Changing Gly311 to an acidic amino acid in the MATE family protein DTX6 enhances Arabidopsis resistance to the dihydropyridine herbicides

Lv, Zeyu; Zhao, Mingming; Wang, Wenjing; Wang, Qi; Huang, Meng; Li, Chaoqun; Lian, Qichao; Xia, Jianjun; Qi, Ji; Xiang, Cheng‐Bin; Tang, Huiru; Ge, Xiaochun

doi:10.1016/j.molp.2021.09.002

Cited by 23 publications

(18 citation statements)

References 54 publications

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“…Although phenomena of herbicide resistance have been discovered for decades, and 26 molecular target sites of herbicides have been classified by the Resistance Action Committee ( , accessed on 1 January 2022), new molecular targets and variations involving herbicide resistance are consistently and continuously being discovered [ 38 , 39 , 40 , 41 , 42 , 43 ]. In this study, eight genes located in the mapped interval were functionally annotated, among which Zm00001eb361930 and gene.8455157, possibly encoding a class III aminotransferase and a cationic amino acid transporter, respectively, were detected for expression in the leaves during glufosinate treatment ( Figure 4 ; Table 2 ).…”

Section: Discussionmentioning

confidence: 99%

Genetic Analysis and Fine Mapping of ZmGHT1 Conferring Glufosinate Herbicide Tolerance in Maize (Zea mays L.)

Bao

Gao

et al. 2022

IJMS

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Weed interference in the crop field is one of the major biotic stresses causing dramatic crop yield losses, and the development of herbicide-resistant crops is critical for weed control in the application of herbicide technologies. To identify herbicide-resistant germplasms, we screened 854 maize inbreed lines and 25,620 seedlings by spraying them with 1 g/L glufosinate. One plant (L336R), possibly derived from a natural variation of line L336, was identified to have the potential for glufosinate tolerance. Genetic analysis validated that the glufosinate tolerance of L336R is conferred by a single locus, which was tentatively designated as ZmGHT1. By constructing a bi-parental population derived from L336R, and a glufosinate sensitive line L312, ZmGHT1 was mapped between molecular markers M9 and M10. Interestingly, genomic comparation between the two sequenced reference genomes showed that large scale structural variations (SVs) occurred within the mapped region, resulting in 2.16 Mb in the inbreed line B73, and 11.5 kb in CML277, respectively. During the fine mapping process, we did not detect any additional recombinant, even by using more than 9500 F2 and F3 plants, suspecting that SVs should also have occurred between L336R and L312 in this region, which inhibited recombination. By evaluating the expression of the genes within the mapped interval and using functional annotation, we predict that the gene Zm00001eb361930, encoding an aminotransferase, is the most likely causative gene. After glufosinate treatment, lower levels of ammonia content and a higher activity of glutamine synthetase (GS) in L336R were detected compared with those of L336 and L312, suggesting that the target gene may participate in ammonia elimination involving GS activity. Collectively, our study can provide a material resource for maize herbicide resistant breeding, with the potential to reveal a new mechanism for herbicide resistance.

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Section: Discussionmentioning

confidence: 99%

Genetic Analysis and Fine Mapping of ZmGHT1 Conferring Glufosinate Herbicide Tolerance in Maize (Zea mays L.)

Bao

Gao

et al. 2022

IJMS

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“…Enhanced activities of the relevant exporters would increase PQ resistance. The recently identified MATE efflux transporter DTX6 demonstrated this molecular mechanism ( Lv et al., 2021 ; Xia et al., 2021 ). The overexpression of DTX6D in Arabidopsis confers strong resistance to a near-commercial PQ dose ( Xia et al., 2021 ).…”

Section: Potential Strategies For Developing Pq-resistant Cropsmentioning

confidence: 95%

“…DTX6 is indeed involved in the cellular efflux of PQ, as demonstrated by mesophyll protoplasts of DTX6 -overexpressing plants that export approximately 1.25 times more PQ than those of the wild type, further confirming the efflux nature of this transporter. Similar findings for DTX6 were obtained independently by another research group and published back to back ( Lv et al., 2021 ). Lv et al.…”

Section: Arabidopsis Mutants With Enhanced Pq Resistance Con...mentioning

confidence: 99%

Understanding paraquat resistance mechanisms in Arabidopsis thaliana to facilitate the development of paraquat-resistant crops

Nazish

Huang

Zhang

et al. 2022

Plant Communications

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“…The AA residues in EPs can serve as critical sites for the binding of substrates, and the switching of AA residues is likely to alter the substrate affinity [ 78 ]. Besides, changes in salient residues of AA proved to correlate with EP-mediated drug resistance [ 79 ]. Some well-characterized MDR EPs membership of the RND family comprising MexAB-OprM, MtrCDE, AcrAB-TolC, CmeABC are important for bacterial survival and pathogenicity.…”

Section: Efflux Pump-mediated Multi-drug Resistance In Bacteriamentioning

confidence: 99%

What Approaches to Thwart Bacterial Efflux Pumps-Mediated Resistance?

et al. 2022

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An effective response that combines prevention and treatment is still the most anticipated solution to the increasing incidence of antimicrobial resistance (AMR). As the phenomenon continues to evolve, AMR is driving an escalation of hard-to-treat infections and mortality rates. Over the years, bacteria have devised a variety of survival tactics to outwit the antibiotic's effects, yet given their great adaptability, unexpected mechanisms are still to be discovered. Over-expression of efflux pumps (EPs) constitutes the leading strategy of bacterial resistance, and it is also a primary driver in the establishment of multidrug resistance (MDR). Extensive efforts are being made to develop antibiotic resistance breakers (ARBs) with the ultimate goal of re-sensitizing bacteria to medications to which they have become unresponsive. EP inhibitors (EPIs) appear to be the principal group of ARBs used to impair the efflux system machinery. Due to the high toxicity of synthetic EPIs, there is a growing interest in natural, safe, and innocuous ones, whereby plant extracts emerge to be excellent candidates. Besides EPIs, further alternatives are being explored including the development of nanoparticle carriers, biologics, and phage therapy, among others. What roles do EPs play in the occurrence of MDR? What weapons do we have to thwart EP-mediated resistance? What are the obstacles to their development? These are some of the core questions addressed in the present review.

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Changing Gly311 to an acidic amino acid in the MATE family protein DTX6 enhances Arabidopsis resistance to the dihydropyridine herbicides

Cited by 23 publications

References 54 publications

Genetic Analysis and Fine Mapping of ZmGHT1 Conferring Glufosinate Herbicide Tolerance in Maize (Zea mays L.)

Genetic Analysis and Fine Mapping of ZmGHT1 Conferring Glufosinate Herbicide Tolerance in Maize (Zea mays L.)

Understanding paraquat resistance mechanisms in Arabidopsis thaliana to facilitate the development of paraquat-resistant crops

What Approaches to Thwart Bacterial Efflux Pumps-Mediated Resistance?

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