Chaperone Proteins in the Central Nervous System and Peripheral Nervous System after Nerve Injury

Ousman, Shalina S.; Frederick, Ariana; Lim, Erin-Mai F.

doi:10.3389/fnins.2017.00079

Cited by 19 publications

(10 citation statements)

References 93 publications

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“…These proteins express abundantly in the glial and neuronal cells and involved in regulating the inflammatory responses, cell homeostasis and cell survival. In addition the heat shock proteins show altered expression in the central and peripheral nervous system to support the axonal regeneration and remyelination upon nerve injuries like cerebral ischemia [80] . The transcriptome dataset of earthworm E. fetida denoted the presence of total 65 heat shock protein transcripts.…”

Section: Resultsmentioning

confidence: 99%

Annotation of nerve cord transcriptome in earthworm Eisenia fetida

et al. 2017

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In annelid worms, the nerve cord serves as a crucial organ to control the sensory and behavioral physiology. The inadequate genome resource of earthworms has prioritized the comprehensive analysis of their transcriptome dataset to monitor the genes express in the nerve cord and predict their role in the neurotransmission and sensory perception of the species. The present study focuses on identifying the potential transcripts and predicting their functional features by annotating the transcriptome dataset of nerve cord tissues prepared by Gong et al., 2010 from the earthworm Eisenia fetida. Totally 9762 transcripts were successfully annotated against the NCBI nr database using the BLASTX algorithm and among them 7680 transcripts were assigned to a total of 44,354 GO terms. The conserve domain analysis indicated the over representation of P-loop NTPase domain and calcium binding EF-hand domain. The COG functional annotation classified 5860 transcript sequences into 25 functional categories. Further, 4502 contig sequences were found to map with 124 KEGG pathways. The annotated contig dataset exhibited 22 crucial neuropeptides having considerable matches to the marine annelid Platynereis dumerilii, suggesting their possible role in neurotransmission and neuromodulation. In addition, 108 human stem cell marker homologs were identified including the crucial epigenetic regulators, transcriptional repressors and cell cycle regulators, which may contribute to the neuronal and segmental regeneration. The complete functional annotation of this nerve cord transcriptome can be further utilized to interpret genetic and molecular mechanisms associated with neuronal development, nervous system regeneration and nerve cord function.

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Section: Resultsmentioning

confidence: 99%

Annotation of nerve cord transcriptome in earthworm Eisenia fetida

et al. 2017

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“…Several studies have noted that the heat shock protein 90 family facilitates cell signaling; assists in the efficient folding of newly translated proteins intracellular transport, maintenance and degradation of proteins; and the protection of mesenchymal stem cells from apoptosis and stimulates their migration. Furthermore, their roles in calcium homeostasis, neuron survival, axonal regeneration, and neuroprotection of the central and peripheral nervous systems have been shown (Loones et al, 2000; Gao et al, 2015; Ousman et al, 2017). The pharmacological inhibition of HSP 90 has been associated with neurodegenerative disease, suggesting the protective role of these proteins (Luo et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Target Proteins in the Dorsal Hippocampal Formation Sustain the Memory-Enhancing and Neuroprotective Effects of Ginkgo biloba

et al. 2019

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We have previously shown that standardized extracts of Ginkgo biloba (EGb) modulate fear memory formation, which is associated with CREB-1 (mRNA and protein) upregulation in the dorsal hippocampal formation (dHF), in a dose-dependent manner. Here, we employed proteomic analysis to investigate EGb effects on different protein expression patterns in the dHF, which might be involved in the regulation of CREB activity and the synaptic plasticity required for long-term memory (LTM) formation. Adult male Wistar rats were randomly assigned to four groups (n = 6/group) and were submitted to conditioned lick suppression 30 min after vehicle (12% Tween 80) or EGb (0.25, 0.50, and 1.00 g⋅kg−1) administration (p.o). All rats underwent a retention test session 48 h after conditioning. Twenty-four hours after the test session, the rats were euthanized via decapitation, and dHF samples were removed for proteome analysis using two-dimensional polyacrylamide gel electrophoresis, followed by peptide mass fingerprinting. In agreement with our previous data, no differences in the suppression ratios (SRs) were identified among the groups during first trial of CS (conditioned stimulus) presentation (P > 0.05). Acute treatment with 0.25 g⋅kg−1 EGb significantly resulted in retention of original memory, without prevent acquisition of extinction within-session. In addition, our results showed, for the first time, that 32 proteins were affected in the dHF following treatment with 0.25, 0.50, and 1.00 g⋅kg−1 doses of EGb, which upregulated seven, 19, and five proteins, respectively. Additionally, EGb downregulated two proteins at each dose. These proteins are correlated with remodeling of the cytoskeleton; the stability, size, and shape of dendritic spines; myelin sheath formation; and composition proteins of structures found in the membrane of the somatodendritic and axonal compartments. Our findings suggested that EGb modulates conditioned suppression LTM through differential protein expression profiles, which may be a target for cognitive enhancers and for the prevention or treatment of neurocognitive impairments.

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“…The intermittent fasting regimen enhanced endogenous protective mechanisms, such as the chaperone and autophagy-lysosomal pathways, and prevented the inflammatory and degenerative changes associated with neuropathy. This resulted in improved locomotor performance and increased grip strength in the mice [46,47,48,49].…”

Section: Introductionmentioning

confidence: 99%

Charcot-Marie-Tooth: From Molecules to Therapy

Morena

Gupta

Hoyle

2019

IJMS

127

105

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Charcot-Marie-Tooth (CMT) is the most prevalent category of inherited neuropathy. The most common inheritance pattern is autosomal dominant, though there also are X-linked and autosomal recessive subtypes. In addition to a variety of inheritance patterns, there are a myriad of genes associated with CMT, reflecting the heterogeneity of this disorder. Next generation sequencing (NGS) has expanded and simplified the diagnostic yield of genes/molecules underlying and/or associated with CMT, which is of paramount importance in providing a substrate for current and future targeted disease-modifying treatment options. Considerable research attention for disease-modifying therapy has been geared towards the most commonly encountered genetic mutations (PMP22, GJB1, MPZ, and MFN2). In this review, we highlight the clinical background, molecular understanding, and therapeutic investigations of these CMT subtypes, while also discussing therapeutic research pertinent to the remaining less common CMT subtypes.

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Chaperone Proteins in the Central Nervous System and Peripheral Nervous System after Nerve Injury

Cited by 19 publications

References 93 publications

Annotation of nerve cord transcriptome in earthworm Eisenia fetida

Annotation of nerve cord transcriptome in earthworm Eisenia fetida

Target Proteins in the Dorsal Hippocampal Formation Sustain the Memory-Enhancing and Neuroprotective Effects of Ginkgo biloba

Charcot-Marie-Tooth: From Molecules to Therapy

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