Changes of polyamine pattern in digestive glands of mussel Mytilus galloprovincialis under exposure to cadmium

Kournoutou, Georgia G.; Pytharopoulou, Sofia; Leotsinidis, Michalis; Kalpaxis, Dimitrios L.

doi:10.1016/j.cbpc.2014.05.005

Cited by 5 publications

(8 citation statements)

References 58 publications

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“…It is the precursor of both spermidine and γ-aminobutyric acid [42], whereby the latter was putatively identified in our spectrum. Interestingly, it was shown to be involved in the response to oxidative stress induced by cadmium exposure in Mytillus galloprovincialis, suggesting a potential role in oxidative stress response [46], which can be interesting in the context of ecotoxicological studies. Gamma-aminobutyric acid (GABA) is another biogenic amine, which can be synthesized from putrescine as mentioned earlier, or from glutamate.…”

Section: Informative Potential Of Nmr Based Metabolomics In D Polymorphamentioning

confidence: 99%

The Zebra Mussel (Dreissena polymorpha) as a Model Organism for Ecotoxicological Studies: A Prior 1H NMR Spectrum Interpretation of a Whole Body Extract for Metabolism Monitoring

et al. 2020

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The zebra mussel (Dreissena polymorpha) represents a useful reference organism for the ecotoxicological study of inland waters, especially for the characterization of the disturbances induced by human activities. A nuclear magnetic resonance (NMR)-based metabolomic approach was developed on this species. The investigation of its informative potential required the prior interpretation of a reference 1H NMR spectrum of a lipid-free zebra mussel extract. After the extraction of polar metabolites from a pool of whole-body D. polymorpha powder, the resulting highly complex 1D 1H NMR spectrum was interpreted and annotated through the analysis of the corresponding 2D homonuclear and heteronuclear NMR spectra. The spectrum interpretation was completed and validated by means of sample spiking with 24 commercial compounds. Among the 238 detected 1H signals, 53% were assigned, resulting in the identification of 37 metabolites with certainty or high confidence, while 5 metabolites were only putatively identified. The description of such a reference spectrum and its annotation are expected to speed up future analyses and interpretations of NMR-based metabolomic studies on D. polymorpha and to facilitate further explorations of the impact of environmental changes on its physiological state, more particularly in the context of large-scale ecological and ecotoxicological studies.

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Section: Informative Potential Of Nmr Based Metabolomics In D Polymorphamentioning

confidence: 99%

The Zebra Mussel (Dreissena polymorpha) as a Model Organism for Ecotoxicological Studies: A Prior 1H NMR Spectrum Interpretation of a Whole Body Extract for Metabolism Monitoring

et al. 2020

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“…Lysosomal membrane stability (as NRRT50) is considered as a sensitive pollution biomarker (Guidi et al, 2010;Regoli et al, 2004;Viarengo et al, 2007). Particularly, exposures to domiciliary waste (Parolini, Binelli, & Provini, 2011;Toufexi et al, 2013;Tsarpali & Dailianis, 2012) as well as to metals (Domouhtsidou, Dailianis, Kaloyianni, & Dimitriadis, 2004;Kournoutou, Pytharopoulou, Leotsinidis, & Kalpaxis, 2014) destabilize lysosomal membrane in bivalve hemocytes. We detected lower NRRT50 in hemocytes of S2 bivalves than in the other sites in March and July, with the clearest effect in July, in coincidence with a marked increase in ROS level and with the highest records of OM content and nitrate concentration, and highest copper concentration for this site.…”

Section: Discussionmentioning

confidence: 99%

Long‐ term effects of water quality on the freshwater bivalve Diplodon chilensis (Unionida: Hyriidae) caged at different sites in a North Patagonian river (Argentina)

et al. 2020

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Water quality was monitored along an Andean river of global importance using the freshwater bivalve Diplodon chilensis as sentinel species. Bivalves were placed in cages at three sites (S1–3) in the Chimehuin River in order to evaluate the long‐term effects of a trout hatchery (S2) and the open dump and sewage treatment plant of a nearby city (S3). Water samples and bivalves were collected at 0, 3, 6, 9 and 12 months, from S3, S2 and from a reference site upstream (S1), and physicochemical parameters and the biological response of the caged bivalves were studied. ROS production, antioxidant response, oxidative damage, energy status and morphometric ratios were included as response biomarkers. Most of biomarkers showed site and time dependence including bivalves transplanted at S1, revealing natural variability. Both anthropogenic perturbed sites (S2 and S3) showed differences in the exposed bivalves with respect to the reference site (S1) in their biomarker responses after 9 and 12 months of exposure (July and September, respectively). Multivariate analysis showed alterations in hemocytes (ROS, TOSC and NRRT50), in energy balance in digestive gland (energy and DGF) and in the detoxification response (GST) and GSH values in gill when bivalves were exposed to hatchery waste; whereas metal and bacterial pollution (S3 in July) caused GSH increase and a reduction of lysosomal damage in hemocytes. Results show that changes in the water quality of Chimehuin River due to the anthropogenic impact can be detected using the biomarkers analysed on D. chilensis, being a useful tool for studies of long‐term monitories.

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“…In planarians and earthworms, the synthesis of Put is stimulated during regeneration, and under temperature and osmotic stresses, the levels of Put and Spd in C. elegans, planarians, and earthworms are temporarily increased (Hamana et al, 1995). The levels of polyamines fluctuate under Cd + stress in mussels (Kournoutou et al, 2014). These results suggested that polyamines may be important regulatory molecules in stress response.…”

Section: Introductionmentioning

confidence: 96%

“…The level of polyamines in organisms is strictly regulated. Environmental changes, tumors, oxidative stress, pathogen infection, or injury will cause changes in the polyamine levels (Wickström, 1991;Cipolla et al, 1993;Kournoutou et al, 2014). Polyamines are not only involved in cell growth, gene regulation, differentiation, and development but also have recently been found to alter the inflammatory response in vitro and immunity (Yuan et al, 2001;Pirinen et al, 2007;Choi and Park, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…In invertebrates, polyamines were first observed in Cionia intestinalisin in 1954 (Ackermann and Janka, 1954). Subsequently, polyamines have been detected in sea urchin, sea cucumber, sea squirt, oysters, short-necked clam, C. elegans, planarians, earthworms, mussel, and Bombyx mori (Hamana et al, 1991(Hamana et al, , 1995Kournoutou et al, 2014;Yerra et al, 2016). In planarians and earthworms, the synthesis of Put is stimulated during regeneration, and under temperature and osmotic stresses, the levels of Put and Spd in C. elegans, planarians, and earthworms are temporarily increased (Hamana et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

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Polyamine Putrescine Regulates Oxidative Stress and Autophagy of Hemocytes Induced by Lipopolysaccharides in Pearl Oyster Pinctada fucata martensii

et al. 2021

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The polyamine putrescine (Put) is a ubiquitous small cationic amine. It plays an essential role in controlling the innate immune response. However, little is known about its function in mollusks. In this study, the Put content was observed to increase in the serum of pearl oyster Pinctada fucata martensii after 6 and 24 h of lipopolysaccharide (LPS) stimulation. Activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) increased, and nitric oxide synthase was downregulated in the Put group (i.e., combined treatment with Put and LPS) compared with that in the LPS group (i.e., combined treatment with phosphate-buffered saline and LPS). Furthermore, activities of alkaline phosphatase and acid phosphatase were inhibited after 6 h of LPS stimulation. The expression levels of the nuclear factor kappa B, IκB kinase, Janus kinase, and signal transducer and activator of transcription proteins genes were all significantly suppressed at 12 and 24 h in the Put group. Pseudomonas aeruginosa and Bacillus subtilis grew better after being incubated with the serum from the Put group than that from the LPS group. Additionally, the Put treatment remarkably inhibited the autophagy of hemocytes mediated by the AMP-activated protein kinase-mammalian target of rapamycin-Beclin-1 pathway. This study demonstrated that Put can effectively inhibit the inflammatory response induced by LPS in pearl oysters. These results provide useful information for further exploration of the immunoregulatory functions of polyamines in bivalves and contribute to the development of immunosuppressive agents.

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Changes of polyamine pattern in digestive glands of mussel Mytilus galloprovincialis under exposure to cadmium

Cited by 5 publications

References 58 publications

The Zebra Mussel (Dreissena polymorpha) as a Model Organism for Ecotoxicological Studies: A Prior 1H NMR Spectrum Interpretation of a Whole Body Extract for Metabolism Monitoring

The Zebra Mussel (Dreissena polymorpha) as a Model Organism for Ecotoxicological Studies: A Prior 1H NMR Spectrum Interpretation of a Whole Body Extract for Metabolism Monitoring

Long‐ term effects of water quality on the freshwater bivalve Diplodon chilensis (Unionida: Hyriidae) caged at different sites in a North Patagonian river (Argentina)

Polyamine Putrescine Regulates Oxidative Stress and Autophagy of Hemocytes Induced by Lipopolysaccharides in Pearl Oyster Pinctada fucata martensii

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