2005

DOI: 10.3892/or.13.4.607

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Changes of histological and biological features by serial passages in a human adenoid cystic carcinoma line transplantable in nude mice

Susumu Hashitani

¹

,

Kazuma Noguchi²,

et al.

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Cited by 6 publications

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“…Similar findings have been observed by our group and others suggesting that cells with chromosomal translocations cannot be sustained in prolonged propagation. [19, 22-24, 29, 30] …”

Section: Discussionmentioning

confidence: 99%

Development and characterization of salivary adenoid cystic carcinoma cell line

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²

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et al. 2014

Oral Oncology

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Objective To develop in-vitro adenoid cystic carcinoma cell line as a surrogate for functional studies. Materials and Methods Cells obtained from a primary ACC of the base of tongue were cultivated in-vitro and immortalized with h-TERT. Morphologic, cytogenetic and functional studies were performed. Results Tumor cells were verified by positive reactions to keratin and smooth muscle actin and phenotypic cellular and nuclear features. In-vitro cell growth and colony formation assay supported their tumor nature. Conclusion We authenticated an ACC cell line with hybrid epithelial-myoepithelial feature as a resource for functional experimentation.

“…Similar findings have been observed by our group and others suggesting that cells with chromosomal translocations cannot be sustained in prolonged propagation. [19, 22-24, 29, 30] …”

Section: Discussionmentioning

confidence: 99%

Development and characterization of salivary adenoid cystic carcinoma cell line

¹

,

²

,

³

et al. 2014

Oral Oncology

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Objective To develop in-vitro adenoid cystic carcinoma cell line as a surrogate for functional studies. Materials and Methods Cells obtained from a primary ACC of the base of tongue were cultivated in-vitro and immortalized with h-TERT. Morphologic, cytogenetic and functional studies were performed. Results Tumor cells were verified by positive reactions to keratin and smooth muscle actin and phenotypic cellular and nuclear features. In-vitro cell growth and colony formation assay supported their tumor nature. Conclusion We authenticated an ACC cell line with hybrid epithelial-myoepithelial feature as a resource for functional experimentation.

“…Similar heterogeneity was seen in the corresponding xenograft, a phenomenon not typically described in cell lines. Unlike xenograft models of ovarian cancer 22 and adenoid cyst carcinoma, 23 the histology and morphology of most E/GEJ PTXG models remained relatively stable even up to late passages (P5 and beyond). Beyond these late passages areas of necrosis and cellular debris notably increased.…”

Section: Discussionmentioning

confidence: 99%

Primary esophageal and gastro-esophageal junction cancer xenograft models: clinicopathological features and engraftment

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²

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et al. 2013

Laboratory Investigation

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There are very few xenograft models available for the study of esophageal (E) and gastro-esophageal junction (GEJ) cancer. Using a NOD/SCID model, we implanted 90 primary E and GEJ tumors resected from patients and six endoscopic biopsy specimens. Of 69 resected tumors with histologically confirmed viable adenocarcinoma or squamous cell carcinoma, 22 (32%) was engrafted. One of 11 tumors, considered to have had a complete pathological response to neo-adjuvant chemo-radiation, also engrafted. Of the 23 patients whose tumors were engrafted, 65% were male; 30% were early stage while 70% were late stage; 22% received neo-adjuvant chemo-radiation; 61% were GEJ cancers. Engraftment occurred in 18/54 (33%) adenocarcinomas and 5/16 (31%) squamous cell carcinomas. Small endoscopic biopsy tissue had a 50% (3/6) engraftment rate. Of the factors analyzed, pretreatment with chemo-radiation and well/moderate differentiation showed significantly lower correlation with engraftment (Po0.05). In the subset of patients who did not receive neo-adjuvant chemo-radiation, 18/41 (44%) engrafted compared with those with pretreatment where 5/29 (17%, P ¼ 0.02) engrafted. Primary xenograft lines may be continued through 4-12 passages. Xenografts maintained similar histology and morphological characteristics with only minor variations even after multiple passaging in most instances.

“…It is also possible that genetic alteration leading to increases in metastatic potential spontaneously occurs in vivo or in vitro, as ACCS-M GFP was established from a primary tumor and not from metastatic lymph nodes and was passaged in vitro. A similar transformation of an AdCC cell line has been reported during serial passage in vitro (18). Therefore, the identification of the critical genes related to metastasis will be important for understanding AdCC metastasis.…”

Section: Discussionmentioning

confidence: 98%

Involvement of epithelial-mesenchymal transition in adenoid cystic carcinoma metastasis

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et al. 2011

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Abstract. The high frequencies of recurrence and distant metastasis of adenoid cystic carcinoma (AdCC) are significant obstacles for the long-term cure of patients with AdCC and emphasize the need for better understanding of the biological factors associated with these outcomes. To identify proteins that mediate AdCC metastasis, we established three AdCC cell lines expressing green fluorescent protein (GFP) from the ACCS cell line using orthotopic transplantation and in vivo selection in nude mice: Parental ACCS-GFP, highly tumorigenic ACCS-T GFP and metastatic ACCS-M GFP. ACCS-GFP and ACCS-M GFP were subjected to DNA microarray analysis and the results were used for data mining studies. DNA microarray analysis revealed significantly altered biological processes in the ACC-M GFP cells, including events related to cell adhesion (three categories) and signaling (three categories). In particular, a significant down-regulation of cell adhesion molecules, such as cadherins and integrin subunits was observed. The loss of E-cadherin and integrins and the gain of vimentin in ACCS-M GFP cells were confirmed by immunoblotting. These results suggest that epithelial-mesenchymal transition (EMT) is a putative event in AdCC metastasis that induces tumor cell dissemination from the primary tumor site. In summary, in this study we established a useful nude mouse metastasis model which will enable further AdCC metastasis research and clinical treatment trials and we also provide evidence that EMT is significantly involved in the AdCC metastatic process.

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