Changes inβ-actin mRNA Expression in Remodeling Canine Myocardium

Carlyle, Wenda C.; Toher, Cynthia; Vandervelde, Jonathan R.; McDonald, Kenneth; Homans, D. C.; Cohn, Jay N.

doi:10.1006/jmcc.1996.0006

Cited by 32 publications

(12 citation statements)

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“…We also showed that 3-actin mRNA was highly expressed in PDLE, coinciding with the high expression of S100A4 mRNA. The variation of I-actin mRNA expression observed in our results is supported by other reports in which the expression level of 1-actin mRNA is inconstant and depends on the stage of cell differentiation, stage of tissue development, and origins of the tissues (Spanakis, 1993;Biragyn et al, 1994;Carlyle et al, 1996;Nagahara and Matsuda, 1996). A relatively high expression of S100A4 mRNA was also seen in the pulp tissue (PT).…”

Section: Discussionsupporting

confidence: 91%

cDNA Cloning of S100 Calcium-binding Proteins from Bovine Periodontal Ligament and Their Expression in Oral Tissues

et al. 1998

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The periodontal ligament (PDL) is a unique tissue that is crucial for tooth function. However, little is known of the molecular mechanisms controlling PDL function. To characterize PDL cells at the molecular level, we constructed a cDNA library from bovine PDL tissue. We then focused on the isolation of S100 calcium-binding proteins (CaBPs), because they mediate Ca2+ signaling and control important cellular processes such as differentiation and metabolism. We screened the PDL cDNA library with a mouse S100A4 cDNA, and cloned the bovine cDNAs of two S100 CaBPs (S100A4 and S100A2). In northern blotting analysis, the highest expression of S100A4 was detected in PDL from erupted teeth (PDLE). PDL from teeth under eruption (PDLU) showed a lower expression of S100A4, and its expression in gingiva was faintly detectable. S100A4 expression was also high in the pulp tissue followed by the dental papilla of the tooth germ. S100A2 expression was high in PDLE and gingiva. Interestingly, only PDLE exhibited a high expression of both S100A4 and S100A2. PDLE also expressed the highest level of beta-actin, a target cytoskeletal protein for S100A4. It is conceivable that the high expression of S100A4 in PDLE is a result of the maturation of the PDL and/or a response to mechanical stress generated by mastication. Since there was a marked difference of S100A4 expression between PDL and gingiva, we propose that S100A4 could be a useful marker for distinguishing cells from these two tissues.

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Section: Discussionsupporting

confidence: 91%

cDNA Cloning of S100 Calcium-binding Proteins from Bovine Periodontal Ligament and Their Expression in Oral Tissues

et al. 1998

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“…-actin is a globular protein found in all eukaryotic cells, which is always used as an internal control for gene express [25]. cDNA is a complementary DNA of mRNA from reverse transcriptional mRNA template, which can reflect the quantitative level of mRNA.…”

Section: Resultsmentioning

confidence: 99%

MDR1-targeted siRNA delivery with cationic dendritic conjugated polymers

et al. 2013

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A cationic dendritic polyfluorene (PFP) is examined as a siRNA delivery vector. This material was designed to facilitate the nucleic acid binding, encapsulation and efficient cellular uptake. PFP can effectively protect siRNA against nuclease degradation, which is necessary for gene carriers. PFP can be used for multidrug resistance gene-targeted siRNA delivery in doxorubicin (Dox)-resistant human breast cancer cells (MCF7) cells. As a siRNA transfection agent, PFP can efficiently achieve the reversal of drug resistance and enhance the drug sensitivity. These new features and capabilities represent a major step toward conjugated polymers that can function for therapeutic application.

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“…In vascular SMC, β-actin rather than alpha-actin expression becomes dominant, particularly when SMC undergoes phenotypic modulation [17]. During canine myocardium vascular remodeling, β-actin mRNA is upregulated in transmyocardial by direct-current shock treatment [18]. Therefore, β-actin gene expression has served as a key functional marker for phenotypic modulation of SMC and vascular remodeling.…”

Section: Resultsmentioning

confidence: 99%

Dynamics of smooth muscle cell deadhesion from thermosensitive hydroxybutyl chitosan

Chen¹,

Dang²,

Tan³

et al. 2007

Biomaterials

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Thermoresponsive polymer (TRP) enables the enzyme-free harvesting of cells through an acute increase in surface hydrophilicity of TRP across its lower critical solution temperature (LCST), rendering feasible the generation of polymer-free cell sheets for regenerative medicine applications. To date, the intricate mechanisms of cell deadhesion/detachment on TRP surface remain obscure. Elucidation of such biophysical responses would be valuable for the cell sheet technology. In this study, integrative biophysical techniques are applied to probe the thermal-induced deadhesion kinetics of smooth muscle cell (SMC) on thermoresponsive hydroxybutyl chitosan (HBC29) against different periods of pre-culture time at 37 °C. Atomic force microscopy demonstrates that both the surface topography and mechanical property of HBC29 film in water are acutely modulated across its LCST. Firstly, cells show negligible changes in adhesion contact area during low-temperature incubation on unmodified tissue culture polystyrene (TCPS). Secondly, the recession of adhesion contact and retraction of cell body for cells with different pre-culture times are triggered by HBC29 coating on TCPS. Interestingly, the initial rate of reduction in the normalized adhesion contact area of SMC is negatively correlated with the pre-culture time. Thirdly, the degree of cell deformation and average adhesion energy are reducing functions of time only for SMCs with the lowest preculture time. In contrast, adhesion energy per cell is a reducing function of time irrespective of the change of pre-culture time. Lastly, the temporal dynamics of cytoskeleton organization and β-actin/ smoothelin-B mRNA expression for SMCs is strongly dependent on the pre-culture time. Overall, this study demonstrates that the thermal-induced deadhesion of SMC on TRP is characterized by the evolution of its contractile phenotypes.

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Changes inβ-actin mRNA Expression in Remodeling Canine Myocardium

Cited by 32 publications

References 0 publications

cDNA Cloning of S100 Calcium-binding Proteins from Bovine Periodontal Ligament and Their Expression in Oral Tissues

cDNA Cloning of S100 Calcium-binding Proteins from Bovine Periodontal Ligament and Their Expression in Oral Tissues

MDR1-targeted siRNA delivery with cationic dendritic conjugated polymers

Dynamics of smooth muscle cell deadhesion from thermosensitive hydroxybutyl chitosan

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