmentioning

confidence: 99%

“…The direct relationship between packing pore size distribution determined by mercury porosimetry and experimental dependences of the type log M versus V R and log M [η] versus V R could not be clearly established 54. This is one of the reasons why many theoretical analyses of SEC concentrated mostly on explanation and evaluation of band‐broadening effects, for review see for example 14, 16, 34.…”

Section: Retention Mechanisms In Polymer Hplcmentioning

confidence: 99%

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Size exclusion chromatography – A blessing and a curse of science and technology of synthetic polymers

Berek

2010

J of Separation Science

151

126

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Size exclusion chromatography, SEC is one of the most popular methods for the separation of different kinds of macromolecules. This critical review gives concise information about macromolecules and their behavior in solution, basic understanding about principles, instrumentation, and application possibilities of SEC, and more in detail discusses drawbacks and pitfalls of the method with the emphasis on synthetic polymers. Selected practical advices are included to help enhance the quality of SEC results.

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Section: Macromolecular Substances Polymersmentioning

confidence: 99%

Section: Retention Mechanisms In Polymer Hplcmentioning

confidence: 99%

Size exclusion chromatography – A blessing and a curse of science and technology of synthetic polymers

Berek

2010

J of Separation Science

151

126

View full text Add to dashboard Cite

show abstract

“…The objective of MW determinations requires pure, stable, and available polymers as standards, usually proteins, enzymes, or viruses that have been identified and are now commercially available [143]. The objective of MW determinations requires pure, stable, and available polymers as standards, usually proteins, enzymes, or viruses that have been identified and are now commercially available [143].…”

Section: H Size-exclusion Chromatographymentioning

confidence: 99%

The Trails of Research in Chromatography

Wixom

2010

Chromatography

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“…Unfortunately, perfect fractionation is impossible, due to secondary fractionation mechanisms and band broadening (BB). Secondary fractionations result from physicochemical interactions between the analyzed polymer, the solvent, and the column packing [2], and will not be further discussed. BB is mainly due to axial dispersion in the fractionation columns, while other minor sources include column-end effects, finite injection volumes, finite detection cell volumes, and laminar flow profiles in the capillaries [3,4].…”

Section: Introductionmentioning

confidence: 99%