Changes of monoterpenes in stem of Chinese white pine (Pinus armandi) saplings following treatment with Methyl Jasmonate

Pham, Thanh; Chen, Hui; Zhang, Ranran; Dai, Lulu; Vu, T. Q. T.

doi:10.2478/fsmu-2014-0006

Cited by 4 publications

(7 citation statements)

References 29 publications

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“…Plants treated with MeJA exhibited an increase in the amounts of terpenes such as α-pinene, β-pinene, limonene, β-phellandrene, camphene, calarene, myrcene and even (+)-3-carene. Terpene accumulation following MeJA treatment has been previously described to occur in Norway spruce ( Franceschi et al, 2002 ; Martin et al, 2002 ; Erbilgin et al, 2006 ; Zulak et al, 2009 ; Zhao et al, 2010 ), and also in other conifer species (e.g., Hudgins et al, 2003 ; Erbilgin and Colgan, 2012 ; Pham et al, 2014 ; Lundborg et al, 2019 ; Chen R. et al, 2020 ). In line with our findings, Zhao et al (2010) and Lundborg et al (2016b) found that α-pinene and limonene respond strongly to treatment with MeJA in Norway spruce.…”

Section: Discussionmentioning

confidence: 92%

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Variation in Methyl Jasmonate-Induced Defense Among Norway Spruce Clones and Trade-Offs in Resistance Against a Fungal and an Insect Pest

et al. 2021

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An essential component of plant defense is the change that occurs from a constitutive to an induced state following damage or infection. Exogenous application of the plant hormone methyl jasmonate (MeJA) has shown great potential to be used as a defense inducer prior to pest exposure, and could be used as a plant protection measure. Here, we examined (1) the importance of MeJA-mediated induction for Norway spruce (Picea abies) resistance against damage by the pine weevil Hylobius abietis, which poses a threat to seedling survival, and infection by the spruce bark beetle-associated blue-stain fungus Endoconidiophora polonica, (2) genotypic variation in MeJA-induced defense (terpene chemistry), and (3) correlations among resistance to each pest. In a semi-field experiment, we exposed rooted-cuttings from nine different Norway spruce clones to insect damage and fungal infection separately. Plants were treated with 0, 25, or 50 mM MeJA, and planted in blocks where only pine weevils were released, or in a separate block in which plants were fungus-inoculated or not (control group). As measures of resistance, stem area debarked and fungal lesion lengths were assessed, and as a measure of defensive capacity, terpene chemistry was examined. We found that MeJA treatment increased resistance to H. abietis and E. polonica, but effects varied with clone. Norway spruce clones that exhibited high constitutive resistance did not show large changes in area debarked or lesion length when MeJA-treated, and vice versa. Moreover, insect damage negatively correlated with fungal infection. Clones receiving little pine weevil damage experienced larger lesion lengths, and vice versa, both in the constitutive and induced states. Changes in absolute terpene concentrations occurred with MeJA treatment (but not on proportional terpene concentrations), however, variation in chemistry was mostly explained by differences between clones. We conclude that MeJA can enhance protection against H. abietis and E. polonica, but the extent of protection will depend on the importance of constitutive and induced resistance for the Norway spruce clone in question. Trade-offs among resistances do not necessarily hinder the use of MeJA, as clones that are constitutively more resistant to either pest, should show greater MeJA-induced resistance against the other.

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Section: Discussionmentioning

confidence: 92%

“…Samples of approx. 1.5 cm 2 in size were chopped into smaller pieces (following standard protocol as described in Persson et al, 1993;Axelsson et al, 2020), and each was immediately placed (using tweezers) in a 2-ml glass vial with solvent. Phloem samples were extracted in 0.5 mL n-hexane (VWR, Ref no.…”

Section: Chemical Analysesmentioning

confidence: 99%

Variation in Methyl Jasmonate-Induced Defense Among Norway Spruce Clones and Trade-Offs in Resistance Against a Fungal and an Insect Pest

et al. 2021

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“…It also induces formation of traumatic resin ducts and polyphenolic parenchyma cells (Hudgins et al 2004). Partly because of their potential applications in pest control, physiological and chemical responses to MeJA have been widely studied in both conifer seedlings (Fäldt et al 2003; Gould et al 2008; Heijari et al 2005; Moreira et al 2009, 2012; Pham et al 2014; Zeneli et al 2006) and trees (Erbilgin et al 2006; Fäldt et al 2003; Schiebe et al 2012; Zhao et al 2011). …”

Section: Introductionmentioning

confidence: 99%

Methyl Jasmonate-Induced Monoterpenes in Scots Pine and Norway Spruce Tissues Affect Pine Weevil Orientation

et al. 2016

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In large parts of Europe, insecticide-free measures for protecting conifer plants are desired to suppress damage by the pine weevil Hylobius abietis (L.). Treatment with methyl jasmonate (MeJA), a chemical elicitor already used in crop production, may enhance expression of chemical defenses in seedlings in conifer regenerations. However, in a previous experiment, MeJA treatment resulted in substantially better field protection for Scots pine (Pinus sylvestris L.) than for Norway spruce (Picea abies (L.) Karst.). Hypothesizing that the variations may be at least due partly to volatiles released by MeJA-treated seedlings and their effects on pine weevil orientation, we examined tissue extracts of seedlings (from the same batches as previously used) by two-dimensional GC-MS. We found that the MeJA treatment increased contents of the monoterpene (−)-β-pinene in phloem (the weevil’s main target tissue) of both tree species, however, the (−)-β-pinene/(−)-α-pinene ratio increased more in the phloem of P. sylvestris. We also tested the attractiveness of individual monoterpenes found in conifer tissues (needles and phloem) for pine weevils using an arena with traps baited with single-substance dispensers and pine twigs. Trap catches were reduced when the pine material was combined with a dispenser releasing (−)-β-pinene, (+)-3-carene, (−)-bornyl acetate or 1,8-cineole. However, (−)-α-pinene did not have this effect. Thus, the greater field protection of MeJA-treated P. sylvestris seedlings may be due to the selective induction of increases in contents of the deterrent (−)-β-pinene, in contrast to strong increases in both non-deterrent (−)-α-pinene and the deterrent (−)-β-pinene in P. abies seedlings.Electronic supplementary materialThe online version of this article (doi:10.1007/s10886-016-0790-z) contains supplementary material, which is available to authorized users.

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“…However, the origins and functions of myrtenol, myrtanol and myrtenal remain unclear. Myrtenol, myrtanol and myrtenal have been detected only in newly invaded female D. armandi , and have been detected in P. armandi in previous studies . To eliminate the effects of different test methods, the presence of myrtenol, myrtanol and myrtenal in healthy and infected P. armandi , and in both newly emerged and emerged D. armandi was investigated using a uniform approach.…”

Section: Introductionmentioning

confidence: 99%

“…22,23 The identification of host and bark beetle volatiles is usually performed using gas chromatographic and mass spectrometry (GC-MS) analyses. To investigate these compounds in relation to D. armandi, volatiles from the needles, resin and phloem of healthy and infected P. armandi [24][25][26][27][28] ; the air surrounding attacked and healthy P. armandi logs 5 ; and the hindgut extracts of female (mated and unmated) 4,5,29 and male 29 D. armandi and their frass 27,29 have been analysed by GC-MS in recent years, and some terpenes, sesquiterpenes and alkenols have been identified. However, because these studies used different test methods and had different emphases, the presence of some-micro volatiles has not been uniformly analysed.…”

Section: Introductionmentioning

confidence: 99%

Presence and roles of myrtenol, myrtanol and myrtenal in Dendroctonus armandi (Coleoptera: Curculionidae: Scolytinae) and Pinus armandi (Pinales: Pinaceae: Pinoideae)

Zhao

Liu

Sun

et al. 2019

Pest Management Science

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BACKGROUND Insect pheromones and host volatiles are important for pest control due to their high efficiency and low potential for environmental pollution. The functions of myrtenol, myrtanol and myrtenal in pest–host interactions are unknown. This study aimed to determine the presence of myrtenol, myrtanol and myrtenal in newly emerged and emerged stages of Dendroctonus armandi, and in infected and healthy Pinus armandi, and to identify their roles in tree protection and pest management based on electroantennography (EAG), Y‐tube and toxicity experiments. RESULTS Gas chromatographic and mass spectrometry (GC–MS) analyses, EAG, Y‐tube and toxicity assays revealed the following: (1) myrtenol was found in P. armandi phloem and did not exhibit significant toxicity towards D. armandi; (2) myrtanol was produced by infected P. armandi after D. armandi invasion and had significant toxicity towards D. armandi, especially females; and (3) myrtenal might represent an aggregation pheromone produced by female D. armandi to exert aggregation effects on other females, to help them overcome the resistance of P. armandi jointly and ensure a successful invasion, females remained in an aggregation state from leaving the host to mating in a new host. CONCLUSION These findings suggest that myrtanol as a repellent has potential for the protection of P. armandi and that myrtenal could be used to trap and disorient D. armandi. © 2019 Society of Chemical Industry

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Changes of monoterpenes in stem of Chinese white pine (Pinus armandi) saplings following treatment with Methyl Jasmonate

Cited by 4 publications

References 29 publications

Variation in Methyl Jasmonate-Induced Defense Among Norway Spruce Clones and Trade-Offs in Resistance Against a Fungal and an Insect Pest

Variation in Methyl Jasmonate-Induced Defense Among Norway Spruce Clones and Trade-Offs in Resistance Against a Fungal and an Insect Pest

Methyl Jasmonate-Induced Monoterpenes in Scots Pine and Norway Spruce Tissues Affect Pine Weevil Orientation

Presence and roles of myrtenol, myrtanol and myrtenal in Dendroctonus armandi (Coleoptera: Curculionidae: Scolytinae) and Pinus armandi (Pinales: Pinaceae: Pinoideae)

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