Changes of trehalose content and expression of relative genes during the bioethanol fermentation by<i>Saccharomyces cerevisiae</i>

Yi, Chenfeng; Wang, Fenglian; Dong, Shijun; Li, Hao

doi:10.1139/cjm-2015-0832

Cited by 8 publications

(2 citation statements)

References 44 publications

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“…The intracellular carbohydrates trehalose and glycogen are compatible solutes that resist osmotic pressure across the cytoplasmic membrane. Trehalose is involved in ethanol tolerance in S. cerevisiae (Mahmud et al 2009 ; Wang et al 2013 ; Yi et al 2016 ). The up-regulation of trehalose and glycogen synthesis genes, and the accumulation of trehalose (Fig.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling of Issatchenkia orientalis under ethanol stress

Miao

Xiong

et al. 2018

AMB Expr

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Issatchenkia orientalis, a non-Saccharomyces yeast that can resist a wide variety of environmental stresses, has potential use in winemaking and bioethanol production. Little is known about gene expression or the physiology of I. orientalis under ethanol stress. In this study, high-throughput RNA sequencing was used to investigate the transcriptome profile of I. orientalis in response to ethanol. 502 gene transcripts were differentially expressed, of which 451 were more abundant, and 51 less abundant, in cells subjected to 4 h of ethanol stress (10% v/v). Annotation and statistical analyses suggest that multiple genes involved in ergosterol biosynthesis, trehalose metabolism, and stress response are differentially expressed under these conditions. The up-regulation of molecular chaperones HSP90 and HSP70, and also genes associated with the ubiquitin–proteasome proteolytic pathway suggests that ethanol stress may cause aggregation of misfolded proteins. Finally, ethanol stress in I. orientalis appears to have a nitrogen starvation effect, and many genes involved in nutrient uptake were up-regulated.Electronic supplementary materialThe online version of this article (10.1186/s13568-018-0568-5) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Transcriptome profiling of Issatchenkia orientalis under ethanol stress

Miao

Xiong

et al. 2018

AMB Expr

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“…Trehalose and glycogen levels were both determined using stationary phase cells because the cellular content of both carbohydrates is barely detectable in exponential phase even in wild type cells ( Yi et al 2016 ). As expected, no trehalose was produced in all 5 tps1 Δ strains whereas the wild type strains showed a variety of intracellular trehalose levels ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Characterizing phenotypic diversity of trehalose biosynthesis mutants in multiple wild strains of Saccharomyces cerevisiae

Chen

Smith

Tapia

et al. 2022

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In the yeast Saccharomyces cerevisiae, trehalose-6-phospahte synthase (Tps1) and trehalose-6-phosphate phosphatase (Tps2) are the main proteins catalyzing intracellular trehalose production. In addition to Tps1 and Tps2, two putative regulatory proteins with less clearly defined roles also appear to be involved with trehalose production, Tps3 and Tsl1. While this pathway has been extensively studied in laboratory strains of S. cerevisiae, we sought to examine the phenotypic consequences of disrupting these genes in wild strains. Here we deleted the TPS1, TPS2, TPS3 and TSL1 genes in four wild strains and one laboratory strain for comparison. Although some tested phenotypes were not shared between all strains, deletion of TPS1 abolished intracellular trehalose, caused inability to grow on fermentable carbon sources and resulted in severe sporulation deficiency for all five strains. After examining tps1 mutant strains expressing catalytically inactive variants of Tps1, our results indicate that Tps1, independent of trehalose production, is a key component for yeast survival in response to heat stress, for regulating sporulation, and growth on fermentable sugars. All tps2Δ mutants exhibited growth impairment on non-fermentable carbon sources, whereas variations were observed in trehalose synthesis, thermosensitivity and sporulation efficiency. tps3Δ and tsl1Δ mutants exhibited mild or no phenotypic disparity from their isogenic wild type although double mutants tps3Δ tsl1Δ decreased the amount of intracellular trehalose production in all five strains by 17% - 45%. Altogether, we evaluated, confirmed, and expanded the phenotypic characteristics associated trehalose biosynthesis mutants. We also identified natural phenotypic variants in multiple strains that could be used to genetically dissect the basis of these traits and then develop mechanistic models connecting trehalose metabolism to diverse cellular processes.

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Effects of Lactobacillus plantarum on the ethanol tolerance of Saccharomyces cerevisiae

Liu

et al. 2021

Appl Microbiol Biotechnol

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Changes of trehalose content and expression of relative genes during the bioethanol fermentation bySaccharomyces cerevisiae

Cited by 8 publications

References 44 publications

Transcriptome profiling of Issatchenkia orientalis under ethanol stress

Transcriptome profiling of Issatchenkia orientalis under ethanol stress

Characterizing phenotypic diversity of trehalose biosynthesis mutants in multiple wild strains of Saccharomyces cerevisiae

Effects of Lactobacillus plantarum on the ethanol tolerance of Saccharomyces cerevisiae

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