Changes of primary sequence and secondary structure proximal to the 5? end of the stop codon substantially increases the expression of the variable region of an antibody in E. coli
Abstract:The sequence context at the 5' end of the stop codon may influence the efficiency of termination and translation. To increase the expression of a designed variable region of an antibody (named as VH5) against tumor necrosis factor alpha (TNFalpha), two nucleotides (TC) at 25 and 26 nucleotides (nt) upstream of termination codon were substituted with AG, respectively. The free energy of 70 nt (arbitrarily defined from the 32 nt upstream of termination codon to 38 nt downstream) was changed from -13.5 kcal mol-1… Show more
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