Chapter 14: Quality Control of Radiopharmaceuticals

Hung, Joseph C.

doi:10.21019/9781582122830.ch14

Cited by 1 publication

(1 citation statement)

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“…Neutrophils were purified from whole blood from SLE patients and sex and age matched HDs using Histopaque-1077 (PAN-Biotech, Aidenbach, Germany) based density gradient centrifugation at 1800 rpm for 30 min at RT without a break, which separate erythrocytes and granulocytes from mononuclear cells [ 48 ]. Then, neutrophils were obtained from the lowest layer and erythrocytes were eliminated by hypotonic lysis with cold water [ 49 , 50 ].…”

Section: Methodsmentioning

confidence: 99%

Low P-Selectin Glycoprotein Ligand-1 Expression in Neutrophils Associates with Disease Activity and Deregulated NET Formation in Systemic Lupus Erythematosus

Muñoz‐Callejas

González‐Sánchez

Silván

et al. 2023

IJMS

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Systemic Lupus Erythematosus (SLE) is an autoimmune disease characterized by the generation of anti-DNA autoantibodies due to exposure of immune cells to excessive amounts of extracellular DNA. Lack of P-selectin in mice induces the development of a lupus-like syndrome and patients with cutaneous lupus have reduced P-selectin expression in skin vessels. Using flow cytometry we analyzed in healthy donors and patients the expression of P-selectin Glycoprotein Ligand-1 (PSGL-1) in circulating neutrophils and the implication of PSGL-1/P-selectin interaction in neutrophil extracellular traps (NETs) generation. We found a statistical significance that neutrophils from active SLE patients have a reduced expression of PSGL-1 and low levels of PSGL-1 in neutrophils from SLE patients associated with the presence of anti-dsDNA antibodies, clinical lung involvement, Raynaud’s phenomenon, and positive lupus anticoagulant. PSGL-1 is present along the DNA in the NET. In healthy donors, neutrophil interaction with immobilized P-selectin triggers Syk activation, increases the NETs percentage and reduces the amount of DNA extruded in the NETs. In active SLE patients, neutrophil interaction with P-selectin does not activate Syk or reduce the amount of DNA extruded in the NETs, that might contribute to increase the extracellular level of DNA and hence, to disease pathogenesis.

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Section: Methodsmentioning

confidence: 99%