Changes of Saccharomyces cerevisiae cell membrane components and promotion to ethanol tolerance during the bioethanol fermentation

Dong, Shijun; Yi, Chenfeng; Li, Hao

doi:10.1016/j.biocel.2015.10.025

Cited by 42 publications

(29 citation statements)

References 27 publications

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“…This is contradictory with the data of two previous studies which showed OLE1 down-regulation in yeast cells treated with weak acids, 2,4-D (Viegas et al, 2005) and sorbic acid (Sch€ uller et al, 2004). Prior to this work, several studies showed that OLE1 overexpression enhances ethanol tolerance (Dong et al, 2015;Kajiwara et al, 2000) and that this phenotype correlates with an increase of UI (ratio of unsaturated to saturated FA) (Alexandre et al, 1996;de Kroon et al, 2013). 2), suggesting that there is a general mechanism of response to many stresses, but not specific to a particular type of stress.…”

Section: Discussioncontrasting

confidence: 99%

“…2), suggesting that there is a general mechanism of response to many stresses, but not specific to a particular type of stress. Prior to this work, several studies showed that OLE1 overexpression enhances ethanol tolerance (Dong et al, 2015;Kajiwara et al, 2000) and that this phenotype correlates with an increase of UI (ratio of unsaturated to saturated FA) (Alexandre et al, 1996;de Kroon et al, 2013). Thus, adaptation of cells to new environments may be achieved through disturbing the currently established balance (or homeostasis) of specific physiological parameters.…”

Section: Discussionmentioning

confidence: 99%

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Overexpression of OLE1 enhances stress tolerance and constitutively activates the MAPK HOG pathway in Saccharomyces cerevisiae

Nasution

Lee

Kim

et al. 2016

Biotech & Bioengineering

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OLE1 of Saccharomyces cerevisiae encodes the sole and essential Δ-9 desaturase catalyzing the conversion of saturated to unsaturated fatty acids. Upon ectopic overexpression of OLE1 in S. cerevisiae, significant increases in the membrane oleic acid content were observed. OLE1-overexpressing strains displayed enhanced tolerance to various stresses, better proton efflux, lower membrane permeability, and lessened internal hydrogen peroxide content. The OLE1-mediated enhanced stress tolerance was considerably diminished upon deletion of HOG1, which encodes the mitogen-activated protein kinase (MAPK) Hog1 of the high osmolarity glycerol (HOG) pathway. Furthermore, OLE1 overexpression constitutively activated Hog1, which remained in the cytoplasm. Hog1 activation was accomplished through the MAPK kinase kinase (MAPKKK) Ssk2, but not Ste11 and Ssk22, the other MAPKKKs of the HOG pathway. Despite its cytoplasmic location, activated Hog1 was able to activate the expression of its canonical targets, including CTT1, HSP12, and STL1, and further, the cAMP and stress response elements present in the promoter. OLE1 overexpression neither caused nor relieved endoplasmic reticulum stress. Individually or in combination, the physiological and molecular changes caused by OLE1 overexpression may contribute to enhanced tolerance to various types of stress. Biotechnol. Bioeng. 2017;114: 620-631. © 2016 Wiley Periodicals, Inc.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Overexpression of OLE1 enhances stress tolerance and constitutively activates the MAPK HOG pathway in Saccharomyces cerevisiae

Nasution

Lee

Kim

et al. 2016

Biotech & Bioengineering

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“…As the hypothesis of “homeoviscous adaptation” suggested that variation of fatty-acid composition of membrane phospholipids serves in producing membranes whose lipids have a constant fluidity in the growth process 26 , we supposed that the production of a more fluid membrane in L. acetotolerans F28 was a compensation for the increase in “rigidity” due to the environmental ethanol stress. This behavior is consistent with the ethanol-induced fatty acid changes occurring in Lactobacillus hilgardii 27 and Saccharomyces cerevisiae 28 . In the VPNC cells of L. acetotolerans isolated from spoilage beer, “fatty acid biosynthesis” was down-regulated compared to the normal cells.…”

Section: Discussionsupporting

confidence: 85%

Transcriptome responses of Lactobacillus acetotolerans F28 to a short and long term ethanol stress

Yang

Teng

Zhang

et al. 2017

Sci Rep

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Lactobacillus acetotolerans is a major microbe contributing to the Chinese liquor fermentation with unknown function. It can be grown well in a high concentration of ethanol. RNA sequencing (RNA-seq) was performed on L. acetotolerans F28 growing in 12% ethanol to determine important genetic mechanisms for both a short and long term adaption to this environment. A genome-wide transcriptional analysis revealed that the most important genetic elements for L. acetotolerans F28 grown in ethanol are related to high levels of stress response and fatty acid biosynthesis, and a reduction of amino acid transport and metabolism after both a short and long time stress. The fatty acid methyl ester analyses showed that most fatty acids were increased in L. acetotolerans F28 after exposure to ethanol while the unsaturated fatty acid octadecenoic acid (C18:1) was significantly increased. The increasing unsaturated fatty acid biosynthesis in L. acetotolerans F28 might enhance cell membrane fluidity and protect the cells against high concentration of ethanol. Overall, the transcriptome and functional analysis indicated that the elevated stress response and fatty acid biosynthesis, and the decrease of amino acid transport and metabolism might play important roles for L. acetotolerans F28 to adapt to environmental ethanol.

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“…[5,6] Due to its aforementioned role in cellular biochemistry, ergosterol has been a subject of interest from the viewpoint of increasing ethanol tolerance of S. cerevisiae. [7][8][9] The structure of ergosterol allows its use in microbial conversions to produce similar intermediates as are formed from phytosterols and cholesterol. [10] Although ergosterol is a possible precursor for steroids, [11] fractionation of abundant residual yeast biomass from industrial side-streams to ergosterol and other products has not been studied to a great extent.…”

Section: Introductionmentioning

confidence: 99%

Fractionation process for the protective isolation of ergosterol and trehalose from microbial biomass

Pastinen

Nyyssölä

Pihlajaniemi

et al. 2017

Process Biochemistry

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A new process is described for the two phase extraction of ergosterol and trehalose from microbial biomass. Baker's yeast was used as a model organism to develop the method, which was then applied for extracting 13 oleaginous microbes. Major findings of the study were that the ergosterol content was not dependent on intracellular oil content and that 1-butanol and alkaline pH were needed to protect ergosterol. Saponification for 3−4 hours at 85−100 ºC followed by extraction of the reaction mixture with toluene gave the maximal ergosterol yield.Trehalose was stable at this temperature and remained in water solution, but the maximal yield was obtained after a shorter reaction time at lower alkalinity. Although trehalose alone is stable at alkaline pH, extraction yields of trehalose from yeast decreased with increasing alkalinity.This finding led us to propose a two-step process in which trehalose is separated in the first step and ergosterol in the second. The possibility to apply this method to fractionate oleaginous microbes in process scale is discussed from technical viewpoints.

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Changes of Saccharomyces cerevisiae cell membrane components and promotion to ethanol tolerance during the bioethanol fermentation

Cited by 42 publications

References 27 publications

Overexpression of OLE1 enhances stress tolerance and constitutively activates the MAPK HOG pathway in Saccharomyces cerevisiae

Overexpression of OLE1 enhances stress tolerance and constitutively activates the MAPK HOG pathway in Saccharomyces cerevisiae

Transcriptome responses of Lactobacillus acetotolerans F28 to a short and long term ethanol stress

Fractionation process for the protective isolation of ergosterol and trehalose from microbial biomass

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