Chapter 27 Three-dimensional imaging of extracellular matrix and extracellular matrix-cell interactions

Sl, Voytik-Harbin; Rajwa, Bartek; Jp, Robinson

doi:10.1016/s0091-679x(01)63031-0

Cited by 50 publications

(52 citation statements)

References 23 publications

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“…The probe has a low binding affinity and does not affect mechanical properties of the tissue, is small, and can thus readily diffuse into the tissue. Collagen fibers and bundles can also be visualized in tissue without labeling agents or fixative as a result of its intrinsic properties such as bifringence under polarized light microscopy (Finlay et al 1995;Hilbert et al 1996), auto-fluorescence (Voytik-Harbin et al 2001), and second harmonic generation (SHG) (Campagnola and Loew 2003;Boulesteix et al 2006). Techniques for 3D visualization of collagen without specific probes include auto-fluorescence, confocal reflection microscopy (Roeder et al 2002), and second harmonic generation using femtosecond pulsed infrared excitation (Boulesteix et al 2006).…”

Section: Collagen Visualizationmentioning

confidence: 99%

Experimental investigation of collagen waviness and orientation in the arterial adventitia using confocal laser scanning microscopy

Rezakhaniha

Agianniotis

Schrauwen

et al. 2011

Biomech Model Mechanobiol

895

786

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Mechanical properties of the adventitia are largely determined by the organization of collagen fibers. Measurements on the waviness and orientation of collagen, particularly at the zero-stress state, are necessary to relate the structural organization of collagen to the mechanical response of the adventitia. Using the fluorescence collagen marker CNA38-OG488 and confocal laser scanning microscopy, we imaged collagen fibers in the adventitia of rabbit common carotid arteries ex vivo. The arteries were cut open along their longitudinal axes to get the zero-stress state. We used semi-manual and automatic techniques to measure parameters related to the waviness and orientation of fibers. Our results showed that the straightness parameter (defined as the ratio between the distances of endpoints of a fiber to its length) was distributed with a beta distribution (mean value 0.72, variance 0.028) and did not depend on the mean angle orientation of fibers. Local angular density distributions revealed four axially symmetric families of fibers with mean directions of 0 • , 90 • , 43 • and −43 • , with respect to the axial direction of the artery, and corresponding circular standard deviations of 40 • , 47 • , 37 • and 37 • . The distribution of local orientations was shifted to the circumferential direction when measured in arteries at the zero-load state (intact), as compared to arteries at the zero-stress state (cutopen). Information on collagen fiber waviness and orientation, such as obtained in this study, could be used to develop structural models of the adventitia, providing better means for analyzing and understanding the mechanical properties of vascular wall.

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Section: Collagen Visualizationmentioning

confidence: 99%

Experimental investigation of collagen waviness and orientation in the arterial adventitia using confocal laser scanning microscopy

Rezakhaniha

Agianniotis

Schrauwen

et al. 2011

Biomech Model Mechanobiol

895

786

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“…Scanning confocal microscopy was utilized to visualize unstained collagen fibers as previously described (Brightman, 2000, Voytik-Harbin, 2001. Briefly, laser light with a wave length of 488 nm was illuminated onto the samples, and the reflected light was detected with a photomultiplier tube (PMT) using a blue reflection filter on a Leica TCS SL confocal microscopy system.…”

Section: Confocal Microscopymentioning

confidence: 99%

Microfluidic alignment of collagen fibers for in vitro cell culture

Lee

Lin

Moon

et al. 2006

Biomed Microdevices

203

170

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Three dimensional gels of aligned collagen fibers were patterned in vitro using microfluidic channels. Collagen fiber orientation plays an important role in cell signaling for many tissues in vivo, but alignment has been difficult to realize in vitro. For microfluidic collagen fiber alignment, collagen solution was allowed to polymerize inside polydimethyl siloxane (PDMS) channels ranging from 10-400 microm in width. Collagen fiber orientation increased with smaller channel width, averaging 12+/-6 degrees from parallel for channels between 10 and 100 microm in width. In these channels 20-40% of the fibers were within 5 degrees of the channel axis. Bovine aortic endothelial cells expressing GFP-tubulin were cultured on aligned collagen substrate and found to stretch in the direction of the fibers. The use of artificially aligned collagen gels could be applied to the study of cell movement, signaling, growth, and differentiation.

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“…Collagen fibres typically have a diameter in between 10-500 nm (Ushiki, 2002). Several successful attempts have been performed to study ECM remodelling in time by using confocal reflection microscopy in 3D fibrin lattices and fibroblast populated collagen lattices (Hartmann et al, 2006;Brightman et al, 2000;Voytik-Harbin et al, 2001;Wolf and Friedl, 2005). However, with confocal reflection microscopy alone it is not possible to distinguish between different constituents, whereas with multiple fluorescent labels in combination with laser scanning microscopy this is possible.…”

Section: Discussionmentioning

confidence: 99%

“…Techniques for 3D visualisation of collagen without the use of specific probes include autofluorescence (Voytik-Harbin et al, 2001;Zipfel et al, 2003), confocal reflection microscopy (Hartmann et al, 2006;Voytik-Harbin et al, 2001;Brightman et al, 2000;Wolf and Friedl, 2005) and second harmonic generation (SHG) using femtosecond pulsed infrared excitation (Cox et al, 2003;Campagnola et al, 2002;Zoumi et al, 2002;Konig and Riemann, 2003). Several tissue constituents possess intrinsic autofluorescence properties, enabling them to be visualised using confocal or multi-photon microscopy without the use of added probes.…”

Section: Introductionmentioning

confidence: 99%

High resolution imaging of collagen organisation and synthesis using a versatile collagen specific probe

Boerboom

Krahn

Megens

et al. 2007

Journal of Structural Biology

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Chapter 27 Three-dimensional imaging of extracellular matrix and extracellular matrix-cell interactions

Cited by 50 publications

References 23 publications

Experimental investigation of collagen waviness and orientation in the arterial adventitia using confocal laser scanning microscopy

Experimental investigation of collagen waviness and orientation in the arterial adventitia using confocal laser scanning microscopy

Microfluidic alignment of collagen fibers for in vitro cell culture

High resolution imaging of collagen organisation and synthesis using a versatile collagen specific probe

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