Chapter 1 Culture and Microsurgical Manipulation of the Early Avian Embryo

Selleck, Mark A. J.

doi:10.1016/s0091-679x(08)60620-2

Cited by 36 publications

(23 citation statements)

References 36 publications

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“…Eggs are handled routinely as described in the literature (e.g., Tickle, 1993;Selleck, 1996;Saunders, 1996). Briefly, eggs are incubated at 95% humidity and 38.5°C to the required embryonic stage (see above).…”

Section: Egg Preparationmentioning

confidence: 99%

In ovo electroporation of avian somites

Scaal

Gros

Lesbros

et al. 2004

Developmental Dynamics

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Section: Egg Preparationmentioning

confidence: 99%

In ovo electroporation of avian somites

Scaal

Gros

Lesbros

et al. 2004

Developmental Dynamics

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“…The administration of AS was as follows: normal quail eggs were placed horizontally and incubated at 37°C, at 60% humidity for 20 -26 hr. Windowing of eggs and injections were performed by standard techniques (Selleck, 1996). Staging was according to Hamburger and Hamilton (1951).…”

Section: As Treatment To Block Receptor Actionmentioning

confidence: 99%

Function of RARγ and RARα2 at the initiation of retinoid signaling is essential for avian embryo survival and for distinct events in cardiac morphogenesis

Romeih

Cui

Michaille

et al. 2003

Developmental Dynamics

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“…For local CsA treatments, 1-mm agar squares (2% agar in PBS) were soaked in 10 mg/ml CsA for at least 1 hr and placed directly on E1 (stages 7-8) or E2 (stages 11-12) embryos. Eggs were windowed by using standard procedures, resealed with tape, and incubated 1-2 days further (Selleck, 1996). After treatment, morphology and development of the cardiovascular system was assessed visually and documented photographically.…”

Section: Cyclosporin Treatmentsmentioning

confidence: 99%

Calcineurin signaling in avian cardiovascular development

Liberatore

Yutzey

2003

Developmental Dynamics

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Experiments were initiated in avian embryos to determine the embryonic expression of calcineurin protein phosphatase isoforms as well as to identify developmental processes affected by inhibition of calcineurin signal transduction. Chicken calcineurin A alpha (CnA␣) and calcineurin A beta (CnA␤) are differentially expressed in the developing cardiovascular system, including primitive heart tube and valve primordia. Inhibition of calcineurin signaling by cyclosporin A (CsA) treatment in ovo resulted in distinct cardiovascular malformations, depending on the timing and localization of treatment. Initial formation of the heart tube was apparently normal in embryos treated with CsA from embryonic day (E)1 to E2, but hallmarks of heart failure were apparent with treatment from E2 to E3. Vascular defects were apparent in whole embryos treated on either day, but local administration of CsA directly to the forming vessels on E2 did not inhibit blood vessel formation. This observation supports an indirect effect of calcineurin inhibition on angiogenic remodeling as a result of compromised heart development. Together these studies are consistent with multiple roles for calcineurin signaling in the developing cardiovascular system.

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Chapter 1 Culture and Microsurgical Manipulation of the Early Avian Embryo

Cited by 36 publications

References 36 publications

In ovo electroporation of avian somites

In ovo electroporation of avian somites

Function of RARγ and RARα2 at the initiation of retinoid signaling is essential for avian embryo survival and for distinct events in cardiac morphogenesis

Calcineurin signaling in avian cardiovascular development

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