Chaperoning histones at the DNA repair dance

Chakraborty, Ujani; Shen, Zih-Jie; Tyler, Jessica K.

doi:10.1016/j.dnarep.2021.103240

Cited by 13 publications

(10 citation statements)

References 147 publications

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“…Indeed, the dense constitutive heterochromatin structure hinders an efficient DNA repair process and thus requires decompaction (Schieferstein and Thomä, 1998; Liu, 2015). Alteration of chromatin structure occurs during several DNA repair processes and often results from a change in nucleosome density (Waters et al ., 2015; Tripuraneni et al ., 2021; Chakraborty et al ., 2021). Few emerging models proposed that different chromatin remodeling events facilitate DNA repair, including nucleosome PTM erasure (Palomera-Sanchez et al ., 2010; Jeon et al ., 2020), nucleosome sliding and eviction (Dinant et al ., 2012; Matsumoto et al ., 2019; Nodelman and Bowman, 2021; Chakraborty et al ., 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Alteration of chromatin structure occurs during several DNA repair processes and often results from a change in nucleosome density (Waters et al ., 2015; Tripuraneni et al ., 2021; Chakraborty et al ., 2021). Few emerging models proposed that different chromatin remodeling events facilitate DNA repair, including nucleosome PTM erasure (Palomera-Sanchez et al ., 2010; Jeon et al ., 2020), nucleosome sliding and eviction (Dinant et al ., 2012; Matsumoto et al ., 2019; Nodelman and Bowman, 2021; Chakraborty et al ., 2021). Importantly, our results show that constitutive heterochromatin dynamics predominantly depends on the modulation of H3K9me2 content and not of H3K27me1.…”

Section: Discussionmentioning

confidence: 99%

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The histone demethylase JMJ27 acts during the UV-induced modulation of H3K9me2 landscape and facilitates photodamage repair

Johann to Berens,

Peter,

Koechler

et al. 2024

Preprint

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Plants have evolved sophisticated DNA repair mechanisms to cope with the deleterious effects of UV-induced DNA damage. Indeed, DNA repair pathways cooperate with epigenetic-related processes to efficiently maintain genome integrity. However, it remains to be deciphered how photodamages are recognized within different chromatin landscapes, especially in compacted genomic regions such as constitutive heterochromatin. We combined cytogenetics and epigenomics to identify that UV-C irradiation induces modulation of the main epigenetic mark found in constitutive heterochromatin, H3K9me2. We demonstrated that the histone demethylase, Jumonji27 (JMJ27), is responsible for the UV-induced reduction of H3K9me2 content at chromocenters. In addition, we identified that JMJ27 forms a complex with the photodamage recognition factor, DNA Damage Binding protein 2 (DDB2), and that the fine tuning of H3K9me2 contents orchestrates DDB2 dynamics on chromatin in response to UV-C exposure. Hence, this study uncovers the existence of an interplay between photodamage repair and H3K9me2 homeostasis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The histone demethylase JMJ27 acts during the UV-induced modulation of H3K9me2 landscape and facilitates photodamage repair

Johann to Berens,

Peter,

Koechler

et al. 2024

Preprint

View full text Add to dashboard Cite

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“…Lastly, there is MacroH2A, an H2A variant which, besides being enriched on the inactive X chromosome ( Costanzi and Pehrson, 1998 ) and facultative heterochromatin ( Gamble et al, 2010 ), has been associated with different DDR pathways, as Caron et al (2021) nicely cover in their review. For more detailed revisions on histone variants and chaperones involved in DNA damage and repair, please refer to Ferrand et al (2020) , Caron et al (2021) , and Chakraborty et al (2021) .…”

Section: The Triad Of Nucleosomes Disruptionmentioning

confidence: 99%

Histones and their chaperones: Adaptive remodelers of an ever-changing chromatinic landscape

et al. 2022

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Chromatin maintenance and remodeling are processes that take place alongside DNA repair, replication, or transcription to ensure the survival and adaptability of a cell. The environment and the needs of the cell dictate how chromatin is remodeled; particularly where and which histones are deposited, thus changing the canonical histone array to regulate chromatin structure and gene expression. Chromatin is highly dynamic, and histone variants and their chaperones play a crucial role in maintaining the epigenetic regulation at different genomic regions. Despite the large number of histone variants reported to date, studies on their roles in physiological processes and pathologies are emerging but continue to be scarce. Here, we present recent advances in the research on histone variants and their chaperones, with a focus on their importance in molecular mechanisms such as replication, transcription, and DNA damage repair. Additionally, we discuss the emerging role they have in transposable element regulation, aging, and chromatin remodeling syndromes. Finally, we describe currently used methods and their limitations in the study of these proteins and highlight the importance of improving the experimental approaches to further understand this epigenetic machinery.

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“…The connection between chromatin and these DNA repair pathways is intricate in eukaryotes, with many factors in uencing chromatin dynamics [8]. These factors encompass histone post-translational modi cations (PTMs), histone variants [9], chaperones [10], chromatin remodeling complexes [11], and more. Recent studies have identi ed various types of modi cations (PTMs), including methylation, acetylation, phosphorylation, ADP ribosylation, ubiquitylation, and SUMOylation, and their relationship with diseases has been explored [12], [13].…”

Section: Introduction 11 the Signi Cance Of Genetic Materialsmentioning

confidence: 99%

The Impact of Histone Post-translational Modifications H3K9me on Same Gene Mutation Rate in Fission Yeast

Abdalla,

Walker

2024

Preprint

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Mutations are the driving force behind genetic variation, fueling both the oncogenesis and evolution of species. The mutation rate varies across the genome, potentially in response to chromatin organization by histone modifications and other factors. However, the exact relationship between the two is yet to be fully understood and requires further investigation. One modification involves the methylation of histone H3 at lysine 9, which creates heterochromatin and represses transcription in euchromatin to maintain genome stability for organism survival. This study aimed to determine the effect of H3K9 methylation alone, without other histone markers, on the mutation rate in fission yeast using fluctuation assays and statistical analysis. Our groundbreaking method has been proven to accurately estimate mutation rates of a single gene under two different conditions in a single experiment using one isogenic clone. Our research results demonstrate that the H3K9me markers increase the phenotypic mutation rate of the same gene. For prospective researchers, this study presents an innovative experimental approach that ensures unparalleled accuracy in gene analysis for genetics applications and epigenetic therapy.

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Chaperoning histones at the DNA repair dance

Cited by 13 publications

References 147 publications

The histone demethylase JMJ27 acts during the UV-induced modulation of H3K9me2 landscape and facilitates photodamage repair

The histone demethylase JMJ27 acts during the UV-induced modulation of H3K9me2 landscape and facilitates photodamage repair

Histones and their chaperones: Adaptive remodelers of an ever-changing chromatinic landscape

The Impact of Histone Post-translational Modifications H3K9me on Same Gene Mutation Rate in Fission Yeast

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