Changes in ultrastructural characteristics of endolymphatic sac ribosome-rich cells of the rat during development

Peters, Theo; Tonnaer, E.L.G.M.; Kuijpers, W.; Curfs, J.H.A.J.

doi:10.1016/s0378-5955(02)00748-7

Cited by 8 publications

(9 citation statements)

References 34 publications

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“…In the apical cytoplasm the RRCs were filled with copious amounts of free and bound ribosomes indicating an extensive protein synthesis. Previous data obtained in the rat has demonstrated that the RRCs synthesize and secrete fucosylated glycoproteins and the RRCs are actively involved in endolymph homeostasis through secretion and absorption of complex proteins [Manni and Kuijpers, 1987;Peters et al, 2003]. However, the RRCs have also been proposed to produce a natriuretic peptide and consequently, the ES to have an endocrine function.…”

Section: Discussionmentioning

confidence: 99%

Experimental Hyperactivity of the Endolymphatic Sac

Friis

Thomsen²,

Poulsen³

et al. 2013

Audiol Neurotol

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Injury to the endolymphatic sac may play an important role in the pathogenesis of Ménière’s disease, an inner ear disorder characterized by hearing loss, tinnitus and attacks of vertigo. Isoimmunization of 16 inbred Lewis rats with a crude endolymphatic sac extract and complete Freund’s adjuvant induced hyperactivity of the endolymphatic sac. One group of rats was immunized by a single dose whereas a second group was immunized twice. Control animals were injected with Freund’s adjuvant in saline only. Serum was collected from all rats by the end of the study and harvested autoantibodies were tested by immunohistochemistry. The endolymphatic sacs were investigated by transmission electron microscopy. Endolymphatic sac stimulation was observed in all immunized rats. Based on detailed ultrastructural observations, the degree of reactivity seemed proportional to the number of injections and the extent of immunization. Moreover, the ribosome-rich cells seemed hyperactive with an extravagant content of intracellular components: numerous rough endoplasmic reticulum and free ribosomes, morphological signs of extensive endo- and exocytosis, vesicles of material with a density similar to the homogeneous substance of which many were observed to fuse with primary lysozymes. Basolateral foldings were numerous and in the subepithelial capillaries formation of multiple and apposing fenestrations were observed. No endolymphatic sac stimulation was observed in the control animals. Specific ribosome-rich cell alterations identical to those present in the endolymphatic sac of Ménière’s disease were observed 21 days after the first immunization. The observations suggest that either an autoantigen or a trophic factor, capable of inducing a hyperactivity of the ribosome-rich cells and an imbalance of the homogeneous substance metabolism, exists in the endolymphatic sac of the rat.

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Section: Discussionmentioning

confidence: 99%

Experimental Hyperactivity of the Endolymphatic Sac

Friis

Thomsen²,

Poulsen³

et al. 2013

Audiol Neurotol

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“…For example, amiloride treatment in Bartter syndrome type I, with a defect in the thick ascending limb of the loop of Henle, can considerably decrease the K + loss in the distal tubule and collecting duct. Elucidation of compensatory transport mechanisms in the inner ear (in particular in the endolymphatic sac [49,50,51]) may offer some new perspectives. The inner ear is also an attractive target for gene therapy, because the transgenes may be delivered locally [52,53].…”

Section: Future Perspectivesmentioning

confidence: 99%

Genetic disorders of transporters/channels in the inner ear and their relation to the kidney

Peters¹,

Monnens²,

Cremers³

et al. 2004

Pediatr Nephrol

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Inner ear physiology is reviewed with emphasis on features common to renal physiology. Genetic disorders in transporters/channels for chloride (ClC-K), bicarbonate (Cl(-)/HCO(3)(-) exchanger), protons (H(+)-ATPase), sodium (ENaC, NKKC1, NBC3, NHE3), potassium (KCNQ1/KCNE1, Kcc4), and water (AQP4) in the inner ear and their relation to the kidney are discussed. Based on data from human disorders (with or without mouse counterparts) and mouse models (without human counterparts) this article focuses on the involvement of these transporters/channels in hearing loss.

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“…In the ES of the guinea pig the ultrastructural morphological appearance of both MRC and RRC suggests an active involvement in secretion and degradation of HS, respectively. Peters et al (2003) studied ES cells in detail in the rat. The different subtypes of ES cells were suggested to be dynamically active in endolymph homeostasis.…”

Section: Discussionmentioning

confidence: 99%

“…This is supported by Stankovic et al (1997), who located proton secreting cells in the ES of the guinea pig. Peters et al (2003) also suggested that RRCÕs are actively involved in secretion and absorption of complex proteins. RRCÕs appeared to synthesize and secrete glycoproteins into the ES lumen.…”

Section: Discussionmentioning

confidence: 99%