Chapter 23 High-Gradient Magnetic Cell Sorting

Radbruch, Andreas; Mechtold, Birgit; Thiel, Andreas; Miltenyi, Stefan; Pflüger, E.

doi:10.1016/s0091-679x(08)61086-9

Cited by 87 publications

(45 citation statements)

References 10 publications

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“…Nevertheless, if the saturation magnetization is neglected and the maximum magnetic field gradient can be applied, the maximal F M that can be reached in biological systems is in the order of 1.5 Â 10 À4 N for 100 mm particles. Even though this force can possibly exceed the centrifugal force, it has been applied in biotechnology mostly on a small scale for the concentration and separation of cells (Comella et al, 2001;Cullison and Jaykus, 2002;McCloskey et al, 2003;Melville et al, 1975;Owen, 1978;Radbruch et al, 1994;Zborowski et al, 2003) and the purification of molecules by their adsorption onto magnetic adsorbents followed by recovery of the magnetic adsorbents (Heeboll-Nielsen et al, 2003;Hubbuch and Thomas, 2003).…”

Section: Magnetic Forcementioning

confidence: 99%

Strategy for selection of methods for separation of bioparticles from particle mixtures

Hee

Hoeben

Lans

et al. 2006

Biotech & Bioengineering

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The desired product of bioprocesses is often produced in particulate form, either as an inclusion body (IB) or as a crystal. Particle harvesting is then a crucial and attractive form of product recovery. Because the liquid phase often contains other bioparticles, such as cell debris, whole cells, particulate biocatalysts or particulate by-products, the recovery of product particles is a complex process. In most cases, the particulate product is purified using selective solubilization or extraction. However, if selective particle recovery is possible, the already high purity of the particles makes this downstream process more favorable. This work gives an overview of typical bioparticle mixtures that are encountered in industrial biotechnology and the various driving forces that may be used for particle-particle separation, such as the centrifugal force, the magnetic force, the electric force, and forces related to interfaces. By coupling these driving forces to the resisting forces, the limitations of using these driving forces with respect to particle size are calculated. It shows that centrifugation is not a general solution for particle-particle separation in biotechnology because the particle sizes of product and contaminating particles are often very small, thus, causing their settling velocities to be too low for efficient separation by centrifugation. Examples of such separation problems are the recovery of IBs or virus-like particles (VLPs) from (microbial) cell debris. In these cases, separation processes that use electrical forces or fluid-fluid interfaces show to have a large potential for particle-particle separation. These methods are not yet commonly applied for large-scale particle-particle separation in biotechnology and more research is required on the separation techniques and on particle characterization to facilitate successful application of these methods in industry.

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Section: Magnetic Forcementioning

confidence: 99%

Strategy for selection of methods for separation of bioparticles from particle mixtures

Hee

Hoeben

Lans

et al. 2006

Biotech & Bioengineering

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“…The unique feature of the magnetite particle is its reaction to magnetic force. Magnetite particles have been used for cell sorting, as high magnetic flux density attracts magnetically labeled cells (Miltenyi et al, 1990;Radbruch et al, 1994;Moore et al, 1998;Lewin et al, 2000). We previously developed magnetite cationic liposomes (MCLs), which contain 10-nm magnetite nanoparticles, in order to improve accumulation of magnetite nanoparticles in target cells, due to electrostatic interaction between MCLs and the cell membrane (Shinkai et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Incorporation of Capillary-Like Structures into Dermal Cell Sheets Constructed by Magnetic Force-Based Tissue Engineering

Ino

Ito

Kumazawa

et al. 2007

J. Chem. Eng. Japan / JCEJ

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One of the major challenges in tissue engineering remains the construction of vascularized 3D transplants in vitro.We recently proposed novel technologies, termed "magnetic force-based tissue engineering" (Mag-TE), to establish three-dimensional (3D) tissues without using scaffolds. Magnetite cationic liposomes (MCLs), which contain 10-nm magnetite nanoparticles in order to improve accumulation of magnetite nanoparticles in target cells, were used to magnetically label normal human dermal fibroblasts (NHDFs). Magnetically labeled NHDFs were seeded onto ultralow-attachment plates. When a magnet was placed under the plate, cells accumulate on the bottom of the well. After a 24-h-incubation period, the cells form a sheet-like structure, which contains the major dermal extracellular matrix (ECM) components (fibronectin and type I collagen) within the NHDF sheet. Human umbilical vein endothelial cells (HUVECs) were co-cultured with NHDF sheets by two methods: HUVECs and NHDFs were mixed and then allowed to form cell sheets by Mag-TE; or NHDF sheets were constructed by Mag-TE and HUVECs were subsequently seeded onto NHDF sheets. These methods gave tube-like formation of HAECs, resembling early capillaries, within or on the surface NHDF sheets after short-term 3D co-culture, thus suggesting that Mag-TE may be useful for constructing 3D-tissue involving capillaries.

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“…These microspheres can be attached to various bioanalytes or cells with high selectivity by functionalizing the bead surfaces with suitable bioconjugates, making it possible to manipulate the analyte-bead amalgam with external magnetic fields. [2][3][4] Magnetic microspheres have been used in microfluidic micro-total-analytical-systems devices, which are tools for biological and biochemical analyses. 5 These beads are easily recovered by using high gradient magnetic separation.…”

mentioning

confidence: 99%

Single magnetic particle dynamics in a microchannel

Sinha

Ganguly

et al. 2007

Physics of Fluids

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Functionalized magnetic particles are used in micrototal analysis systems since they act as magnetically steered mobile substrates in microfluidic channels, and can be collected for bioanalytical processing. Here, we examine the motion of magnetic microbeads in a microfluidic flow under the influence of a nonuniform external magnetic field and characterize their collection in terms of the magnetic field strength, particle size, magnetic susceptibility, host fluid velocity and viscosity, and the characteristic length scale. We show that the collection efficiency of a magnetic collector depends upon two dimensionless numbers that compare the magnetic and particle drag forces.

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Chapter 23 High-Gradient Magnetic Cell Sorting

Cited by 87 publications

References 10 publications

Strategy for selection of methods for separation of bioparticles from particle mixtures

Strategy for selection of methods for separation of bioparticles from particle mixtures

Incorporation of Capillary-Like Structures into Dermal Cell Sheets Constructed by Magnetic Force-Based Tissue Engineering

Single magnetic particle dynamics in a microchannel

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