Changing expression profiles of long non-coding RNAs, mRNAs and circular RNAs in ethylene glycol-induced kidney calculi rats

Abstract

BackgroundTo explore long non-coding RNA (lncRNA), mRNA and circular RNA (circRNA) expression profiles and their biological functions in the pathogenesis of kidney stones in ethylene glycol-induced urolithiasis rats.ResultsThe expression of 1440 lncRNAs, 2455 mRNAs and 145 circRNAs was altered in the kidneys of urolithiasis rats. GO and KEGG biological pathway analysis were performed to predict the functions of differentially expressed lncRNAs, circRNAs and co-expressed potential targeting genes. Co-expression networks of lncRNA-mRNA and circRNA-miRNA were constructed based on correlation analysis between differentially expressed RNAs. mRNAs coexpressed with lncRNAs were involved in many kidney diseases, e.g., Ephb6 was associated with the reabsorption ability of the kidney. Arl5b was associated with the dynamic changes in the podocyte foot process in podocyte injury. miRNAs co-expressed with circRNAs, such as rno-miR-138-5p and rno-miR-672-5p, have been proven to be functional in hypercalciuria urolithiasis.ConclusionThe expression profile provided a systematic perspective on the potential functions of lncRNAs and circRNAs in the pathogenesis of kidney stones. Differentially expressed lncRNAs and circRNAs might serve as treatment targets for kidney stones.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5052-8) contains supplementary material, which is available to authorized users.

iTRAQ-Based Comparative Proteomics Analysis of Urolithiasis Rats Induced by Ethylene Glycol

“…Urolithiasis is a worldwide problem, and the formation of stones in the kidney stems from many underlying disorders but has not been thoroughly elucidated to date [18]. In our previous study, oral medication of 1% ethylene glycol with drinking water from SD rats for 4 weeks induced CaOx, kidney stones, and renal hypofunction [17]. In the present study, we used the same method to induce rat models into urolithiasis successfully.…”

“…All the experimental protocols were approved by the Ethics Committee for Animal Research of Central South University. The model of kidney stone rat was established as described previously [17]. Briefly, 30 rats were randomly divided into two groups.…”

Effects of Nitroglycerine on Renal Ischemia-Reperfusion Injury in Adult Male Rats

“…A cold blood collection heparin syringe was used to collect blood from the abdominal aorta. After obtaining the plasma, an Auto Analyzer (Selectra-XL, Netherlands) was employed to measure plasma [Cr] and [blood urea nitrogen (BUN)] in the serum and blood samples [21]. A flame photometer (SEAC-20Fp, Italy) was used to measure [Na + ] and [K + ] and an osmometer (Gonotec Osmomat-030, Germany) to measure osmolality [22,23].…”

Therapeutic Effects of Hydralazine on Renal Ischemia-Reperfusion Injury in Rat.

“…(Selectra-XL, Netherlands). 17 The concentration of sodium and potassium were measured by a flame photometer (SEAC-20Fp, Italy). Osmolarity of urine and plasma samples was measured using a Osmometer (Gonotec Osmomat-030, Germany).…”