Changes of occludin expression in intestinal mucosa after burn in rats

Shao, Longquan; Huang, Qiren; He, Ming; Zeng, Huihong; Li-dan, Wan; Zhu, Qing

doi:10.1016/j.burns.2005.05.005

Cited by 9 publications

(4 citation statements)

References 20 publications

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“…This is similar to the previous study revealing that both decreased expression and reorganization of intestinal tight junction proteins ZO-1 and occludin are induced in balb/c mice undergoing a 30% TBSA steam burn [34]. However, it has been reported that both mRNA and protein expression of occludin are up-regulated in Wistar rats inflicted with 30% TBSA scald injury [35]. Thus, it is suggested that the reorganization of tight junction proteins is involved in the burn-induced intestinal barrier disruption, whereas the role of altered expression of tight junction proteins is still controversial, and need to be defined.…”

Section: Discussionsupporting

confidence: 91%

Myosin Light Chain Kinase Mediates Intestinal Barrier Disruption following Burn Injury

Chen

Wang

et al. 2012

PLoS ONE

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BackgroundSevere burn injury results in the loss of intestinal barrier function, however, the underlying mechanism remains unclear. Myosin light chain (MLC) phosphorylation mediated by MLC kinase (MLCK) is critical to the pathophysiological regulation of intestinal barrier function. We hypothesized that the MLCK-dependent MLC phosphorylation mediates the regulation of intestinal barrier function following burn injury, and that MLCK inhibition attenuates the burn-induced intestinal barrier disfunction.Methodology/Principal FindingsMale balb/c mice were assigned randomly to either sham burn (control) or 30% total body surface area (TBSA) full thickness burn without or with intraperitoneal injection of ML-9 (2 mg/kg), an MLCK inhibitor. In vivo intestinal permeability to fluorescein isothiocyanate (FITC)-dextran was measured. Intestinal mucosa injury was assessed histologically. Tight junction proteins ZO-1, occludin and claudin-1 was analyzed by immunofluorescent assay. Expression of MLCK and phosphorylated MLC in ileal mucosa was assessed by Western blot. Intestinal permeability was increased significantly after burn injury, which was accompanied by mucosa injury, tight junction protein alterations, and increase of both MLCK and MLC phosphorylation. Treatment with ML-9 attenuated the burn-caused increase of intestinal permeability, mucosa injury, tight junction protein alterations, and decreased MLC phosphorylation, but not MLCK expression.Conclusions/SignificanceThe MLCK-dependent MLC phosphorylation mediates intestinal epithelial barrier dysfunction after severe burn injury. It is suggested that MLCK-dependent MLC phosphorylation may be a critical target for the therapeutic treatment of intestinal epithelial barrier disruption after severe burn injury.

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Section: Discussionsupporting

confidence: 91%

Myosin Light Chain Kinase Mediates Intestinal Barrier Disruption following Burn Injury

Chen

Wang

et al. 2012

PLoS ONE

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“…Previous studies have shown that TJ was disrupted by several intestinal mucosal damages caused by inflammatory bowel disease, ischemia-reperfusion of ileum, burn, and so on [15][16][17]. CPT-11 also produces characteristic intestinal mucosal damage by inducing apoptosis [18].…”

Section: Discussionmentioning

confidence: 99%

Irinotecan Injures Tight Junction and Causes Bacterial Translocation in Rat

Nakao

Kurita

Komatsu

et al. 2012

Journal of Surgical Research

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“…64 Intestinal ischemia results in intestinal mucosal congestion, widening of intercellular space, shortening of villi, and even loss of the whole mucosal layer to form an ulcer, causing increased intestinal permeability, bacterial translocation, and severe intestinal infections. 65 This destruction of the intestinal barrier was accompanied by an actin depolymerization factor/cofilin activation, an increase in G-actin, and a decrease in F-actin, ZO-1, and claudin-3. 66 After tissue ischemia, the most fundamental treatment is to restore tissue blood perfusion as soon as possible.…”

Section: ■ Pathogenesis Of Intestinal Barrier Injurymentioning

confidence: 99%

“…During an ischemic shock or stress response, the body redistributes blood in the whole body and reduces the gastrointestinal blood flow significantly . Intestinal ischemia results in intestinal mucosal congestion, widening of intercellular space, shortening of villi, and even loss of the whole mucosal layer to form an ulcer, causing increased intestinal permeability, bacterial translocation, and severe intestinal infections . This destruction of the intestinal barrier was accompanied by an actin depolymerization factor/cofilin activation, an increase in G-actin, and a decrease in F-actin, ZO-1, and claudin-3 …”

Section: Pathogenesis Of Intestinal Barrier Injurymentioning

confidence: 99%

Protective Effects of Natural Polysaccharides on Intestinal Barrier Injury: A Review

Huo

Sun

et al. 2022

J. Agric. Food Chem.

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Owing to their minimal side effects and effective protection from oxidative stress, inflammation, and malignant growth, natural polysaccharides (NPs) are a potential adjuvant therapy for several diseases caused by intestinal barrier injury (IBI). More studies are accumulating on the protective effects of NPs with respect to IBI, but the underlying mechanisms remain unclear. Thus, this review aims to represent current studies that investigate the protective effects of NPs on IBI by directly maintaining intestinal epithelial barrier integrity (inhibiting oxidative stress, regulating inflammatory cytokine expression, and increasing tight junction protein expression) and indirectly regulating intestinal immunity and microbiota. Furthermore, the mechanisms underlying IBI development are briefly introduced, and the structure–activity relationships of polysaccharides with intestinal barrier protection effects are discussed. Potential developments and challenges associated with NPs exhibiting protective effects against IBI have also been highlighted to guide the application of NPs in the treatment of intestinal diseases caused by IBI.

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Changes of occludin expression in intestinal mucosa after burn in rats

Cited by 9 publications

References 20 publications

Myosin Light Chain Kinase Mediates Intestinal Barrier Disruption following Burn Injury

Myosin Light Chain Kinase Mediates Intestinal Barrier Disruption following Burn Injury

Irinotecan Injures Tight Junction and Causes Bacterial Translocation in Rat

Protective Effects of Natural Polysaccharides on Intestinal Barrier Injury: A Review

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