Changes of DNA topology affect the global transcription landscape and allow rapid growth of a Bacillus subtilis mutant lacking carbon catabolite repression

Reuß, Daniel R.; Rath, Hermann; Thürmer, Andrea; Benda, Martin; Daniel, Rolf; Völker, Uwe; Mäder, Ulrike; Commichau, Fabian M.; Stülke, Jörg

doi:10.1016/j.ymben.2017.12.004

Cited by 17 publications

(13 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Contradictory results have been reported about the essentiality of the topA gene encoding topo I in B. subtilis . While topA was found to be essential in two studies (10,11), the gene could be deleted in other genome-wide analyses (8,35). However, Koo et al.…”

Section: Resultsmentioning

confidence: 97%

“…Both strains carried a point mutation in the mreB gene, and an insertion of an A in the terminator region of the yfhJ gene. Mutations in mreB are frequently encountered in suppressor screens (11), and the mutation in the yfhJ terminator is not expected to have a phenotypic effect. Therefore, these mutations can be regarded as genetic hitchhikers.…”

Section: Resultsmentioning

confidence: 99%

“…This observation suggests that the bacteria rapidly exhaust a particular nutrient from the medium, and that they experience difficulties in swiftly adapting their metabolism. In this context, it is interesting to note that we have observed large scale alterations in central metabolism in B. subtilis as a result of changes in DNA topology (11). Strikingly, glutamate biosynthesis was more efficient as a result of increased topo I activity which in turn may suggest that the expression of the gltAB operon encoding glutamate synthase is reduced upon loss of topo I. Interestingly, we found point mutations in two genes involved in glutamate metabolism in the originally isolated suppressor strain that had lost the amplification of the parEC region.…”

Section: Discussionmentioning

confidence: 98%

“…Some laboratory strains of B. subtilis (JH642 and its derivatives) even lack a chromosomal region of 18 kb that encompasses the topB gene (9). For topo I encoded by the topA gene, contradicting results have been reported: The gene is essential in E. coli , and two studies reported it to be essential in B. subtilis (7,10,11). In contrast, in a recent genome-wide essentiality study, the topA gene could be deleted; however only by replacing the gene with a kanamycin cassette whereas replacements with other resistance cassettes were not successful (8).…”

Section: Introductionmentioning

confidence: 92%

See 3 more Smart Citations

Topoisomerase IV can functionally replace all type 1A topoisomerases in Bacillus subtilis

Reuß

Faßhauer

Mroch

et al. 2019

Nucleic Acids Research

Self Cite

View full text Add to dashboard Cite

DNA topoisomerases play essential roles in chromosome organization and replication. Most bacteria possess multiple topoisomerases which have specialized functions in the control of DNA supercoiling or in DNA catenation/decatenation during recombination and chromosome segregation. DNA topoisomerase I is required for the relaxation of negatively supercoiled DNA behind the transcribing RNA polymerase. Conflicting results have been reported on the essentiality of the topA gene encoding topoisomerase I in the model bacterium Bacillus subtilis . In this work, we have studied the requirement for topoisomerase I in B. subtilis . All stable topA mutants carried different chromosomal amplifications of the genomic region encompassing the parEC operon encoding topoisomerase IV. Using a fluorescent amplification reporter system we observed that each individual topA mutant had acquired such an amplification. Eventually, the amplifications were replaced by a point mutation in the parEC promoter region which resulted in a fivefold increase of parEC expression. In this strain both type I topoisomerases, encoded by topA and topB , were dispensable. Our results demonstrate that topoisomerase IV at increased expression is necessary and sufficient to take over the function of type 1A topoisomerases.

show abstract

Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 92%

See 2 more Smart Citations

Topoisomerase IV can functionally replace all type 1A topoisomerases in Bacillus subtilis

Reuß

Faßhauer

Mroch

et al. 2019

Nucleic Acids Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…With seryl-phosphorylation Hpr (HPr-Ser-P) acting as its cofactor, CcpA can bind with the catabolite-responsive element (CRE) box to regulate numerous catabolic genes . Alleviating the CCR effect can enhance the utilization of nondominant carbon sources. − …”

Section: Introductionmentioning

confidence: 99%

Boosting the Heterologous Expression of d-Allulose 3-Epimerase in Bacillus subtilis through Protein Engineering and Catabolite-Responsive Element Box Engineering

Liu

Wang

Liu

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

As a natural sweetener with low calories and various physiological activities, d-allulose has drawn worldwide attention. Currently, d-allulose 3-epimerase (DAEase) is mainly used to catalyze the epimerization of d-fructose to d-allulose. Therefore, it is quite necessary to enhance the food-grade expression of DAEase to meet the surging market demand for d-allulose. In this study, initially, the promising variant H207L/D281G/C289R of Clostridium cellulolyticum H10 DAEase (CcDAEase) was generated by protein engineering, the specific activity and the T 1/2 of which were 2.24-fold and 13.45-fold those of the CcDAEase wild type at 60 °C, respectively. After that, PamyE was determined as the optimal promoter for the recombinant expression of CcDAEase in Bacillus subtilis, and catabolite-responsive element (CRE) box engineering was further performed to eliminate the carbon catabolite repression (CCR) effect. Lastly, high-density fermentation was carried out and the final activity peaked at 4971.5 U mL–1, which is the highest expression level and could effectively promote the industrial production of DAEase. This research provides a theoretical basis and technical support for the molecular modification of DAEase and its efficient fermentation preparation.

show abstract

Towards next-generation model microorganism chassis for biomanufacturing

Liu

et al. 2020

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Changes of DNA topology affect the global transcription landscape and allow rapid growth of a Bacillus subtilis mutant lacking carbon catabolite repression

Cited by 17 publications

References 52 publications

Topoisomerase IV can functionally replace all type 1A topoisomerases in Bacillus subtilis

Topoisomerase IV can functionally replace all type 1A topoisomerases in Bacillus subtilis

Boosting the Heterologous Expression of d-Allulose 3-Epimerase in Bacillus subtilis through Protein Engineering and Catabolite-Responsive Element Box Engineering

Towards next-generation model microorganism chassis for biomanufacturing

Contact Info

Product

Resources

About