Changes of DNA content per nucleus in hepatocytes of rat during the first days of postnatal life

C, Bibbiani; Tongiani, Roberto; Viola-Magni, Maria Pia

doi:10.1007/bf00305931

Cited by 9 publications

(4 citation statements)

References 21 publications

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“…Summing up, the results of the present work show that: (1) the synthesis of DNA occurring in the hepatocytes during the first day of life is not a premitotic one, but is related to the increase of the amount of DNA per nucleus which occurs after birth (Bibbiani et al, 1972); (ii) decrease of 26-35:;, of label takes place during 4 h (from 4 to 8 a.m.) of the second day of life, probably as a consequence of DNA turnover.…”

Section: Discussionsupporting

confidence: 53%

“…This difference between the diploid kidney tubule nuclei and diploid hepatic nuclei of newborn rats can be explained on the basis of previous findings, which show that the DNA content of newborn diploid hepatocytes is less than that of adult diploid ones (Bibbiani et al, 1972).…”

Section: ) Counts Of Mitosesmentioning

confidence: 61%

“…This fact provides conclusive evidence that the loss of label, taking place in the hepatic nuclei from 4 to 8 a.m. of the first day after birth is not due to mitotic division. Therefore, the synthesis of DNA, which takes place in IS:,, of the nuclei 3 h after birth, cannot be regarded as premitotic, but is likely to be related to the increase of the amount of DNA per diploid nucleus, which occurs during the first 3 days of life (Bibbiani et al, 1972).…”

Section: Discussionmentioning

confidence: 99%

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Synthesis and turnover of DNA in hepatocytes of neonatal rats

Mp¹

1972

Journal of Microscopy

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The incorporation of 3H-thymidine has been studied in the hepatocytes nuclei of rats during the first 3 days of life. The synthesis of DNA which occurs in lWO of nuclei after a single injection of the labelled precursors 2 h after birth, cannot be considered as premitotic since:(i) The hepatic cell number is constant during the first 3 days of life.(ii) The mitotic index is less than O.l(:,, 20 times lower than that expected on the basis of the labelling index and of the length of cellular cycle.(iii) The percentage of labelled nuclei does not increase in the following 3 days.(iv) the average number of grains per nucleus is unchanged for 18 h after the injection, a period longer than an entire cellular cycle (13 h).This synthesis is better explained as due to an increase of DNA content per nucleus, previously described, in the hepatocytes during the first 3 days of life.After 20 h of life (18 h after label injection) a decrease of label of about 30",, takes place without variation of DNA-Feulgen dye content per nucleus. It is concluded that synthesis and turnover of DNA are present in the hepatocytes nuclei and that these processes are probably related to the functional maturation of the cells.

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Section: Discussionsupporting

confidence: 53%

Section: ) Counts Of Mitosesmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 99%

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Synthesis and turnover of DNA in hepatocytes of neonatal rats

Mp¹

1972

Journal of Microscopy

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“…However, it is possible that there was a real increase in the DNA content of the adipocytes. The DNA content of rat hepatocyte nuclei, measured by several different methods including Feulgen photometry and microinterferometry, has been shown to increase from 5.1 pg/nucleus in 20-day-old fetuses to 8.1 pg/nucleus in 6-dayold rats (2).…”

Section: Discussionmentioning

confidence: 99%

Development of Perirenal Adipose Tissue in the Neonatal Lamb: Effects of Dietary Safflower Oil

Vernon¹

1977

Neonatology

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Perirenal adipose tissue development was investigated in lambs from birth until 17 days of age. Changes in the morphology, the lipid, protein, RNA and DNA content, the adipocyte number and size, and the rate of protein, RNA and DNA synthesis of the perirenal fat-pads are reported. During the first 9 days after birth the tissue lost the morphological characteristics of brown adipose tissue and acquired those of white adipose tissue. As there was no change in either the number of adipocytes or the DNA content of the fat-pads between birth and 9 days of age it is concluded that there was a differentiation of brown into white adipocytes. Over 80% of the increase in tissue weight between birth and 17 days of age was due to lipid deposition. Administration of safflower oil to the lambs had no apparent effect on the change from brown to white fat, but it markedly reduced the rate of lipid deposition between days 9 and 17 post partum.

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Chromatin phospholipid changes during rat liver development

Albi

Magni

Lazzarini

et al. 1991

Cell Biochemistry & Function

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The chromatin extracted from rat hepatocytes of different ages has been shown to contain a phospholipid fraction representing 0.47-0.59 per cent of total chromatin in newborn animals and 0.22 per cent in 45-day-old animals. No such age-related differences are observed in the nuclei. The phospholipid composition of the nuclei at different ages shows a higher level of sphingomyelin and a lower level of phosphatidylserine in newborn than in adult animals. Chromatin phospholipids have a completely different composition from that of nuclei with respect to age, particularly in newborn rats, where there is a decrease in phosphatidylcholine and an increase in phosphatidylserine.

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Changes of DNA content per nucleus in hepatocytes of rat during the first days of postnatal life

Cited by 9 publications

References 21 publications

Synthesis and turnover of DNA in hepatocytes of neonatal rats

Synthesis and turnover of DNA in hepatocytes of neonatal rats

Development of Perirenal Adipose Tissue in the Neonatal Lamb: Effects of Dietary Safflower Oil

Chromatin phospholipid changes during rat liver development

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