Changing of RGS Transcripts Levels by Low-Dose-Rate Ionizing Radiation in Mouse Testis

Abstract

Deleterious effects of high dose radiation exposure with high-dose-rate are unarguable, but they are still controversial in low-dose-rate. The regulator of G-protein signaling (RGS) is a negative regulator of G proteincoupled receptor (GPCR) signaling. In addition, it is reported that irradiation stress led to GPCR-mediated mitogen-activated protein kinase (MAPK) and phosphotidylinositol 3-kinase (PI3-k) signaling. The RGS mRNA expression profiles by whole body radiation with low-dose-rate has not yet been explored. In the present study, we, therefore, examined which RGS was modulated by the whole body radiation with low-dose-rate (3.49 mGy·h -1 ). Among 16 RGS expression tested, RGS6, RGS13 and RGS16 mRNA were down-regulated by low-dose-rate irradiation. This is the first report that whole body radiation with low-dose-rate can modulate the different RGS expression levels. These results are expected to reveal the potential target and/or the biomarker proteins associated with male testis toxicity induced by low-dose-rate irradiation, which might contribute to understanding the mechanism beyond the testis toxicity.

Regulatory Potential of Long Non-Coding RNAs (lncRNAs) in Boar Spermatozoa with Good and Poor Freezability

“…However, in another study, it was reported that membrane-bound receptors for hormones, cytokines, growth factors or neurotransmitters are implicated in various sperm functions, such as motility and capacitation [ 72 ]. Moreover, RGS18 belongs to the G-protein signaling family, which inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits [ 73 ] and it is expressed in the testis [ 74 ]. Our findings suggest that the co-expression of cis -regulating DElncRNA with GHR , THRB or RGS18 was associated with increased sperm cryo-damage, however, the functions of these genes in sperm freezability are unclear.…”