Chapter 18 Methods in the Cellular and Molecular Biology of Paramecium

Hanson, Earl D.

doi:10.1016/s0091-679x(08)60457-4

Cited by 11 publications

(3 citation statements)

References 192 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Applications of the technique described here to selection protocols following mutagenesis or the generation of cortical alterations are obvious. Additionally, the technique described in this paper may permit the processes of cortical morphogenesis to be studied in living cells (10). This application may provide dynamic confirmation of the temporal reconstructions of morphogenesis based upon 6xed material.…”

Section: Discussionmentioning

confidence: 91%

Identification of the Basal Bodies and Kinetodesmal Fibers in Living Cells of Paramecium tetraurelia Sonneborn, 1975 and Paramecium sonneborni Aufderheide, Daggett & Nerad, 1983¹

Aufderheide

1986

The Journal of Protozoology

View full text Add to dashboard Cite

Critical use of Nomarski DIC optics and a rotocompressor permits basal bodies and kinetodesmal fibers to be visualized in the cortices of living Paramecium tetraurelia and Paramecium sonneborni. The identification of these structures is confirmed by the correspondence of the images obtained by DIC optics of living cells and by brightfield optics of fixed cells stained by the Fernández-Galiano silver technique. Examination of cells carrying cortical inversions (portions of the cortex rotated 180 degrees) shows that inverted regions may be identified and distinguished from normal regions by the orientation of the kinetodesmal fibers of the kinetids (cortical units) within the kineties (ciliary rows). This demonstrates that both the asymmetry and the polarity of each cortical unit may be assessed in the living cell. This technique has useful applications in the study of morphogenesis and patterning in living cells and for the screening of mutations and variants conferring altered cortical phenotypes.

show abstract

Section: Discussionmentioning

confidence: 91%

Identification of the Basal Bodies and Kinetodesmal Fibers in Living Cells of Paramecium tetraurelia Sonneborn, 1975 and Paramecium sonneborni Aufderheide, Daggett & Nerad, 1983¹

Aufderheide

1986

The Journal of Protozoology

View full text Add to dashboard Cite

show abstract

“…Cells on the verge of fission (predividers ca. interfission age 0-90 (Hanson, 1974)) are easily identifiable under the dissection microscope because of a slightly flattened ventral side and their larger size. From this stage they take about 25 min to complete fission, and although the furrow site has been established, it is not distinctly seen until after another 5-10 min.…”

Section: Methodsmentioning

confidence: 99%

Longitudinal microtubules and their functions during asexual reproduction inParamecium tetraurelia

Sundararaman

Hanson

1976

Genet. Res.

Self Cite

View full text Add to dashboard Cite

Longitudinal microtubules appear in Paramecium tetraurelia when cells are ready to divide (ca. interfission age 0-9). They arise in the longitudinal cortical ridges between kineties and form, in each ridge, an incomplete circlet of 15-18 microtubules, disappearing 10-15 min after separation of the fission products (ca. interfission age 0-03-0-04). Vinblastine, colchicine, colcemid, mercaptoethanol, and cold treatments all result in loss of these microtubules, rounding up of the cells, and some suppression of cell division. The tubules are thought to play a role in cellular elongation, morphogenesis, and separation.

show abstract

“…pyrif ormis, especially those having proclivities towards parasitism (21) and whose nutrition comprises some oddities (60). This projected standardization might provide leads to improving media for Paramecium; work remains to overcome difficulties reported in applying the defined medium for P. aurelia (156) to grow single cells of P. aurelia (54). It is not clear why this defined medium falls short in satisfying P. bursaria ( 138).…”

Section: Are Tetrahymena and Other Hymenostomes Representative Ciliates?mentioning

confidence: 99%

Search for Clues to the Evolutionary Meaning of Ciliate Phylogeny*†

Hutner

Corliss

1976

The Journal of Protozoology

View full text Add to dashboard Cite

Progress in ciliatology and in allied fields may demystify ciliate phylogenetics. Concentration on hymenostomes (mainly Tetrahymena and Paramecium) may have obscured directional features of ciliate physiology in phylogenetic problems. Therefore, means are suggested for "domesticating" the presumptively primitive, predominantly marine, sand-dwelling gymnostomes having nondividing macronuclei. The prize quarry is the marine psammophile Stephanopogon whose homokaryotic condition may mark it as a living fossil. Eventual axenic cultivation of these "primitive" ciliates may be aided by use as food of easily grown photosynthetic prokaryotes, some isolated from the marine sulfuretum or adjacent aerobic muds and sands where "karyorelictid" ciliates flourish.

show abstract

Chapter 18 Methods in the Cellular and Molecular Biology of Paramecium

Cited by 11 publications

References 192 publications

Identification of the Basal Bodies and Kinetodesmal Fibers in Living Cells of Paramecium tetraurelia Sonneborn, 1975 and Paramecium sonneborni Aufderheide, Daggett & Nerad, 1983¹

Identification of the Basal Bodies and Kinetodesmal Fibers in Living Cells of Paramecium tetraurelia Sonneborn, 1975 and Paramecium sonneborni Aufderheide, Daggett & Nerad, 1983¹

Longitudinal microtubules and their functions during asexual reproduction inParamecium tetraurelia

Search for Clues to the Evolutionary Meaning of Ciliate Phylogeny*†

Contact Info

Product

Resources

About

Chapter 18 Methods in the Cellular and Molecular Biology of Paramecium

Cited by 11 publications

References 192 publications

Identification of the Basal Bodies and Kinetodesmal Fibers in Living Cells of Paramecium tetraurelia Sonneborn, 1975 and Paramecium sonneborni Aufderheide, Daggett & Nerad, 19831

Identification of the Basal Bodies and Kinetodesmal Fibers in Living Cells of Paramecium tetraurelia Sonneborn, 1975 and Paramecium sonneborni Aufderheide, Daggett & Nerad, 19831

Longitudinal microtubules and their functions during asexual reproduction inParamecium tetraurelia

Search for Clues to the Evolutionary Meaning of Ciliate Phylogeny*†

Contact Info

Product

Resources

About

Identification of the Basal Bodies and Kinetodesmal Fibers in Living Cells of Paramecium tetraurelia Sonneborn, 1975 and Paramecium sonneborni Aufderheide, Daggett & Nerad, 1983¹

Identification of the Basal Bodies and Kinetodesmal Fibers in Living Cells of Paramecium tetraurelia Sonneborn, 1975 and Paramecium sonneborni Aufderheide, Daggett & Nerad, 1983¹