Chaotropic salts impact in HPLC approaches for simultaneous analysis of hydrophilic and lipophilic drugs

Abstract

Simultaneous determination of drugs with different physicochemical properties necessitates thorough research and careful selection of high‐performance liquid chromatography conditions. In the present study, various concepts of high‐performance liquid chromatography method development for this aim have been discussed. Moreover, the work was motivated by the advantages of utilizing different chaotropic anions as a new promising approach to overcome the limitations of ion‐pairing agents commonly used for this purpose. Based on log P values, atorvastatin (log P = 6.36) and lisinopril (log P = –1.22) were chosen as representative examples for lipophilic and hydrophilic drugs, respectively. Several simple, economic, fast, and reliable high‐performance liquid chromatography methods were developed for their simultaneous analysis and are presented in a comparative manner, highlighting their advantages and limitations. Peak elution profile showed satisfying retentions and resolution about 3. Photo‐diode array calculations were exploited for identifying the molecules by their ultra‐violet spectra and peak purity, calculated and presented as rectangular‐shaped ratio grams. The linearity check showed excellent results and satisfactory system suitability parameters of both peaks. This confirms the investigation results and conclusions for the influence of the chaotropic salts on N‐containing molecules, by increasing their retentivities, and improving peak shapes, even on different quality columns without end‐capping and base‐deactivating of separation matrixes.

Figure 13 from: Peleshok K, Bondar B, Kryskiw L, Kucher T, Poliak O, Logoyda L (2021) Development and Validation of Spectrophotometric Methods for Estimation of Valsartan in Tablet Dosage Form. Pharmacia 68(4): 852-858. https://doi.org/10.3897/pharmacia.68.e73559

“…An important aspect of modern pharmaceutical analysis is compliance with the principles of green chemistry, which must be taken into account when developing analytical methods for the determination of valsartan in substances and drugs (Van Aken K. et al 2006;Peleshok K. et al 2021aPeleshok K. et al , 2021bGałuszka A. et al 2012;Shulyak N. et al 2021;Yuryeva O. et al 2018;Polyauk O. and Logoyda L. 2017;Mykhalkiv M. et al 2018;Logoyda L. et al 2018). Table 5 summarizes the results of developed methods found to be an excellent green analysis with a score of 89 for both.…”

Figure 9 from: Peleshok K, Bondar B, Kryskiw L, Kucher T, Poliak O, Logoyda L (2021) Development and Validation of Spectrophotometric Methods for Estimation of Valsartan in Tablet Dosage Form. Pharmacia 68(4): 852-858. https://doi.org/10.3897/pharmacia.68.e73559

“…An important aspect of modern pharmaceutical analysis is compliance with the principles of green chemistry, which must be taken into account when developing analytical methods for the determination of valsartan in substances and drugs (Van Aken K. et al 2006;K. et al 2021a, 2021bGałuszka A. et al 2012;Shulyak N. et al 2021;Yuryeva O. et al 2018;Polyauk O. and Logoyda L. 2017;Mykhalkiv M. et al 2018;Logoyda L. et al 2018). Table 5 summarizes the results of developed methods found to be an excellent green analysis with a score of 89 for both.…”

Study of the dissolution kinetics of drugs in solid dosage form with lisinopril and atorvastatin and intestinal permeability to assess their equivalence in vitro

“…Atorvastatin and lisinopril are a successful combination for the treatment of patients with chronic heart failure and hypertension (Naveed 2015;GoodRx undated). Analytical methods of analysis (Leis et al 1998(Leis et al , 1999El Gindy et al 2001;Andreas et al 2003;El-Emam et al 2004;Beasley et al 2005;Rahman et al 2005aRahman et al , 2005bHuang et al 2006;Stanisz and Kania 2006;Chaudhari et al 2007;Ivanovic et al 2007;Shah et al 2007;Basavaiah et al 2009;Chauhan et al 2011;Jamakhandi et al 2011;Sultana et al 2011;Kumar et al 2012;Naveed et al 2012;Arayne et al 2013;Hafez et al 2014;Lakshmi and Sreedevi 2014;Sathiyasundar and Valliappan 2014;Sbârcea et al 2014;Shulyak et al 2021aShulyak et al , 2021bShulyak et al , 2021c have been developed for the determination of atorvastatin and lisinopril in medicines and biological liquids. However, many of these methods are limited in their usage.…”