Changes with maturity in fibre composition and levels of extractable polyphenols in Greek browse: Effects on <i>in vitro</i> gas production and <i>in sacco</i> dry matter degradation

Khazaal, K.; Markantonatos, X.; Nastis, A. S.; Ørskov, E. R.

doi:10.1002/jsfa.2740630210

Cited by 67 publications

(43 citation statements)

References 22 publications

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“…The results obtained in this experiment are in agreement with those reported by Khazaal et al (1993), Blummel and Orskov (1993) and Sileshi et al (1996), who observed a correlation between DM disappearance and gas production but they did not find a significant correlation between the rate of DM degradation (c is ) and the rate of gas production (c gas ). On the other hand, Piva et al (1988), who worked with maize silage, did not obtain a significant (P > 0.05) correlation for the same parameters.…”

supporting

confidence: 92%

“…Maize silage is the only forage containing starch in this experiment. Chemical compositions of forages used in this experiment were consistent with Khazaal et al (1993), Valentin et al (1999), Giger-Riverdin et al (2000).Cumulative gas production profiles from the in vitro fermentation of some forages are shown in Figure 1 and the estimated parameters are given Means within the same row with various superscripts are significantly different, c gas = gas production rate, a gas = gas production (ml) from quickly soluble fraction, b gas = gas production (ml) from insoluble fraction, (a + b) gas = potential gas production, ***P < 0.001, SEM = Standard Error Mean, Sig. = significance level…”

supporting

confidence: 71%

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Comparison of in vitro gas production technique with in situ nylon bag technique to estimate dry matter degradation

Kamalak¹,

Canbolat²,

Gürbüz³

et al. 2005

CZECH J ANIM SCI

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Dry matter (DM) degradation of wheat straw (WS), barley straw (BS), lucerne hay (LH) and maize silage (MS) was determined using two different techniques: (i) in vitro gas production and (ii) nylon bag degradability technique. In vitro gas production and in situ DM disappearance were measured after 3, 6, 12, 24, 48, 72 and 96 hours of incubation. In situ and in vitro DM degradation kinetics was described using the equation y = a + b (1 -e ct ). In all incubations there were significant (P < 0.001) correlations between gas production and in situ DM disappearance or estimated parameters ((a + b) gas and (a + b) is or (a + b) gas and EDMD is ) whereas there were no significant (P > 0.05) correlations between c gas and c is or b gas and b is . Gas production from the insoluble fraction (b) alone explained 98.3% of the variation of EDMD. The inclusion of gas production from the quickly soluble fraction (a) and rate constant (c) of gas production in the regression equation improved the accuracy of EDMD prediction. The correlations between the results of both methodologies seem to be sufficiently strong to predict degradability parameters from gas production parameters. It was concluded that the in vitro gas production technique has good potentiality to predict in situ DM disappearance and some DM degradation parameters.Keywords: in vitro gas production; in situ dry matter degradability; forage 61Czech J. Anim. Sci., 50, 2005 (2): 60-67 Original Paper et al., 1999Cone et al., 1999Cone et al., , 1998Cone et al., , 2002. However, the information available can be considered limited and results somewhat inconsistent. The aim of this study was (I) to determine fermentation kinetics using in vitro gas production and the in situ nylon bag technique and (II) to evaluate the correlations between the fermentation kinetics obtained by two different techniques. MATERIAL AND METHODS Forages and chemical analysisCommercially available and widely used four forages consisting of wheat straw, barley straw, lucerne hay and maize silage were used in this experiment. After drying forage samples were milled through a 1-mm sieve for chemical analysis. DM was determined by drying the samples at 105°C overnight and ashing the samples in a muffle furnace at 525°C for 8 h. Nitrogen (N) content was measured by the Kjeldahl method (AOAC, 1990). Crude protein was calculated as N × 6.25. Neutral detergent fibre (NDF) and acid detergent fibre (ADF) contents were determined by the method of AOAC (1990). Starch content was determined by the method according to MacRea and Armstrong (1968). In vitro gas productionForage samples milled through a 1-mm sieve were incubated in vitro in rumen fluid in calibrated glass syringes following the procedures of Menke and Steingass (1988). Rumen fluid was obtained from three fistulated sheep fed twice daily with a diet containing lucerne hay (60%) and concentrate (40%). Digestion medium was prepared mixing 500 ml of distilled water, 0.1 ml micro-mineral solution, 200 ml buffer solution, 200 ml macro-mi...

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supporting

confidence: 92%

supporting

confidence: 71%

Comparison of in vitro gas production technique with in situ nylon bag technique to estimate dry matter degradation

Kamalak¹,

Canbolat²,

Gürbüz³

et al. 2005

CZECH J ANIM SCI

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“…This is in agreement with Blümmel & Ørskov (1993), Khazaal et al (1993) and Sileshi et al (1996). The highly significant relationship between gas production and DM disappearance after 24 and 96 h incubation suggests that either method could be used to estimate the nutritive value of such tree leaves.…”

Section: Discussionsupporting

confidence: 90%

“…On the other hand, Menke et al (1979) and Menke & Steingass (1988) developed the in vitro gas production technique to evaluate the nutritive value of forages and estimate the rate and extent of DM degradation indirectly using the gas production (CO 2 ) during fermentation. The in situ nylon bag and in vitro gas production technique are well correlated with animal performance (Ørskov, 1989), food intake (Blummel & Ørskov, 1993), microbial protein synthesis (Krishnamoorthy et al, 1991) and in vivo digestibility (Khazaal et al, 1993). More recently researchers have been investigating the relationship between fermentation kinetics of forages obtained by the in situ nylon bag technique and the in vitro gas production technique (Blummel & Ørskov, 1993;Khazaal et al, 1993;Dewhurst et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Comparison between in situ dry matter degradation and in vitro gas production of tannin-containing leaves from four tree species

Kamalak

Canbolat²,

Gürbüz³

2006

SA J. An. Sci.

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Dry matter (DM) degradation of Glycrrhiza glabra L, Arbutus andrachne, Juniperus communis, and Pistica lentiscus was determined using two different techniques: (i) the in vitro gas production and (ii) the in situ nylon bag degradability technique. Samples were incubated in situ and in vitro for 3, 6, 12, 24, 48, 72 and 96 h. In situ and in vitro DM degradation kinetics were described using the equation y = a + b (1 -e ct ). At all incubation times except 3 and 72 h the cumulative gas production of J. communis was significantly lower than that of G. glabra, A. andrachne and P. lentiscus. At 3, 6 and 12 h incubation times the DM disappearance of J. communis was only significantly lower than that of P. lentiscus. At 24 and 48 h incubation times DM disappearance of J. communis was significantly lower than that of A. andrachne and P. lentiscus. There were significant relationships between in vitro gas production and in situ DM disappearance at 24 h and 96 h incubation times. The gas productions at 24 and 96 h incubation explained 51.2 and 52.4% of variation of DM disappearance, respectively. Gas production from the insoluble fraction (b) alone explained 66.4% of the variation of effective DM degradability (EDMD). The inclusion of gas production from quickly soluble fraction (a) and rate constant (c) of gas production in the regression equation did not improve the accuracy of predicting EDMD. It was concluded that in situ DM disappearance parameters of tannin-containing tree leaves such as used in this present study may be predicted from in vitro gas production parameters. _______________________________________________________________________________________

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“…Na avaliação de fatores anti-nutricionais através da produção de gases, nota-se que os efeitos destes fatores na fermentação do rúmen são refletidos pela queda da produção de gases (GETACHEW et al, 1998;SINGH;BHAT;SHARMA, 2001), podendo também interferir na determinação da digestibilidade in vitro (KHAZAAL et al, 1993;CABRAL FILHO et al, 2005 …”

Section: Produção De Gases In Vitrounclassified

"Aspectos nutricionais de compostos fenólicos em ovinos alimentados com leguminosas forrageiras"

Godoy¹

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Changes with maturity in fibre composition and levels of extractable polyphenols in Greek browse: Effects on in vitro gas production and in sacco dry matter degradation

Cited by 67 publications

References 22 publications

Comparison of in vitro gas production technique with in situ nylon bag technique to estimate dry matter degradation

Comparison of in vitro gas production technique with in situ nylon bag technique to estimate dry matter degradation

Comparison between in situ dry matter degradation and in vitro gas production of tannin-containing leaves from four tree species

"Aspectos nutricionais de compostos fenólicos em ovinos alimentados com leguminosas forrageiras"

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