Changes of defense proteins in the extracellular proteome of grapevine (Vitis vinifera cv. Gamay) cell cultures in response to elicitors

A reliable protocol for the stable transformation of non-embryogenic cells cultures of grapevine (Vitis vinifera L.) and Taxus x media

“…Elicitation was carried out as described in Bru et al [21] in 250 ml Erlenmeyer flasks that contained a final volume of 100 ml of culture. Elicitor concentrations were 50 mM for MBCD and 100 μM for MeJA, according to previous experiments carried out in a dose-response study [23]. After 72 h, medium cultures were collected by filtration with slight vacuum.…”

“…Two major strategies can be carried out to overcome this drawback: elicitation and metabolic engineering. Elicitation is an empirical approach done to stimulate cell cultures to produce secondary metabolites; for instance, grapevine cell cultures elicited with methylated cyclodextrins (MBCD) alone [21] or combined with methyl jasmonate (MeJA) [22,23] has proven an efficient system for the bioproduction of t-R, with 30% overall conversion of feeding sucrose carbon into resveratrol carbon [24]. Metabolic engineering has been currently applied to microorganisms as an alternative way for the bioproduction of t-R and analogues [25][26][27][28] due to the obvious advantages over plant cell cultures to develop transformed strains and to upscale the productive process.…”

Enhancement of phytosterols, taraxasterol and induction of extracellular pathogenesis-related proteins in cell cultures of Solanum lycopersicum cv Micro-Tom elicited with cyclodextrins and methyl jasmonate

“…Therefore, SCC under elicitation constitute a useful model system of reduced complexity to investigate these defense responses since the spent medium of a SCC can be regarded as a continuous cell wall that provides a convenient, continuous and unique source of cell wall proteins, and where all the occur particular aspects of cell wall metabolism occur, including those involved in defense. Likewise, the advantage of using elicited SCC is that the culture medium can be easily separated from cells without cell disruption to recover the free extracellular proteins and metabolites (Martínez-Esteso et al, 2009). Another sequential event in elicitor-induced defense responses is the production of jasmonates that coordinate the activation of a large set of defense responses, and when applied exogenously, they are able to induce resistance in plants.…”

Effect of UV Light on Secondary Metabolite Biosynthesis in Plant Cell Cultures Elicited with Cyclodextrins and Methyl Jasmonate

“…The most frequently used elicitors are of fungal and yeast origin, jasmonic acid and MJ, chitosan, metal ions and UV light. More recently, special attention has been paid to the use of CDs as true elicitors, which act inducing defence responses, which include pathogenesis-related proteins and phytoalexin synthesis, especially the stilbenes like trans-resveratrol in Vitis sp (Morales et al, 1998;Bru et al, 2006;Zamboni et al, 2009;Martinez-Esteso et al 2009), the accumulation of sesquiterpenes and phytosterols as well as pathogenesis-related proteins in Capsicum sp (Sabater-Jara et al, 2010b;Sabater-Jara et al, 2011), the increase of ajmalicine and catharanthine in C. roseus and the enhancement of the production of silymarin in Silybum marianum (L.) Gaernt cell cultures . The success in the production of secondary metabolites with the use of CDs lies in the properties of these compounds since they act not only as elicitors but also forming inclusion complexes (clathrates) with stilbenes (Morales et al, 1998), sesquiterpenes (Sabater-Jara et al, 2010b), indole alkaloids and silymarins , favouring the substantial accumulation of these metabolites and also preventing the toxic and/or inhibitory effect of their extracellular accumulation on cells cultured in suspension.…”