Chapter 17 Zebrafish/Mouse Somatic Cell Hybrids for the Characterization of the Zebrafish Genome

Ekker, Marc; Ye, Fengchun; Joly, Lucille; Tellis, Patricia; Chevrette, Mario

doi:10.1016/s0091-679x(08)61907-x

Cited by 8 publications

(7 citation statements)

References 23 publications

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“…Similarly, all seven markers of LG 3 were present in the ZFB212 hybrid, while both LFFB3 and LFFB10 contained all eight markers (45 cM apart) of LG 18. Since fluorescence in situ hybridization (FISH) analysis using whole zebrafish painting probe rarely detected whole zebrafish chromosomes standing by themselves in hybrids (7,8), it is possible that complete or nearly complete zebrafish chromosomes could be integrated between mouse chromosomal elements.…”

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confidence: 99%

“…PCR analysis of the zebrafish/mouse hybrids shows that hybrids have incorporated more zebrafish elements than previously determined by the FISH analysis. In fact, some hybrids had zebrafish fragments coming from as many as 11 different linkage groups (4,8). Therefore, either some of the elements are small enough to escape detection by FISH or, more likely, segments from two different chromosomes have integrated together inside a mouse chromosome.…”

Section: Fig 1-continuedmentioning

confidence: 99%

“…It is thus possible to culture some of the hybrids and specifically select for one specific chromosome. The resulting subclone can be used as a source of DNA for this zebrafish chromosome, for example, by inter-DANA/mermaid PCR (8). Furthermore, with a selection scheme similar to that used to produce the hybrids, one is able to transfer the tagged chromosome (or chromosomal fragment) into another recipient cell and thus assess the function of the genes transcribed from this region.…”

Section: Fig 1-continuedmentioning

confidence: 99%

“…We have previously generated a collection of somatic cell hybrids by fusing zebrafish fibroblasts with a mouse melanoma cell line (7,8). This somatic hybrid panel was used to anchor one of the two meiotic maps of the zebrafish genome (19).…”

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confidence: 99%

“…The production of zebrafish/mouse somatic cell hybrids between zebrafish ZF4 or LFF embryonic fibro-blasts and mouse melanoma cells has been described (7,8). The zebrafish/mouse hybrids called ZFB and LFFB are derived from the ZF4 and LFF zebrafish parental cells, respectively.…”

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Characterization of a Zebrafish/Mouse Somatic Cell Hybrid Panel

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confidence: 99%

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Characterization of a Zebrafish/Mouse Somatic Cell Hybrid Panel

et al. 2000

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Zebrafish (Danio rerio) Genome and Genetics

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Dissecting Hematopoiesis and Disease Using the Zebrafish

Amatruda

Zon

1999

Developmental Biology

148

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The study of blood has often defined paradigms that are relevant to the biology of other vertebrate organ systems. As examples, stem cell physiology and the structure of the membrane cytoskeleton were first described in hematopoietic cells. Much of the reason for these successes resides in the ease with which blood cells can be isolated and manipulated in vitro. The cell biology of hematopoiesis can also be illuminated by the study of human disease states such as anemia, immunodeficiency, and leukemia. The sequential development of the blood system in vertebrates is characterized by ventral mesoderm induction, hematopoietic stem cell specification, and subsequent cell lineage differentiation. Some of the key regulatory steps in this process have been uncovered by studies in mouse, chicken, and Xenopus. More recently, the genetics of the zebrafish (Danio rerio) have been employed to define novel points of regulation of the hematopoietic program. In this review, we describe the advantages of the zebrafish system for the study of blood cell development and the initial success of the system in this pursuit. The striking similarity of zebrafish mutant phenotypes and human diseases emphasizes the utility of this model system for elucidating pathophysiologic mechanisms. New screens for lineage-specific mutations are beginning, and the availability of transgenics promises a better understanding of lineage-specific gene expression. The infrastructure of the zebrafish system is growing with an NIH-directed genome initiative, providing a detailed map of the zebrafish genome and an increasing number of candidate genes for the mutations. The zebrafish is poised to contribute greatly to our understanding of normal and disease-related hematopoiesis.

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Chapter 17 Zebrafish/Mouse Somatic Cell Hybrids for the Characterization of the Zebrafish Genome

Cited by 8 publications

References 23 publications

Characterization of a Zebrafish/Mouse Somatic Cell Hybrid Panel

Characterization of a Zebrafish/Mouse Somatic Cell Hybrid Panel

Zebrafish (Danio rerio) Genome and Genetics

Dissecting Hematopoiesis and Disease Using the Zebrafish

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