Changes of glycosaminoglycan composition of uterine myometrium of rabbit induced by female sex steroids.

Abstract

Glycosaminoglycan fractions were separately prepared from the myometrium of control, estrogen-treated, and estrogen-progesterone-treated rabbits after digestion with pronase. The glycosaminoglycan composition of each glycosaminoglycan fraction was determined by digestion with specific mucopolysaccharidases and nitrous acid. It was found that foregoing hormones showed no effects on the total glycosaminoglycan content, expressed as mg per g of the delipidated dry tissue. These hormones, however, markedly affected the glycosaminoglycan composition. As compared with the control, the estrogen-treatment decreased the contents of hyaluronic acid and dermatan sulfate, but increased those of chondroitin 4- and 6-sulfates. The effect of estrogen on the content of hyaluronic acid was strikingly enhanced by subsequent treatment with progesterone, and those of chondroitin 4- and 6-sulfates were also enhanced. On the other hand, progesterone abolished effect on the content of dermatan sulfate. Although no effect was observed on the content of heparan sulfate by the estrogen treatment, subsequent treatment with progesterone decreased the content of this glycosaminoglycan.

Histologic, morphometric, and immunocytochemical analysis of myometrial development in rats and mice: I. Normal development

“…It is unclear if metabolism of ECM is regulated hormonally in neonatal rodents because the neonatal ovary is endocrinologically inactive (Csernus, 1986) and because maternal estrogen present at birth is probably bound to alpha-fetoprotein (Henry and Miller, 1986;Raynaud, 1973). In mature uteri, ECM proteins are regulated directly or indirectly by ovarian hormones (Salvador and Tsai, 1973;Woessner, 1969;Jeffrey and Koob, 1973;Jeffrey et al, 1975;Komm et al, 1987;Kao et al, 1964Kao et al, ,1969Dyer et al, 1980;Mandell and Sodek, 1982;Munakata et al, 1984Munakata et al, ,1985Mochizuki and Tojo, 1978;Ozasa et al, 1981;Sakyo et al, 1986). In this regard estrogens have been shown to increase collagen content in the uterus (Komm et al, 1987;Kao et al, 1964Kao et al, , 1969Dyer et al, 1980), and progesterone regulates uterine collagenase activity IGreas, 1976;Jeffrey et al, 1975).…”

Histologic, morphometric, and immunocytochemical analysis of myometrial development in rats and mice: II. Effects of DES on development

“…Mandell and Sodek, 1982;Munakata et al, 1984Munakata et al, ,1985Mochizuki and Tojo, 1978;Ozasa et al, 1981;Sakyo et al, 1986). Estrogens increase collagen content in the uterus (Komm et al, 1987;Kao et al, 1964Kao et al, ,1969Dyer et al, 1980), and progesterone regulates uterine collagenase activity and thus uterine collagen content (Gross, 1976;Jeffrey et al, 1975).…”