Changes of the solution pH due to exposure by high-voltage electric pulses

Saulis, Gintautas; Lape, Remigijus; Pranevičiūtė, Rita; Mickevičius, Donatas

doi:10.1016/j.bioelechem.2005.03.001

Cited by 66 publications

(54 citation statements)

References 50 publications

(59 reference statements)

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“…The safety and efficacy of nsPEF have to be examined thoroughly in vivo and in the clinics. Especially, it appears of utmost importance to check for reactions of the electrode that could lead to significant pH changes and solubilization of the electrode material as is the case for micropulses [Saulis et al, 2005], and to improve the reliability and safety of the nanopulsers.…”

Section: Challenges For the Future Of Nanopulse Usementioning

confidence: 99%

Microsecond and nanosecond electric pulses in cancer treatments

Breton¹,

Mir²

2011

Bioelectromagnetics

180

133

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New local treatments based on electromagnetic fields have been developed as non-surgical and minimally invasive treatments of tumors. In particular, short electric pulses can induce important non-thermal changes in cell physiology, especially the permeabilization of the cell membrane. The aim of this review is to summarize the present data on the electroporation-based techniques: electrochemotherapy (ECT), nanosecond pulsed electric fields (nsPEFs), and irreversible electroporation (IRE). ECT is a safe, easy, and efficient technique for the treatment of solid tumors that uses cell-permeabilizing electrical pulses to enhance the activity of a non-permeant (bleomycin) or low permeant (cisplatin) anticancer drug with a very high intrinsic cytotoxicity. The most interesting feature of ECT is its unique ability to selectively kill tumor cells without harming normal surrounding tissue. ECT is already used widely in the clinics in Europe. nsPEFs could represent a drug free, purely electrical cancer therapy. They allow the inhibition of tumor growth, and interestingly, nsPEF can target intracellular organelles. However, many questions remain on the mechanism of action of these pulses. Finally, IRE is a new ablation procedure using pulses that provoke the permanent permeabilization of the cells resulting in their death. This technique does not result in any thermal effect, which is its main advantage in current physical ablation technologies. For both the nsPEF and the IRE, the preservation of the normal tissue, which is characteristic of ECT, has not yet been shown and their safety and efficacy still have to be investigated thoroughly in vivo and in the clinics.

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Section: Challenges For the Future Of Nanopulse Usementioning

confidence: 99%

Microsecond and nanosecond electric pulses in cancer treatments

Breton¹,

Mir²

2011

Bioelectromagnetics

180

133

View full text Add to dashboard Cite

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“…PEF side effects, such as changes in pH at the electrode surface due to electrochemical reactions (Saulis et al 2005) and the occurrence of temperature hot spots due to ohmic heating effects within a nonuniform electric field (Jaeger et al 2009), may contribute to the observed overall enzyme inactivation during PEF processing. As the PEF effect on proteins, enzymes, and other food constituents remains small, possible applications could include the pasteurization of bioactive antimicrobial milk fractions, such as lactoperoxidase, lactoferrin, or immunoglobulins, as well as heat-sensitive vitamin solutions that are destroyed during thermal pasteurization.…”

Section: Impact On Enzymesmentioning

confidence: 99%

Emerging Technologies for Targeted Food Processing

Knorr¹,

Froehling

Jaeger³

et al. 2013

Food Engineering Series

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“…18 Furthermore, hundreds of volts of electricity is commonly used in the traditional gene transfection process, which raises safety concerns to manipulators. Recent study also suggested that the buffer composition and local pH change, creating strongly acidic or basic environment around the cells during electroporation, 19 might result in a destructive effect on cells near the electrodes, and led to low cell survival rate after gene transfection. Therefore, there is a great need to develop a safe and high-efficiency approach for generation of iPSCs.…”

Section: Introductionmentioning

confidence: 99%

Generation of murine induced pluripotent stem cells by using high-density distributed electrodes network

Hwang

et al. 2015

Biomicrofluidics

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This study reports a robust method of gene transfection in a murine primary cell model by using a high-density electrodes network (HDEN). By demonstrating high cell viability after gene transfection and successful expression of transgenes including fluorescent proteins, the HDEN device shows great promise as a solution in which reprogramming efficiency using non-viral induction for generation of murine induced pluripotent stem cells (iPSCs) is optimized. High and steady transgene expression levels in host cells of iPSCs can be demonstrated using this method. Moreover, the HDEN device achieved successful gene transfection with a low voltage of less than 180 V while requiring relatively low cell numbers (less than 1.5 Â 10 4 cells). The results are comparable to current conventional methods, demonstrating a reasonable fluorescent-plasmid transfection rate (42.4% in single transfection and 24.5% in triple transfection) and high cell viability of over 95%. The gene expression levels of each iPSC factor was measured to be over 10-fold higher than that reported in previous studies using a single mouse embryonic fibroblast cell. Our results demonstrate that the generation of iPSCs using HDEN transfection of plasmid DNA may be a feasible and safe alternative to using viral transfection methods in the near future. V C 2015 AIP Publishing LLC. [http://dx

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Changes of the solution pH due to exposure by high-voltage electric pulses

Cited by 66 publications

References 50 publications

Microsecond and nanosecond electric pulses in cancer treatments

Microsecond and nanosecond electric pulses in cancer treatments

Emerging Technologies for Targeted Food Processing

Generation of murine induced pluripotent stem cells by using high-density distributed electrodes network

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