Chapter 12 Methods and Applications of Flow Systems for Analysis and Sorting of Mammalian Cells

A new method for the analysis of plasma cell DNA content in multiple myeloma samples using a CD38/propidium iodide double staining technique

“…In addition, there was no correlation between the S-phase of the neoplastic and normal bone marrow cells (r = 0.22; P > 0.10); this work therefore shows that the assessment of the total proliferative rate of bone marrow samples does not reflect either the proliferation of normal cells or that of neoplastic plasma cells but will depend on the proliferative rate and the percentage of each population within the sample, which can be assessed by the technique described here. Key terms: Flow cytometry, DNA ploidy, cell cycle, S-phase, multiple myeloma Quantitative measurements of tumor cell DNA content using flow cytometry (FCM) have been performed for over two decades (1,9,10,25,26,28,39). These studies have basically provided two different types of information on the biology of neoplastic cells: (1) the existence or absence of clonal abnormalities in cell DNA content, DNA aneuploidy, and (2) the analysis of the distribution of a certain cell population through the different cell cycle phases.…”

DNA Content Analysis by Flow Cytometry and Cytogenetic Analysis in Mycosis Fungoides and Sézary Syndrome

“…FCM can rapidly analyze DNA content of large numbers of cells and is not dependent on the proliferative state of a tumor (9)(10)(11). Because living cells are not essential for the analysis, the cytoplasm can be lysed, allowing for easy cell dispersion (10).…”

“…Cutaneous T-cell lymphomas, including mycosis fungoides and the S6zary syndrome, are malignant disorders of thymus-derived lymphocytes (T cells) (18)(19)(20) 11 at diagnosis and remission, 4 at diagnosis and relapse, 2 at diagnosis, remission, and relapse, 1 only in remission, and 7 only in relapse. Six of the eight patients studied only in remission or relapse had previously received systemic chemotherapy and one had previously received whole body electron beam irradiation.…”

Quantitative immunofluorescence in single Saccharomyces cerevisiae cells

“…The binding of PI to double-stranded DNA is loose and is hindered by fixation of cells with formaldehyde (6,7). Because the parameters of the fixation portion of the stain were determined to optimize immunofluorescent staining and prevention of cell loss, no changes were made to try to optimize cell fixation for the DNA stain as were made by Clevenger et al (6).…”