Changes of adipocytokine expression after diabetic rats received sitagliptin and the molecular mechanism

Wu, Qiaomin; Ni, Haixiang; Lu, Xinli

doi:10.1016/j.apjtm.2016.07.014

Cited by 12 publications

(6 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another significantly enriched pathway was the adipocytokine signaling pathway. Adipocytokines are a type of cytokines synthesized and secreted by adipocytes, and they have a regulatory effect on inflammation, insulin sensitivity, and endothelial function [ 70 , 71 ]. Three pathways, including the adipocytokine signaling pathway, were found to be significantly enriched (P < 0.05) for the targets of novel-mir-14 in the research of breast muscle in the Pekin duck [ 72 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profile analysis of leg muscle tissues between slow- and fast-growing chickens

Dai

Chen

et al. 2018

PLoS ONE

View full text Add to dashboard Cite

Chicken is widely favored by consumers because of some unique features. The leg muscles occupy an important position in the market. However, the specific mechanism for regulating muscle growth speed is not clear. In this experiment, we used Jinghai yellow chickens with different body weights at 300 days as research subjects. The chickens were divided into fast- and slow-growing groups, and we collected leg muscles after slaughtering for use in RNA-seq. After comparing the two groups, 87 differentially expressed genes (DEGs) were identified (fold change ≥ 2 and FDR < 0.05). The fast-growing group had 42 up-regulated genes and 45 down-regulated genes among these DEGs compared to the slow-growing group. Six items were significantly enriched in the biological process: embryo development ending in birth or egg hatching, chordate embryonic development, embryonic skeletal system development, and embryo development as well as responses to ketones and the sulfur compound biosynthetic process. Two significantly enriched pathways were found in the KEGG pathway analysis (P-value < 0.05): the insulin signaling pathway and the adipocytokine signaling pathway. This study provides a theoretical basis for the molecular mechanism of chicken growth and for improving the production of Jinghai yellow chicken.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptome profile analysis of leg muscle tissues between slow- and fast-growing chickens

Dai

Chen

et al. 2018

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…From those mentioned mechanisms of SIRT1 and PTEN, the current study exhibited decreased of Akt‐p concentration in hepatic cells of HFD rats. Moreover adiponectin has a vital role in the insulin resistance pathway; it has a regulating influence on insulin sensitivity (Wu, Ni, & Lu, ). It was noticed that disturbance in lipid metabolism and excessive fat deposition leads to abnormal synthesis of adiponectin (Burnstock & Gentile, ).…”

Section: Discussionmentioning

confidence: 99%

Berberine chloride ameliorated PI3K/Akt‐p/SIRT‐1/PTEN signaling pathway in insulin resistance syndrome induced in rats

et al. 2019

View full text Add to dashboard Cite

The liver is the main organ involved in lipid metabolism process and it helps in drug detoxification. Insulin resistance is considered one of risk reasons which lead to several metabolic diseases. Currently, berberine (BER) occupies a huge challenge against multiple diseases with no toxic effect. The present work was aimed to identify, does BER-chloride has a poisonous influence on the liver? and investigating the outcome of BER-chloride on PI3K/Akt-p/SIRT-1/PTEN pathway during insulin resistance syndrome. The insulin resistance model was achieved in experimental female rats via high-fat diet (HFD). Glucose, insulin, lipid profiles, and hepatic oxidative stress parameters were assessed. PI3K, AKt-p, SIRT-1, and PTEN levels in hepatic tissue were determined at genome and protein levels. Further adiponectin concentration was performed in serum, hepatic, and white adipose tissues. Molecular study of fold alteration in insulin, insulin receptor, and retinol binding protein-4 (RBP4) in liver was done. Practical applicationsObesity syndrome causes multiple obstacles in modern years. The current results revealed elevation the body weight of rats, plasma glucose, homeostatic model assessment, glycated hemoglobin, insulin, and lipid profiles concentrations in a group of rats, which nourished HFD for 8 weeks and this rise, was diminished after 2 weeks from BER-chloride administration. Further, BER-chloride improved transaminases enzymes, pro-oxidant, and antioxidant defense system, PI3K, AKt-p, SIRT-1, and PTEN in the liver, with downregulation of hepatic RBP4. Hence, these data provide a crucial message that BER-chloride enhanced both hepatic function and insulin signaling pathways that might be of therapeutic importance to insulin resistance with no harmful effect on the liver. BER-chloride is predicted to be a drug of choice for obesity complications cure. K E Y W O R D Sberberine chloride, hepatotoxicity, insulin receptor, liver

show abstract

“…Moreover adiponectin has an important role in insulin resistance pathway; it is a member of adipocytokines which secreted by adipocytes and has a regulating effect on insulin sensitivity [40]. It was reported that disturbance in lipid metabolism and excessive fat deposition leads to abnormal synthesis of adipocytokines [41].…”

Section: Discussionmentioning

confidence: 99%

Berberine chloride ameliorated PI3K/Akt-p/SIRT-1/PTEN signaling pathway in insulin resistance syndrome-induced rats

El‐Zeftawy

Ghareeb

2018

Preprint

View full text Add to dashboard Cite

19Insulin resistance is one of dangerous factors as it leads to numerous metabolic 20 disorders such as non-insulin dependent diabetes mellitus. It affects most tissues 21 mainly adipose tissue, liver and muscle. Nowadays, berberine has several medical 22 applications against diseases. The current study was carried out to identify the effect 23 of berberine chloride (BER-chloride) on phosphatidyl inositol-3-kinase/ 24 phosphorylated protein kinase B/ sirtuin type 1/ phosphatase and tension homologue 25 (PI3K/Akt-p/SIRT-1/PTEN) pathway during insulin resistance phenomena. Insulin 26 resistance model was performed in experimental rats by using high fat diet. Plasma 27 glucose, serum insulin, lipid profiles, hepatic oxidative stress markers were estimated. 28 Serum transaminases activities and kidney function tests were determined. Further, 29 hepatic PI3K, AKt-p, SIRT-1; PTEN levels were assayed. The concentration of 30 adiponectin in serum, hepatic tissue and white adipose tissue was determined. 31 Moreover, fold change in hepatic insulin, insulin receptor and retinol binding protein-32 4 (RBP4) at molecular level was performed. Histopathological study of white adipose 33 tissue was also determined. The results showed increase the rats' body weights, blood 34 glucose, homeostatic model assessment, glycated hemoglobin, insulin and lipid 35 profiles levels in group of rats fed on high fat diet for eight weeks and this elevation 36 was decreased after administration of BER-chloride for two weeks. Further, BER-37 chloride administration exhibited improvement of oxidative stress parameters, PI3K, 38 AKt-p, SIRT-1 and PTEN. This was associated with down-regulation of RBP4.39According to these data we conclude that, BER-chloride mediated several insulin 40 signaling pathways that could be of therapeutic significance to insulin resistance. 41

show abstract

Changes of adipocytokine expression after diabetic rats received sitagliptin and the molecular mechanism

Abstract: Sitagliptin can regulate the expression of adipocytokines in adipose tissue of diabetic rats through PI3K-AKT pathway.

Cited by 12 publications

References 20 publications

Transcriptome profile analysis of leg muscle tissues between slow- and fast-growing chickens

Transcriptome profile analysis of leg muscle tissues between slow- and fast-growing chickens

Berberine chloride ameliorated PI3K/Akt‐p/SIRT‐1/PTEN signaling pathway in insulin resistance syndrome induced in rats

Berberine chloride ameliorated PI3K/Akt-p/SIRT-1/PTEN signaling pathway in insulin resistance syndrome-induced rats

Contact Info

Product

Resources

About