Changes of hepatic lipid and fatty acid profiles in rats administered iron-deficient diet and ethanol

Hirosue, Toshiko; Hosogai, Yutaro

doi:10.1016/0278-6915(93)90048-4

Cited by 7 publications

(7 citation statements)

References 16 publications

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“…The most striking and unexpected finding of this study was the profound reduction in hepatic steatosis and hepatic triglyceride levels associated with iron supplementation in MCD animals. While the mechanisms underlying this reduction in hepatic lipid are uncertain, it is clear from the literature that iron can influence lipid metabolism (42–46). A marked reduction in hepatic triglyceride levels has been reported in iron‐overloaded rats (42), while elevated hepatic lipids have been reported in rats that are iron deficient (43).…”

Section: Discussionmentioning

confidence: 99%

“…While the mechanisms underlying this reduction in hepatic lipid are uncertain, it is clear from the literature that iron can influence lipid metabolism (42–46). A marked reduction in hepatic triglyceride levels has been reported in iron‐overloaded rats (42), while elevated hepatic lipids have been reported in rats that are iron deficient (43). Several additional studies have documented perturbations in plasma lipid levels in rodents with iron overload (42, 44–46), further supporting the view that iron can modulate lipid metabolism.…”

Section: Discussionmentioning

confidence: 99%

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Effects of iron overload in a rat nutritional model of non‐alcoholic fatty liver disease

Kirsch

Sijtsema²,

Tlali³

et al. 2006

Liver International

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Background/Aims: This study sought to determine whether excess hepatic iron potentiates liver injury in the methionine choline‐deficient (MCD) model of non‐alcoholic fatty liver disease (NAFLD). Methods: Iron‐loaded rats were fed either MCD or control diets [MCD diet plus choline bitartrate (2 g/kg) and dl‐methionine (3 g/kg)] for 4 and 12 weeks, after which liver pathology, hepatic iron, triglyceride, lipid peroxidation products and hydroxyproline (HYP) levels and serum alanine aminotransferase (ALT) levels were evaluated. Results: Iron supplementation in MCD animals resulted in histologic evidence of hepatic iron overload at 4 and 12 weeks and a 14‐fold increase in hepatic iron concentration at 12 weeks (P<0.001). Iron supplementation in these animals was associated with increased lobular necroinflammation at 4 weeks (P<0.02) and decreased hepatic steatosis (P<0.01), hepatic triglyceride levels (P<0.01), hepatic‐conjugated dienes (CD; P<0.02) and serum ALT levels (P<0.002) at 12 weeks. Reduced hepatic steatosis (P<0.005) and CD (P<0.01) were apparent by 4 weeks. Iron supplementation was associated with a trend towards increased perivenular fibrosis not hepatic HYP content. Conclusion: Hepatic iron overload in the MCD model of NAFLD is associated with decreased hepatic lipid, decreased early lipid peroxidation products, increased necroinflammation and a trend towards increased perivenular fibrosis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of iron overload in a rat nutritional model of non‐alcoholic fatty liver disease

Kirsch

Sijtsema²,

Tlali³

et al. 2006

Liver International

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“…HIROSUE and HOSOGAI 1993;EDER et al 1996). The fatty acid data from this experiment, expressed as mo1/100 mol and pmol/g, suggest an impairment in hepatic essential and monoenoic fatty acid metabolism also in the face of moderate and slight iron depletion.…”

Section: Discussionmentioning

confidence: 91%

“…Liver as the main target organ for measuring the fatty acid variation often shows an increase in desaturation substrates at the expense of desaturation products in severely iron-deficient rats (e.g. HIROSUE and HOSOGAI 1993;EDER et al 1996). The fatty acid data from this experiment, expressed as mo1/100 mol and pmol/g, suggest an impairment in hepatic essential and monoenoic fatty acid metabolism also in the face of moderate and slight iron depletion.…”

Section: Discussionmentioning

confidence: 99%

“…Inter a l k , it has been found, that iron is a structural component of the A-9 and A-6 desaturases (STRITTMATTER et al 1974;OKAYASU et al 1981). Accordingly, experiments with animal models have revealed a strong relationship between dietary iron and fatty acid composition of tissues mainly including the liver (RAO et al 1980;JOHNSON et al 1989;HIROSUE and HOSOGAI 1993;EDER et al 1996) and plasma (RAO et al 1980;SHERMAN et al 1982;CUNNANE and MCADOO 1987;JOHNSON et al 1989). Unfortunately, most of these results are conflicting and derived from studies with severe iron deficiency.…”

Section: Introductionmentioning

confidence: 99%

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Moderate iron deficiency and fatty acid shifting in liver and brain of rats

Stangl¹,

Kirchgeßner²

1998

Animal Physiology Nutrition

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Zusammenfassung Moderater Eisenmangel und Fettsäure‐Verschiebungen in Leber und Gehirn von Ratten Eisenmangel ist eine der häufigsten ernährungsbedingten Mangelerscheinungen bei Mensch und Tier. Die vorliegende Studie mit Ratten sollte den Einfluß eines mäßigen und leichten Eisenmangels auf die Zusammensetzung der Fettsäuren in den diätetisch verschieden stark beeinflußbaren Organen Leber und Gehirn untersuchen. Dazu wurden entwöhnte männliche Ratten in 7 Gruppen, zu je 12 Tieren eingeteilt. 3 Gruppen erhielten Diäten mit suboptimalen Eisengehalten von 9, 13 und 18 mg/kg über einen Zeitraum von 5 Wochen. Den Mangelgruppen wurden 3 pair‐fed Kontrollgruppen und eine ad libitum Kontrollgruppe zugeordnet, deren Rationen 50 mg Eisen/kg enthielten. Der moderate Eisenmangel ließ sich innerhalb eines Versuchzeitraumes von 5 Wochen induzieren. Hämoglobinkonzentration, Hämatokrit sowie die Eisenkonzentrationen in Serum und Leber spiegelten den Eisenstatus am deutlichsten wider und zeigten sich nach der 5‐wöchigen Versuchsperiode bei den Eisenmangeltieren signifikant erniedrigt. Die Fettsäurezusammensetzung der Gesamtlipide der Leber konnte durch die alimentäre Eisendepletion deutlich beeinflußt werden und veränderte sich am stärksten bei Tieren, deren Diät 9 mg Eisen/kg enthielt. Die Anteile und Konzentrationen der Leberfettsäuren wurden darüber hinaus auch geringfügig von der Lebendmasse der Tiere beeinflußt. Bei der durch Eisenmangel veränderten Fettsäurezusammensetzung, erwiesen sich verminderte Anteile und Gehalte einzelner Monoenfettsäuren sowie eine Anreicherung der Linolsäure (18:2 n‐6) und α‐Linolensäure (18:3 n‐3) am bedeutendsten. Der Quotient aus 20:4 (n‐6) und 18:2 (n‐6), einem Marker für gestörte Δ‐6 bzw. Δ‐5 Desaturation, zeigte sich in Ratten mit niedrigster Eisenzulage signifikant vermindert. Im Gehirn ließen sich aufgrund des Eisenmangels weniger Fettsäureveränderungen beobachten als in der Leber. Am auffälligsten waren jedoch in allen Gruppen mit Eisenmangel ein erhöhter Anteil an 18:2 (n‐6) und ein verminderter Anteil an 22:4 (n‐6) gegenüber den paid‐fed Kontrollgruppen. Auch der Quotient aus den (n‐6) Fettsäuren 20:4 und 18:2 lag in den Gehirnlipiden der Eisenmangeltiere signifikant niedriger als in den pair‐fed Kontrolltieren. Da Fettsäuren im Körper bedeutende Funktionen als Strukturbestandteile von Zellmembranen oder als Vorstufen von Eicosanoiden aufweisen, sind Eisenmangel‐induzierte Fettsäureverschiebungen in Leber und Gehirn möglicherweise von physiologischen Störungen begleitet.

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Different degrees of moderate iron deficiency modulate lipid metabolism of rats

Stangl

Kirchgeßner

1998

Lipids

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Severe iron deficiency affects lipid metabolism. To investigate whether moderate iron depletion also alters lipid variables-including lipid levels in serum and liver, hepatic lipogenesis, and fatty acid composition indicative of an impaired desaturation-we carried out experiments with rats fed 9, 13, and 18 mg iron/kg diet over a total of 5 wk. The study also included three pair-fed control groups and an ad libitum control group, fed with 50 mg iron/kg diet. The iron-depleted rats were classified as iron-deficient on the basis of reduced serum iron, hemoglobin concentration, and hematocrit. All moderately iron-deficient rats had significantly lower cholesterol concentrations in liver and serum lipoproteins than their pair-fed controls. Rats with the lowest dietary iron supply had higher concentrations of hepatic phosphatidylcholine (PC) and phosphatidylethanolamine (PE), lower activities of glucose-6-phosphate dehydrogenase, malic enzyme and fatty acid synthase, and higher triacylglycerol concentrations in serum lipoproteins than the corresponding pair-fed control rats. Moderate iron deficiency also depressed the serum phospholipid level. Moreover, several consistent significant differences in fatty acid composition of hepatic PC and PE occurred within moderate iron deficiency, which indicate impaired desaturation by delta-9 and delta-6 desaturases of saturated and essential fatty acids. We conclude that lipid variables, including cholesterol in liver and serum lipoproteins as well as fatty acid desaturation, reflect the gradations of iron status best and can be used as an indicator of the degree of moderate iron deficiency.

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Changes of hepatic lipid and fatty acid profiles in rats administered iron-deficient diet and ethanol

Cited by 7 publications

References 16 publications

Effects of iron overload in a rat nutritional model of non‐alcoholic fatty liver disease

Effects of iron overload in a rat nutritional model of non‐alcoholic fatty liver disease

Moderate iron deficiency and fatty acid shifting in liver and brain of rats

Different degrees of moderate iron deficiency modulate lipid metabolism of rats

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