Changes in whole cell-derived fatty acids induced by naphthalene in bacteria from genus Pseudomonas

Abstract

Fatty acid composition during naphthalene utilization was investigated in three strains of bacteria Pseudomonas vesicularis, Pseudomonas stutzeri and Pseudomonas sp. JS150 that expressed different naphthalene degradation abilities. All strains significantly changed their cellular fatty acid profiles as a response to naphthalene exposure. Since naphthalene was present in the medium P. stutzeri increased ratio of saturated/unsaturated fatty acids from 1.1 to 2.1 and Pseudomonas sp. JS150 from 7.5 to 12.0, respectively. In contrast, this ratio decreased from 2.1 to 1.1 in P. vesicularis under the same growth conditions. The changes comprised also alterations in the percentage of selected groups of fatty acids: iso and anteiso, hydroxy and cyclopropane fatty acids. Our results showed that naphthalene induced in tested strains different changes in fatty acids composition. It may suggest that in the presence of naphthalene microorganisms used different adaptive mechanisms to maintain the cells in appropriate physiological state.

Closely related intertidal and deep-sea Halomonhystera species have distinct fatty acid compositions

“…We can, however, consider sulphides to be a toxic compound because H. disjuncta is unable to survive concentrations higher than 1.5 mM (personal observations). In the presence of a toxic compound in sublethal concentrations, cells start to decrease membrane fluidity by increasing the degree of FA saturation [17,48,60]. The experimental data, discussed within this paper, allows investigating the individual effects of environmental factors on FA composition.…”

Physiology and transcriptome of the polycyclic aromatic hydrocarbon-degrading Sphingomonas sp. LH128 after long-term starvation

“…As a physiological adaptation, LH128 cells appear to increase the degree of saturation of membrane fatty acids. An increase in the degree of phospholipid fatty acid saturation reduces cell membrane fluidity and permeability and has been previously reported in a number of bacteria as a response to the presence of solutes or toxic hydrocarbons and to a shift in temperature (Heipieper & de Bont, 1994;Heipieper et al, 2003;Johnson et al, 2011;Kieft et al, 1997;Mrozik et al, 2004;Suutari & Laakso, 1994), but has not yet been related to nutrient starvation. In Spingomonas sp.…”

Roseovarius pacificus sp. nov., isolated from deep-sea sediment

“…Cellular fatty acid analysis was carried out as described by Mrozik et al (2004). Analysis of fatty acid methyl esters was performed on a GC-MS (QP2010; Shimadzu) equipped with an RTX-5MS column.…”