Changes induced by fasting and dietetic obesity in thermogenic parameters of rat brown adipose tissue mitochondrial subpopulations

Matamala, Juan Carlos; Gianotti, Magdalena; Pericàs, Jordi; Quevedo, S.; Roca, Pilar; Palou, Andreu; García-Palmer, Francisco J.

doi:10.1042/bj3190529

Cited by 36 publications

(27 citation statements)

References 41 publications

(62 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…All these results give possible explanation to decreased activity of BAT function and characteristics upon CI treatment, but may not be implicated for changed BAT phenotype. Our results collates very well with the study conducted by Matamala and coworkers, where authors have demonstrated that lack of BAT response in obese rat is due to the change in mitochondria subpopulation and it was different from lean rat (Matamala et al 1996). The above literature provides coherent evidence to suggest that CI treatment may also change the mitochondrial subpopulation leading to decrease the metabolic activity of brown adipocytes.…”

Section: Figuresupporting

confidence: 34%

Chronic hyperinsulinemia reduces insulin sensitivity and metabolic functions of brown adipocyte

Rajan

Shankar

Beg

et al. 2016

Journal of Endocrinology

View full text Add to dashboard Cite

The growing pandemics of diabetes have become a real threat to world economy. Hyperinsulinemia and insulin resistance are closely associated with the pathophysiology of type 2 diabetes. In pretext of brown adipocytes being considered as the therapeutic strategy for the treatment of obesity and insulin resistance, we have tried to understand the effect of hyperinsulinemia on brown adipocyte function. We here with for the first time report that hyperinsulinemia-induced insulin resistance in brown adipocyte is also accompanied with reduced insulin sensitivity and brown adipocyte characteristics. CI treatment decreased expression of brown adipocyte-specific markers (such as PRDM16, PGC1α, and UCP1) and mitochondrial content as well as activity. CI-treated brown adipocytes showed drastic decrease in oxygen consumption rate (OCR) and spare respiratory capacity. Morphological study indicates increased accumulation of lipid droplets in CI-treated brown adipocytes. We have further validated these findings in vivo in C57BL/6 mice implanted with mini-osmotic insulin pump for 8 weeks. CI treatment in mice leads to increased body weight gain, fat mass and impaired glucose intolerance with reduced energy expenditure and insulin sensitivity. CI-treated mice showed decreased BAT characteristics and function. We also observed increased inflammation and ER stress markers in BAT of CI-treated animals. The above results conclude that hyperinsulinemia has deleterious effect on brown adipocyte function, making it susceptible to insulin resistance. Thus, the above findings have greater implication in designing approaches for the treatment of insulin resistance and diabetes via recruitment of brown adipocytes.

show abstract

Section: Figuresupporting

confidence: 34%

Chronic hyperinsulinemia reduces insulin sensitivity and metabolic functions of brown adipocyte

Rajan

Shankar

Beg

et al. 2016

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…A connection between mitochondrial biogenesis and the mitochondrial fractions has been suggested, which would imply that the lightest mitochondria would act as transitional forms of the heaviest, i.e., more differentiated ones [39][40][41][42][43][44]. This fact renders the mitochondrial subpopulations a suitable tool for the study of mitochondriogenesis.…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial Transcription Factor A (TFAM) is Increased in Rat Embryo During Placentation and Associated with Mitochondrial Differentiation

Alcolea¹,

Colom²,

Lladó³

et al. 2006

Cell Physiol Biochem

Self Cite

View full text Add to dashboard Cite

In the current study, the mitochondrial proliferationdifferentiation process was investigated in rat embryo during the placentation process, straight after organogenesis, when there is an important oxidative metabolism activation. For this purpose, on gestational days 11, 12 and 13 we studied the mitochondrial DNA (mtDNA) content and the relative gene expression of proteins involved in mtDNA replication (mitochondrial single strand DNA binding protein (mtSSB)), mtDNA transcription (mitochondrial transcription factor A (TFAM)), as well as in mitochondrial function (cytochrome c oxidase subunit I (COXI)). The results indicated that during placentation important changes in mitochondrial proliferation-differentiation process take place in rat embryo. There is a great decrease in cellular mtDNA content and a rise in the ratio between TFAM and mtDNA accompanied by an increase in COXI gene expression. Thus, we can conclude that on gestational day 13 mitochondrial differentiation predominates over mitochondrial proliferation in embryo cells. Besides, our work reveals that in a physiological condition such as embryonic development the TFAM levels change in order to regulate the transcriptional activity of mtDNA.

show abstract

“…8,20 Obesity affects the expression and regulation of molecules involved in energy homeostasis and body weight maintenance. [21][22][23] Adiponutrin and ATGL mRNA expression has been described to be altered in adipose tissue of animal models of genetic obesity, 2,7,10 and it has been demonstrated that two human adiponutrin polymorphisms are associated with obesity. 9,24 In addition, our group has reported that adiponutrin nutritional regulation is impaired in obese Zucker rats 10 and that fasting upregulation of ATGL in white adipose tissue is impaired in old rats, which could be related to the weight gain that occurs with aging.…”

Section: Introductionmentioning

confidence: 99%

Diet-induced obesity affects expression of adiponutrin/PNPLA3 and adipose triglyceride lipase, two members of the same family

et al. 2011

View full text Add to dashboard Cite

Background: Adiponutrin/PNPLA3 and adipose triglyceride lipase (ATGL) are proteins highly expressed in adipose tissue which have apparently different roles (lipogenic/lipolytic). Gene expression of both proteins and their nutritional regulation have been described to be altered in genetically obese animals. Methods: We studied adiponutrin and ATGL expression in 6-month-old rats made obese by cafeteria diet feeding, submitted to different feeding conditions (feeding/fasting/re-feeding), compared with normoweight animals. Adiponutrin and ATGL mRNA levels were determined in white adipose tissue depots (subcutaneous and visceral) and in interscapular brown adipose tissue, and ATGL protein levels in selected depots. In addition, basal adiponutrin and ATGL expression levels were compared between 6-and 3-month-old animals. Results: Obesity decreased adiponutrin and ATGL expression in different adipose depots. For adiponutrin, a tendency to lower mRNA levels was observed in the white adipose depots studied in obese animals, although the decrease was only significant in the subcutaneous depot. For ATGL, a generalized and significant lower expression was found in white and brown adipose tissue of cafeteria-obese rats. When considering nutritional regulation, according to a lipogenic role, adiponutrin mRNA expression decreased with fasting and was recovered by re-feeding in normoweight animals; this regulation was lost in obese rats. Expression of the lipolytic ATGL (mRNA and protein levels) was increased by fasting in normoweight animals in the mesenteric adipose depot, while no change was evident in obese rats. Moreover, adiponutrin and ATGL nutritional regulation was affected by age, and we report a downregulation of adiponutrin mRNA basal levels with age in internal adipose depots. Conclusions: Cafeteria diet-induced obesity and age alter adiponutrin and ATGL expression and their regulation by feeding conditions. These results reinforce the importance of a proper expression and regulation of both proteins for body weight maintenance and their role in energy metabolism.

show abstract

Changes induced by fasting and dietetic obesity in thermogenic parameters of rat brown adipose tissue mitochondrial subpopulations

Cited by 36 publications

References 41 publications

Chronic hyperinsulinemia reduces insulin sensitivity and metabolic functions of brown adipocyte

Chronic hyperinsulinemia reduces insulin sensitivity and metabolic functions of brown adipocyte

Mitochondrial Transcription Factor A (TFAM) is Increased in Rat Embryo During Placentation and Associated with Mitochondrial Differentiation

Diet-induced obesity affects expression of adiponutrin/PNPLA3 and adipose triglyceride lipase, two members of the same family

Contact Info

Product

Resources

About