Channel Catfish Follicle-Stimulating Hormone and Luteinizing Hormone: Complementary DNA Cloning and Expression During Ovulation

Liu, Zhanjiang; Kim, Soonhag; Karsi, Attila

doi:10.1007/s1012601-0067-5

Cited by 34 publications

(12 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Since duality of GtHs has been reported widely from primitive teleosts (Kajimura et al 2001b;Kato et al 1993;Liu et al 2001;Suzuki et al 1988a;Swanson et al 1991;Van Der Kraak et al 1992;Yoshida et al 2001;Yoshiura et al 1997Yoshiura et al ,1999 to advanced teleosts (Copeland and Thomas 1993;Elizur et al 1996;García-Hernández et al 1997;Hassin et al 1995;Jackson et al 1999;Kajimura et al 2001a;Koide et al 1993;Lin et al 1992;Mateos et al 2003;Okada et al 1994;Tanaka et al 1993), the absence of antiFh FSHβ 50-60 positive cells is probably caused by lack of immunoreactivity from lower similarities in the antigen region (the amino acid sequences of the region have 36-55% similarity between the mummichog and primitive teleosts of Elopomorpha, Ostariophysi, and Protacanthopterygii; Table 4). …”

Section: Discussionmentioning

confidence: 99%

Universal antisera for immunocytochemical identification of two different gonadotrophs in acanthopterygian fishes

Shimizu

Sakai

Nashida

et al. 2003

Fish Physiol Biochem

View full text Add to dashboard Cite

Pituitaries of various teleosts belonging to 25 orders were immunostained with antisera raised against synthetic fragment peptides corresponding to conservative regions of gonadotropinβ subunits (mummichog FSHβ 50-60 and mummichog LHβ 91-106). Both immunoreactive FSH cells and immunoreactive LH cells were successfully identified in the fishes of almost every order of the superorder Acanthopterygii and several species of the superorders Paracanthopterygii and Polymixiomorpha, such as mullet, alfonsino, flyingfish, mackerel, flounder, cod, beardfish, etc. These antisera are therefore considered as universal antisera for immunocytochemical application to acanthopterygian fishes. Extensive diversity in the abundance of the FSH cells and the LH cells among species was noted even in fishes with similar gonadal stages, indicating the possibility that the respective roles of FSH and LH may vary considerably among species in advanced teleosts.Evident but generally weak immunoreactivities to anti-mummichog LHβ 91-106 were observed in the fishes of the superorder Cyclosquamata; and slight or weak immunoreactivities to the antiserum were observed in the fishes of several more primitive taxa (superorder Stenopterygii, Protacanthopterygii, Ostariophysi, subdivision Clupeomorpha, and subdivision Elopomorpha). No immunoreactivity to anti-mummichog FSHβ 50-60 was observed in these fishes. These results are consistent with the phylogenetic status of the fishes and the degree of conservativeness in the amino acid sequences of the antigen regions.

show abstract

Section: Discussionmentioning

confidence: 99%

Universal antisera for immunocytochemical identification of two different gonadotrophs in acanthopterygian fishes

Shimizu

Sakai

Nashida

et al. 2003

Fish Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…Using such an approach, we have characterized a complete set of all 32 small ribosomal protein cDNAs [7] and a complete set of all 47 large ribosomal protein cDNAs [30] from channel catfish. Other genes we have analysed include: the myostatin gene [10]; gonadotropin (GnRH) α-subunit [17]; GnRH β-subunit 1 and GnRH β-subunit 2 [15]; the α-actin gene [8]; the creatine kinase gene [16]; a number of cytokine genes; a number of complement genes; and a large number of cytochrome P450 genes (Liu, unpublished).…”

Section: Systematic Characterization Of Genes And/or Full Length Cdnasmentioning

confidence: 99%

A review of catfish genomics: progress and perspectives

Liu

2003

Comp Funct Genom

Self Cite

View full text Add to dashboard Cite

Catfish is one of the lower teleosts whose genome research is important for evolutionary genomics. As the major aquaculture species in the USA, its genome research also has practical and economical implications. Much progress has been made in recent years, including the development of large numbers of molecular markers, the construction of framework genetic linkage maps, the identification of putative markers involved in performance traits, and the development of genomic resources. Repetitive elements have been identified and characterized in the catfish genome that should facilitate physical analysis of the catfish genome. A large number of genes or full-length cDNAs have been analysed using genomic approaches, providing information on gene structure, gene evolution and gene expression in relation to functions. Catfish genome research has come to a stage when physical mapping through BAC contig construction is greatly demanded, in order to develop regional markers for QTL analysis and for large-scale comparative mapping. The current effort in large-scale EST analysis and type I marker mapping should further enhance research efficiency through comparative mapping. Candidate gene identification is being accelerated through the use of cDNA microarrays.

show abstract

“…As shown in Figs 1 and 2, the E. japonicus LH β subunit has typical structural characteristics of fish LH β subunit such as 12 cysteines, one N‐linked glycosylation site and the conserved short amino acid sequences, which are important for heterodimerization, stability and metabolic fate of GTHs (Ulloa‐Aguirre et al , 1999; Liu et al , 2001). Power et al (1997) reported that C. pallasii LH β also has these characteristics.…”

Section: Discussionmentioning

confidence: 99%

“…As in terrestrial vertebrates, two types of GTH (GTH‐I and GTH‐II) have been identified in many representative groups of the teleost lineage such as Salmonidae (Itoh et al , 1988; Suzuki et al , 1988 a ; Sekine et al , 1989; Kato et al , 1993), Anguilliformes (Yoshiura et al , 1999; Kajimura et al , 2001 a ; Han et al , 2003), Cypriniformes (Chang et al , 1988, 1990; Yoshiura et al , 1997; So et al , 2005), Siluriformes (Rebers et al , 1997; Liu et al , 2001; Vischer et al , 2003), Perciformes (Copeland & Thomas, 1993; Hassin et al , 1995; Elizur et al , 1996; Rosenfeld et al , 1997; Jackson et al , 1999; Mateos et al , 2003), Pleuronectiformes (Kajimura et al , 2001 b ; Cerdà et al , 2008), Gasterosteiformes (Hellqvist et al , 2004) and Cyprinodontiformes (Lin et al , 1992; Shimizu & Yamashita, 2002). To date, the comparative and phylogenetic studies on fish GTHs have provided lines of evidence that fish GTH‐I and GTH‐II are homologues of vertebrate FSH and LH, respectively (Querat et al , 2000).…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and localization of the luteinizing hormone β subunit and glycoprotein hormone α subunit from Japanese anchovy Engraulis japonicus

Ohkubo

Katayama

Shimizu

2010

Journal of Fish Biology

View full text Add to dashboard Cite

Although Clupeiformes contain many economically important species, there is limited information on their reproductive physiology. To obtain more insight into reproductive mechanisms in clupeiform fishes, molecular cloning of the Japanese anchovy Engraulis japonicus luteinizing hormone beta (LHbeta) and glycoprotein hormone alpha (GPHalpha) subunits, and immunocytochemistry of gonadotrophs in the pituitary using antisera raised against the synthetic peptides for both subunits were carried out. The cDNAs for LHbeta and GPHalpha subunits consisted of 963 and 535 nucleotides encoding 141 and 122 amino acids, respectively. The deduced amino acid sequences of the E. japonicus LHbeta subunit showed a 60% similarity to the Pacific herring Clupea pallasii LHbeta subunit and 24-31% similarities to FSHbeta subunits of other fish species. The E. japonicus GPHalpha subunit showed 52-57% similarities to anguilliform and cypriniform GPHalpha subunits. Both the subunits have typical structural characteristics of each subunit such as N-linked glycosylation sites, conserved cysteine residues and highly conserved short amino acid sequences. These results indicate that cDNAs cloned in this study encode the E. japonicus LHbeta and GPHalpha subunits. Reverse-transcription polymerase chain reaction (RT-PCR) revealed that both the LHbeta and GPHalpha subunit genes were abundantly expressed in the pituitary, and the GPHalpha subunit was observed to be weakly expressed in the extrapituitary tissues. Immunocytochemistry of the E. japonicus pituitary showed that cells that immunoreacted with antiserum against the LHbeta subunit were distributed in the peripheral regions of proximal pars distalis, and these cells were also immunoreactive to antiserum against the GPHalpha subunit. An abundant number of both LHbeta and GPHalpha cells in the pituitary of matured fish were observed, in comparison with immature fish. These results indicate that the E. japonicus LH is involved in the final reproductive maturation as well as those of other teleosts.

show abstract

Channel Catfish Follicle-Stimulating Hormone and Luteinizing Hormone: Complementary DNA Cloning and Expression During Ovulation

Cited by 34 publications

References 41 publications

Universal antisera for immunocytochemical identification of two different gonadotrophs in acanthopterygian fishes

Universal antisera for immunocytochemical identification of two different gonadotrophs in acanthopterygian fishes

A review of catfish genomics: progress and perspectives

Molecular cloning and localization of the luteinizing hormone β subunit and glycoprotein hormone α subunit from Japanese anchovy Engraulis japonicus

Contact Info

Product

Resources

About