Chapter 10 Vital Staining of Plant Cells

Stadelmann, E.; Kinzel, Helmut

doi:10.1016/s0091-679x(08)60717-7

Cited by 46 publications

(43 citation statements)

References 39 publications

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“…8 than 6 . 2 (comparative data not shown), in line with the well-known fact that these dyes enter living cells mostly in nonionized form [24] . Nile blue gave the highest contrast between haustorium and host and also stained appressoria and hyphae intensely [Figs .…”

Section: Resultssupporting

confidence: 73%

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Accumulation of potentiometric and other dyes in haustoria of Erysiphe graminis in living host cell

Bushnell

Mendgen

Liu

1987

Physiological and Molecular Plant Pathology

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Section: Resultssupporting

confidence: 73%

“…Many vital dyes have been shown to enter living cells of higher plants and fungi, usually by passive diffusion [7,24] . These dyes often accumulate within cells by being bound to intracellular components or by becoming ionized at the pH levels prevailing within the vacuole and therefore unable to exit through the tonoplast .…”

Section: Introductionmentioning

confidence: 99%

Accumulation of potentiometric and other dyes in haustoria of Erysiphe graminis in living host cell

Bushnell

Mendgen

Liu

1987

Physiological and Molecular Plant Pathology

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“…(and hence are likely membrane bound), contain compounds with affinity for NR (such as other phenolics [Stadelmann and Kinzel, 1972]), or a combination of both.…”

Section: Anthocyanin-accumulating Subvacuolar Structures In Arabidopsismentioning

confidence: 99%

A Trafficking Pathway for Anthocyanins Overlaps with the Endoplasmic Reticulum-to-Vacuole Protein-Sorting Route in Arabidopsis and Contributes to the Formation of Vacuolar Inclusions

Poustka¹,

Irani²,

Feller³

et al. 2007

Plant Physiology

204

230

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Plants produce a very large number of specialized compounds that must be transported from their site of synthesis to the sites of storage or disposal. Anthocyanin accumulation has provided a powerful system to elucidate the molecular and cellular mechanisms associated with the intracellular trafficking of phytochemicals. Benefiting from the unique fluorescent properties of anthocyanins, we show here that in Arabidopsis (Arabidopsis thaliana), one route for anthocyanin transport to the vacuole involves vesicle-like structures shared with components of the secretory pathway. By colocalizing the red fluorescence of the anthocyanins with green fluorescent protein markers of the endomembrane system in Arabidopsis seedlings, we show that anthocyanins are also sequestered to the endoplasmic reticulum and to endoplasmic reticulum-derived vesicle-like structures targeted directly to the protein storage vacuole in a Golgi-independent manner. Moreover, our results indicate that vacuolar accumulation of anthocyanins does not depend solely on glutathione S-transferase activity or ATP-dependent transport mechanisms. Indeed, we observed a dramatic increase of anthocyanin-filled subvacuolar structures, without a significant effect on total anthocyanin levels, when we inhibited glutathione S-transferase activity, or the ATP-dependent transporters with vanadate, a general ATPase inhibitor. Taken together, these results provide evidence for an alternative novel mechanism of vesicular transport and vacuolar sequestration of anthocyanins in Arabidopsis.

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“…Neutral red is a dye that primarily accumulates in vacuoles and vesicles (Mahlberg, 1972;Stadelmann and Kinzel, 1972) but has also been shown to stain cell walls (Stadelmann and Kinzel, 1972). The color of neutral red staining can vary depending on the contents and pH of the vacuole or vesicle (Mahlberg, 1972;Stern et al, 1986).…”

Section: Dna and Vacuolementioning

confidence: 99%

Cytochemical Analysis of Pollen Development in Wild-Type Arabidopsis and a Male-Sterile Mutant.

1990

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Microsporogenesis has been examined in wild-type Arabidopsis thaliana and the nuclear male-sterile mutant BM3 by cytochemical staining. The mutant lacks adenine phosphoribosyltransferase, an enzyme of the purine salvage pathway that converts adenine to AMP. Pollen development in the mutant began to diverge from wild type just after meiosis, as the tetrads of microspores were released from their callose walls. The first indication of abnormal pollen development in the mutant was a darker staining of the microspore wall due to an incomplete synthesis of the intine. Vacuole formation was delayed and irregular in the mutant, and the majority of the mutant microspores failed to undergo mitotic divisions. Enzyme activities of alcohol dehydrogenase and esterases decreased in the mutant soon after meiosis and were undetectable in mature pollen grains of the mutant. RNA accumulation was also diminished. These results are discussed in relation to the possible role@) of adenine salvage in pollen development.

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Chapter 10 Vital Staining of Plant Cells

Cited by 46 publications

References 39 publications

Accumulation of potentiometric and other dyes in haustoria of Erysiphe graminis in living host cell

Accumulation of potentiometric and other dyes in haustoria of Erysiphe graminis in living host cell

A Trafficking Pathway for Anthocyanins Overlaps with the Endoplasmic Reticulum-to-Vacuole Protein-Sorting Route in Arabidopsis and Contributes to the Formation of Vacuolar Inclusions

Cytochemical Analysis of Pollen Development in Wild-Type Arabidopsis and a Male-Sterile Mutant.

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