Changes of soluble sugars and ATP content during DMSO droplet freezing and PVS3 droplet vitrification of potato shoot tips

Köpnick, Claudia; Grübe, Marion; Stock, Johanna; Senula, Angelika; Mock, Hans‐Peter; Nagel, Manuela

doi:10.1016/j.cryobiol.2018.09.005

Cited by 23 publications

(22 citation statements)

References 47 publications

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“…Fresh rice roots (0.1 g) were ground with 1 mL of 0.83-M cold perchloric acid (Sigma Aldrich, St. Louis, USA) on ice, and the supernatant was adjusted to a neutral pH with 3-M KOH. The BacTiter-Glo Microbial Cell Viability Assay (Promega, Madison, USA) was used to determine the ATP concentration in a white opaque-walled 96-well microplate (Greiner Bio-One, Frickenhausen, Germany) (Köpnick et al, 2018).…”

Section: Measurement Of Sucrose and Atp Contentmentioning

confidence: 99%

Unearthing the alleviatory mechanisms of hydrogen sulfide in aluminum toxicity in rice

Zhu

Wei

et al. 2022

Plant Physiology and Biochemistry

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Section: Measurement Of Sucrose and Atp Contentmentioning

confidence: 99%

Unearthing the alleviatory mechanisms of hydrogen sulfide in aluminum toxicity in rice

Zhu

Wei

et al. 2022

Plant Physiology and Biochemistry

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“…Rapid rewarming takes place in a third liquid, called recovery solution (RS) after which the meristems are transferred onto an in vitro medium for plant recovery. Since its first report in 2005 (See Box 2 [77]), this cryopreservation protocols has now been successfully applied to 111 plant species according the publications reported in Web of Science (Situation 1 July 2020) among which some very important staple foods are found, such as potato [96][97][98], taro [99], cassava and yam [82], sweet potato [100], and also ornamentals (Pelargonium spp. [101], Nandina domestica [102]), fruit trees (Actinidia chinensis (kiwi fruit)) [103], medicinal plants (Byrsonima intermedia) [104] and conifers (Sequoia sempervirens (redwood) [105]).…”

Section: Droplet Vitrificationmentioning

confidence: 99%

“…Routine cryopreservation of crop collections began only a few decades ago. Currently, about 18 genebanks have cryopreserved crop collections [13,67,81,98,109]. It is estimated that about 100,000 unique accessions of vegetatively propagated and recalcitrant seed crops potentially need long-term conservation through cryopreservation while currently only about 10,000 accessions are cryopreserved [13].…”

Section: Droplet Vitrificationmentioning

confidence: 99%

Challenges and Prospects for the Conservation of Crop Genetic Resources in Field Genebanks, in In Vitro Collections and/or in Liquid Nitrogen

Panis

Nagel

houwe

2020

Plants

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The conservation of crop genetic resources, including their wild relatives, is of utmost importance for the future of mankind. Most crops produce orthodox seeds and can, therefore, be stored in seed genebanks. However, this is not an option for crops and species that produce recalcitrant (non-storable) seeds such as cacao, coffee and avocado, for crops that do not produce seeds at all; therefore, they are inevitably vegetatively propagated such as bananas, or crops that are predominantly clonally propagated as their seeds are not true to type, such as potato, cassava and many fruit trees. Field, in vitro and cryopreserved collections provide an alternative in such cases. In this paper, an overview is given on how to manage and setup a field, in vitro and cryopreserved collections, as well as advantages and associated problems taking into account the practical, financial and safety issues in the long-term. In addition, the need for identification of unique accessions and elimination of duplicates is discussed. The different conservation methods are illustrated with practical examples and experiences from national and international genebanks. Finally, the importance of establishing safe and long-term conservation methods and associated backup possibilities is highlighted in the frame of the global COVID-19 pandemic.

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“…When in vitro plants are excised and shoot tips are exposed to the stressful conditions of cryopreservation, the process triggers massive proliferation and outbreaks of endophytes. The colonisation of these microbes emerges usually around the shoot tips during rewarming and compromises the ability of explants to regrow to a fully developed plant (Köpnick et al 2018;Senula et al 2018;Senula and Keller 2011). An increased number of subcultivations and suboptimal environmental conditions during in vitro plant growth alter the quality of the donor material and provoke endophyte growth after cryopreservation (Keller et al 2011;Scherling et al 2009).…”

Section: Plantsmentioning

confidence: 99%

Microbial occurrence in liquid nitrogen storage tanks: a challenge for cryobanking?

Bajerski

Nagel

Overmann

2021

Appl Microbiol Biotechnol

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Modern biobanks maintain valuable living materials for medical diagnostics, reproduction medicine, and conservation purposes. To guarantee high quality during long-term storage and to avoid metabolic activities, cryostorage is often conducted in the N2 vapour phase or in liquid nitrogen (LN) at temperatures below − 150 °C. One potential risk of cryostorage is microbial cross contamination in the LN storage tanks. The current review summarises data on the occurrence of microorganisms that may compromise the safety and quality of biological materials during long-term storage. We assess the potential for the microbial contamination of LN in storage tanks holding different biological materials based on the detection by culture-based and molecular approaches. The samples themselves, the LN, the human microbiome, and the surrounding environment are possible routes of contamination and can cause cross contaminations via the LN phase. In general, the results showed that LN is typically not the source of major contaminations and only a few studies provided evidence for a risk of microbial cross contamination. So far, culture-based and culture-independent techniques detected only low amounts of microbial cells, indicating that cross contamination may occur at a very low frequency. To further minimise the potential risk of microbial cross contaminations, we recommend reducing the formation of ice crystals in cryotanks that can entrap environmental microorganisms and using sealed or second sample packing. A short survey demonstrated the awareness for microbial contaminations of storage containers among different culture collections. Although most participants consider the risk of cross contaminations in LN storage tanks as low, they prevent potential contaminations by using sealed devices and − 150 °C freezers. It is concluded that the overall risk for cross contaminations in biobanks is relatively low when following standard operating procedures (SOPs). We evaluated the potential sources in detail and summarised our results in a risk assessment spreadsheet which can be used for the quality management of biobanks. Key points • Identification of potential contaminants and their sources in LN storage tanks. • Recommendations to reduce this risk of LN storage tank contamination. • Development of a risk assessment spreadsheet to support quality management.

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Changes of soluble sugars and ATP content during DMSO droplet freezing and PVS3 droplet vitrification of potato shoot tips

Cited by 23 publications

References 47 publications

Unearthing the alleviatory mechanisms of hydrogen sulfide in aluminum toxicity in rice

Unearthing the alleviatory mechanisms of hydrogen sulfide in aluminum toxicity in rice

Challenges and Prospects for the Conservation of Crop Genetic Resources in Field Genebanks, in In Vitro Collections and/or in Liquid Nitrogen

Microbial occurrence in liquid nitrogen storage tanks: a challenge for cryobanking?

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