Changes of chemical chronic toxicity to Daphnia magna under different food regimes

Pavlaki, Maria D.; Ferreira, Abel L.G.; Soares, Amadeu M.V.M.; Loureiro, Susana

doi:10.1016/j.ecoenv.2014.07.039

Cited by 17 publications

(10 citation statements)

References 64 publications

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“…The use of different culture conditions across participating labs are likely to explain observed differences in sensitivities and reproductive outputs. In line with the previous argument, several studies have reported that the use of different algal diets, food ratios, water types and culture volumes may affect reproduction in Daphnia and its life cycle performance across pollutants (Barry et al, 1995; Ginjupalli et al, 2015; Hansen et al, 2008; Heugens et al, 2006; Martínez-Jerónimo et al, 2000; Pavlaki et al, 2014; Samel et al, 1999). About 8% of the variation was not explained by any of the studied factors and thus it was associated to intra-lab variability.…”

Section: Discussionsupporting

confidence: 64%

Validation of a two-generational reproduction test in Daphnia magna: An interlaboratory exercise

Barata

Campos

Rivetti

et al. 2017

Science of The Total Environment

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Effects observed within one generation disregard potential detrimental effects that may appear across generations. Previously we have developed a two generation Daphnia magna reproduction test using the OECD TG 211 protocol with a few amendments, including initiating the second generation with third brood neonates produced from first generation individuals. Here we showed the results of an inter-laboratory calibration exercise among 12 partners that aimed to test the robustness and consistency of a two generation Daphnia magna reproduction test. Pyperonyl butoxide (PBO) was used as a test compound. Following experiments, PBO residues were determined by TQD-LC/MS/MS. Chemical analysis denoted minor deviations of measured PBO concentrations in freshly prepared and old test solutions and between real and nominal concentrations in all labs. Other test conditions (water, food, D. magna clone, type of test vessel) varied across partners as allowed in the OECD test guidelines. Cumulative fecundity and intrinsic population growth rates (r) were used to estimate “No observed effect concentrations “NOEC using the solvent control as the control treatment. EC10 and EC-50 values were obtained regression analyses. Eleven of the twelve labs succeeded in meeting the OECD criteria of producing >60 offspring per female in control treatments during 21 days in each of the two consecutive generations. Analysis of variance partitioning of cumulative fecundity indicated a relatively good performance of most labs with most of the variance accounted for by PBO (56.4%) and PBO by interlaboratory interactions (20.2%), with multigenerational effects within and across PBO concentrations explaining about 6% of the variance. EC50 values for reproduction and population growth rates were on average 16.6 and 20.8% lower among second generation individuals, respectively. In summary these results suggest that the proposed assay is reproducible but cumulative toxicity in the second generation cannot reliably be detected with this assay.

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Section: Discussionsupporting

confidence: 64%

Validation of a two-generational reproduction test in Daphnia magna: An interlaboratory exercise

Barata

Campos

Rivetti

et al. 2017

Science of The Total Environment

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“…Therefore, unlike the study looking at the uptake of different sized polystyrene, there is no evidence that MPs are eaten in proportion to their concentration when algae are present. This implies selectivity ( Pavlaki et al, 2014 ). Previous studies have demonstrated that Daphnia are not blindly filtering particles from the water, since particles smaller than the mesh size of their appendages are still eaten ( Pavlaki et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

“…When exposed to a single concentration of MPs Daphnia almost immediately ate them in large quantities. Previous studies have demonstrated that Daphnia will feed on polystyrene, beating their appendages at a constant rate regardless of the food concentration ( Pavlaki et al, 2014 ). The same study also found that plastics of different sizes (1.1 µm and 5.7 µm) were ingested in proportion to their concentration ( Pavlaki et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have demonstrated that Daphnia will feed on polystyrene, beating their appendages at a constant rate regardless of the food concentration ( Pavlaki et al, 2014 ). The same study also found that plastics of different sizes (1.1 µm and 5.7 µm) were ingested in proportion to their concentration ( Pavlaki et al, 2014 ). This could explain the fact that the amount eaten was reduced when food (algae) was present, which suggests a simple competition for uptake.…”

Section: Discussionmentioning

confidence: 99%

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Impact of polystyrene microplastics on Daphnia magna mortality and reproduction in relation to food availability

Al-Jaibachi

Callaghan

2018

PeerJ

126

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Microplastics (MPs) in the environment continue to be a growing area of concern in terms of acute and chronic impacts on aquatic life. Whilst increasing numbers of studies are providing important insights into microparticle behaviour and impacts in the marine environment, a paucity of information exists regarding the freshwater environment. This study focusses on the uptake, retention and the impact of 2 µm polystyrene MPs in the freshwater cladoceran Daphnia magna in relation to food intake (algae Chlorella vulgaris), with MP size chosen to approximately match the cell size of the algae. Daphnia were exposed to varied concentrations of MPs and algae. When exposed to a single concentration of MPs Daphnia almost immediately ate them in large quantities. However, the presence of algae, even at low concentrations, had a significant negative impact on MP uptake that was not in proportion to relative availability. As MP concentrations increased, intake did not if algae were present, even at higher concentrations of MPs. This suggests that Daphnia are selectively avoiding eating plastics. Adult Daphnia exposed to MPs for 21 days showed mortality after seven days of exposure in all treatments compared to the control. However significant differences were all related to algal concentration rather than to MP concentration. This suggests that where ample food is present, MPs have little effect on adults. There was also no impact on their reproduction. The neonate toxicity test confirmed previous results that mortality and reproduction was linked to availability of food rather than MP concentrations. This would make sense in light of our suggestion that Daphnia are selectively avoiding eating microplastics.

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“…D. magna is a suitable model for understanding life history trade-offs of dietary restriction as there is already a vast amount of literature available on relationship between food concentrations and daphnid life history strategies (Latta IV et al, 2011). There is also an increasing interest in understanding how diet plays a role in determining the toxicant sensitivity of different organisms (Pavlaki et al, 2014). For example, algal diet and lipid concentrations have been proposed to be confounding variables in pesticide-induced male production (Olmstead and LeBlanc, 2003; Ginjupalli et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Perturbations in polar lipids, starvation survival and reproduction following exposure to unsaturated fatty acids or environmental toxicants in Daphnia magna

2016

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Acclimating to toxicant stress is energy expensive. In laboratory toxicology tests dietary conditions are ideal, but not in natural environments where nutrient resources vary in quality and quantity. We compared the effects of additional lipid resources, docosahexaenoic acid (n-3; DHA) or linoleic acid (n-6; LA), or the effects of the toxicants, atrazine or triclosan on post-treatment starvation survival, reproduction, and lipid profiles. Chemical exposure prior to starvation had chemical-specific effects as DHA showed moderately beneficial effects on starvation survival and all of the other chemicals showed adverse effects on either survival or reproduction. Surprisingly, pre-exposure to triclosan inhibits adult maturation and in turn completely blocks reproduction during the starvation phase. The two HR96 activators tested, atrazine and LA adversely reduce post-reproduction survival 70% during starvation and in turn show poor fecundity. DHA and LA show distinctly different profiles as DHA primarily increases the percentage of large (>37 carbon) phosphatidylcholine (PC) species and LA primarily increases the percentage of smaller (<37 carbon) PC species. The toxicants atrazine and triclosan moderately perturb a large number of different phospholipids including several phosphatidylethanolamine species. Some of these polar lipid species may be biomarkers for diets rich in specific fatty acids or toxicant classes. Overall our data demonstrates that toxicants can perturb lipid utilization and storage in daphnids in a chemical specific manner, and different chemicals can produce distinct polar lipid profiles. In summary, biological effects caused by fatty acids and toxicants are associated with changes in the production and use of lipids.

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Changes of chemical chronic toxicity to Daphnia magna under different food regimes

Cited by 17 publications

References 64 publications

Validation of a two-generational reproduction test in Daphnia magna: An interlaboratory exercise

Validation of a two-generational reproduction test in Daphnia magna: An interlaboratory exercise

Impact of polystyrene microplastics on Daphnia magna mortality and reproduction in relation to food availability

Perturbations in polar lipids, starvation survival and reproduction following exposure to unsaturated fatty acids or environmental toxicants in Daphnia magna

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