Chapter 13 Thin-layer chromatography

Grillini, Pamela M.

doi:10.1016/s0166-526x(06)47013-6

Cited by 2 publications

(2 citation statements)

References 13 publications

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“…The time from sample preparation through TLC to measurement of UV absorbance of antiretrovirals in FDC (eight samples per plate) took an average of 100 min, while with the HPLC method more than 160 min was spent on the same number of samples (20 min per sample). This implied that multiple samples can be run at a single operation per plate [26].…”

Section: Resultsmentioning

confidence: 99%

“…[25] reported that the demonstrated capability and efficiency of TLC in direct resolution of the racemate clearly establish its superiority over HPLC. TLC provides the most simple, rapid, and cost‐effective method with low volume consumption of solvents and many samples could be run simultaneously on a plate [17,26]. Also, in recent times TLC has been hyphenated to other detection devices or instruments, such as TLC–MS [22], UTLC–MALDI–MS [27], HPTLC–UV [22], and TLC–MALDI–TOF‐MS [28].…”

Section: Introductionmentioning

confidence: 99%

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Simple thin layer chromatography–ultraviolet spectrophotometric method for quality assessment of binary fixed‐dose‐combinations of lamivudine/tenofovir disoproxil fumarate and lamivudine/zidovudine in tablet formulations

Vaikosen

Kashimawo

Soyinka

et al. 2020

J of Separation Science

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Antiretroviral fixed-dose-combination drugs are best assayed with high-performance liquid chromatography, or liquid chromatography-tandem mass spectrometry. However, most scientists in developing nations have no access to these expensive instruments. A more affordable quantitative technique is the use of ultraviolet-visible spectroscopy-where often the absorption spectra of these antiretrovirals are overlapping; thus complex derivative methodologies are required for quantification. A simple, rapid, and accurate thin layer chromatography-ultraviolet spectrophotometric method for the quantification of binary mixtures of lamivudine, zidovudine, and tenofovirdisoproxil-fumarate in tablet formulations was developed. Lamivudine/tenofovirdisoproxil-fumarate and lamivudine/zidovudine were extracted and separated on glass thin-layer chromatography plates. Drugs were identified in ultraviolet light at 254 nm and quantified in acidic medium using ultraviolet spectrophotometry. The retardation factors were 0.43, 0.79, and 0.81 for lamivudine, tenofovir-disoproxilfumarate, and zidovudine, respectively, with corresponding absorption maxima at 270, 260, and 265 nm. Linearity ranged from 1 to 40 µg/mL for all drugs (R = 0.9998-0.9999), while recovery studies were 95.10-102.11% and amount in formulations ranged from 97.99 ± 0.63 to 101.47 ± 2.39%. The paired t-test (n = 5) indicated no significant difference between the proposed and high-performance liquid chromatography methods, hence comparable and can be used as an alternative method in routine quality determination of antiretroviral medicines. K E Y W O R D S

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Simple thin layer chromatography–ultraviolet spectrophotometric method for quality assessment of binary fixed‐dose‐combinations of lamivudine/tenofovir disoproxil fumarate and lamivudine/zidovudine in tablet formulations

Vaikosen

Kashimawo

Soyinka

et al. 2020

J of Separation Science

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Overview of High-Performance Thin Layer Chromatography and its Applications in Pesticide Analysis

Kandasamy

Muruganantham

Krishnaswami

et al. 2023

CAC

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In recent decades, increased demand for food has been caused by a rapid rise in the human population, which triggers agricultural intensification. To resist undesired pests from infecting crops, farmers widely utilize pesticides to improve agricultural production during the pre-harvest period. Despite the fact that pesticides cause a number of health risks, there is insufficient monitoring of these toxins. Therefore, it is important to develop a specific, accurate, and efficient method for determining the pesticides in varied samples in order to safeguard health against potential risks. Due to the lower concentrations of active compounds and their diversity of availability, it is challenging to detect pesticide residues in different samples. In this case, to effectively separate, identify, and accurately quantify pesticides at low concentrations in a variety of samples, a reliable analytical methodology is needed. Recently, the application of high-performance thin layer chromatography (HPTLC) offers a wider scope with excellent separation, identification, and quantitative/qualitative determination in pesticide analysis. In spite of their extremely low quantities, pesticide residues can be accurately and precisely identified using HPTLC. HPTLC has a number of benefits, such as easy sample preparation, automation, densitometry, and hyphenation, and is particularly well suited for identification and detection. Concerning this, the proposed review paper provides an overview of stationary phases, mobile phases, sample applicators, visualization, derivatization, and detection of HPTLC utilized for the identification and detection of pesticide residues in agriculture and environmental samples.

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Chapter 13 Thin-layer chromatography

Cited by 2 publications

References 13 publications

Simple thin layer chromatography–ultraviolet spectrophotometric method for quality assessment of binary fixed‐dose‐combinations of lamivudine/tenofovir disoproxil fumarate and lamivudine/zidovudine in tablet formulations

Simple thin layer chromatography–ultraviolet spectrophotometric method for quality assessment of binary fixed‐dose‐combinations of lamivudine/tenofovir disoproxil fumarate and lamivudine/zidovudine in tablet formulations

Overview of High-Performance Thin Layer Chromatography and its Applications in Pesticide Analysis

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