Changes in Ucp1, D2 (Dio2) and Glut4 (Slc2a4) mRNA Expression in Response to Short-Term Cold Exposure in the House Musk Shrew (Suncus murinus)

Suzuki, Daisuke; Murata, Yoshiharu; Oda, Sen-ichi

doi:10.1538/expanim.56.279

Cited by 6 publications

(4 citation statements)

References 48 publications

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“…BAT activation occurs at room temperatures between 4 - 16°C [9,13,21-23]. In our study, temperature exhibited trivial differences between IH (22.9 ± 2.5°C) and SIH cages (23.2 ± 2.1°C).…”

Section: Discussionmentioning

confidence: 51%

Brown adipose tissue: is it affected by intermittent hypoxia?

et al. 2010

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BackgroundIntermittent hypoxia (IH), a model of sleep apnea, produces weight loss in animals. We hypothesized that changes in brown adipose tissue (BAT) function are involved in such phenomenon. We investigated the effect of IH, during 35 days, on body weight, brown adipose tissue wet weight (BATww) and total protein concentration (TPC) of BAT.MethodsWe exposed Balb/c mice to 35 days of IH (n = 12) or sham intermittent hypoxia (SIH; n = 12), alternating 30 seconds of progressive hypoxia to a nadir of 6%, followed by 30 seconds of normoxia. During 8 hours, the rodents underwent a total of 480 cycles of hypoxia/reoxygenation, equivalent to an apnea index of 60/hour. BAT was dissected and weighed while wet. Protein was measured using the Lowry protein assay.ResultsBody weight was significantly reduced in animals exposed to IH, at day 35, from 24.4 ± 3.3 to 20.2 ± 2.2 g (p = 0.0004), while in the SIH group it increased from 23.3 ± 3.81 to 24.1 ± 2.96 g (p = 0.23). BATww was also lower in IH than in SIH group (p = 0.00003). TPC of BAT, however, was similar in IH (204.4 ± 44.3 μg/100 μL) and SIH groups (213.2 ± 78.7 μg/100 μL; p = 0.74) and correlated neither with body weight nor with BATww. TPC appeared to be unaffected by exposure to IH also in multivariate analysis, adjusting for body weight and BATww. The correlation between body weight and BATww is significant (rho= 0.63) for the whole sample. When IH and SIH groups are tested separately, the correlations are no longer significant (rho= 0.48 and 0.05, respectively).ConclusionIH during 35 days in a mice model of sleep apnea causes weight loss, BATww reduction, and no change in TPC of BATww. The mechanisms of weight loss under IH demands further investigation.

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Section: Discussionmentioning

confidence: 51%

Brown adipose tissue: is it affected by intermittent hypoxia?

et al. 2010

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“…In our present study, we further confirmed that five hours of HHP (0.66 ATA or 18.3% O2) daily for 5 consecutive days per week for 2 weeks before the start of heat exposure caused both weight loss and reduced BAT wet weight in rats. BAT activation occurs at temperatures between 4 and16 °C [51]. In our present study, all HHP experiments were conducted at 26 °C.…”

Section: Discussionmentioning

confidence: 99%

Hypobaric hypoxia preconditioning protects against hypothalamic neuron apoptosis in heat-exposed rats by reversing hypothalamic overexpression of matrix metalloproteinase-9 and ischemia

et al. 2020

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Background: Hypoxia-inducible factor-1α (HIF-1α), heat shock protein-72 (HSP-72), hemeoxygenase-1 (HO-1), and matrix metalloproteinase-9 (MMP-9) have been identified as potential therapeutic targets in the brain for cerebral ischemia. To elucidate their underlying mechanisms, we first aimed to ascertain whether these proteins participate in the pathogenesis of heat-induced ischemic damage to the hypothalamus of rats. Second, we investigated whether hypobaric hypoxia preconditioning (HHP) attenuates heat-induced hypothalamic ischemic/hypoxic injury by modulating these proteins in situ. Methods: Anesthetized rats treated with or without HHP were subjected to heat stress. Hypothalamic ischemic/hypoxic damage was evaluated by measuring hypothalamic levels of cerebral blood flow (CBF), partial oxygen pressure (PO2), and hypothalamic temperature via an implanted probe. Hypothalamic apoptotic neurons were counted by measuring the number of NeuN/caspase-3/DAPI triple-stained cells. Hypothalamic protein expression of HIF-1α, HSP-72, HO-1, and MMP-9 was determined biochemically. Results: Before the start of the thermal experiments, rats were subjected to 5 hours of HHP (0.66 ATA or 18.3% O2) daily for 5 consecutive days per week for 2 weeks, which led to significant loss of body weight, reduced brown adipose tissue (BAT) wet weight and decreased body temperature. The animals were then subjected to thermal studies. Twenty minutes after heat stress, heat-exposed rats not treated with HHP displayed significantly higher core and hypothalamic temperatures, hypothalamic MMP-9 levels, and numbers of hypothalamic apoptotic neurons but significantly lower mean blood pressure, hypothalamic blood flow, and PO2 values than control rats not exposed to heat. In heat-exposed rats, HHP significantly increased the hypothalamic levels of HIF-1α, HSP-72, and HO-1 but significantly alleviated body and hypothalamic hyperthermia, hypotension, hypothalamic ischemia, hypoxia, neuronal apoptosis and degeneration. Conclusions: HHP may protect against hypothalamic ischemic/hypoxic injury and overexpression of MMP-9 by upregulating the hypothalamic expression of HIF-1α, HSP-72, and HO-1 in rats subjected to heatstroke.

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“…Here, we set out to develop a novel cancer therapeutic approach, termed adipose manipulation transplantation (AMT) that utilizes two unique abilities of white adipose tissue (WAT): 1) They can be readily extracted via liposuction and implanted via reconstructive surgery; 2) they can change into a BAT-like tissue, called browning or beiging (16,17), by upregulating essential transcriptional regulators or enzymes, such as the uncoupling protein 1 (UCP1), PPARG coactivator 1 alpha (PPARGC1A) or PR/SET domain 16 (PRDM16), involved in BAT development and function (18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33). Similar to BAT, beige adipocytes have the capacity to convert energy to heat and contribute to whole-body energy expenditure (34).…”

Section: Introductionmentioning

confidence: 99%

Implantation of engineered adipocytes that outcompete tumors for resources suppresses cancer progression

Nguyen

Sheng

Murray

et al. 2023

Preprint

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Tumors acquire an increased ability to obtain and metabolize nutrients. Here, we engineered and implanted adipocytes to outcompete tumors for nutrients and show that they can substantially reduce cancer progression. Growing cells or xenografts from several cancers (breast, colon, pancreas, prostate) alongside engineered human adipocytes or adipose organoids significantly suppresses cancer progression and reduces hypoxia and angiogenesis. Transplanting modulated adipocyte organoids in pancreatic or breast cancer mouse models nearby or distal from the tumor significantly suppresses its growth. To further showcase therapeutic potential, we demonstrate that co-culturing tumor organoids derived from human breast cancers with engineered patient-derived adipocytes significantly reduces cancer growth. Combined, our results introduce a novel cancer therapeutic approach, termed adipose modulation transplantation (AMT), that can be utilized for a broad range of cancers.

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Changes in Ucp1, D2 (Dio2) and Glut4 (Slc2a4) mRNA Expression in Response to Short-Term Cold Exposure in the House Musk Shrew (Suncus murinus)

Abstract: The house musk shrew (Suncus murinus), or so-called suncus, is a coldintolerant mammal, but it is unclear why it is susceptible to low temperatures. Coldintolerance may be the result of lower thermogenic activity in brown adipose tissue (BAT

Cited by 6 publications

References 48 publications

Brown adipose tissue: is it affected by intermittent hypoxia?

Brown adipose tissue: is it affected by intermittent hypoxia?

Hypobaric hypoxia preconditioning protects against hypothalamic neuron apoptosis in heat-exposed rats by reversing hypothalamic overexpression of matrix metalloproteinase-9 and ischemia

Implantation of engineered adipocytes that outcompete tumors for resources suppresses cancer progression

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